The effects of drought stress on morpho-physiological characters and expression of OsCat A in rice seedling
Rahele
Panah Abadi
دانش آموخته کارشناسی ارشد بیوتکنولوژی کشاورزی، گروه بیوتکنولوژی، دانشکده فناوریهای نوین، دانشگاه شهید بهشتی تهران
author
Asadollah
Ahmadi Khah
استادیار گروه بیوتکنولوژی، دانشکده فناوریهای نوین، دانشگاه شهید بهشتی تهران
author
Hossein
Askari
استادیار گروه بیوتکنولوژی، دانشکده فناوریهای نوین، دانشگاه شهید بهشتی تهران
author
text
article
2016
per
Drought stress is one of the most important limiting factors of crop plants in Iran and worldwide. To assess the response of mutant line MT149 to drought stress in comparison to parental cultivar Neda, a factorial experiment was conducted in a completely randomized design. Drought stress (-6 bar) was induced using poly ethylene glycol 6000 and leaf growth dynamics and chlorophyll a and b contents were evaluated in different time series (0, 12, 24 and 36 hours after stress treatment). Results showed that effects of genotype, time and stress were significant on all investigated characters. Also, mean`s comparisons showed that mutant line MT149 was superior over than itself parental counterpart. Furthermore, assessment of catalase gene isoform A (OsCat A) in different times after stress showed that parental cultivar Neda experienced a significant down-regulation of catalase A gene, while MT149 experienced a significant up-regulation of the gene in the same time. Based on the results of this research it can be concluded that one of evidences on a higher drought tolerance of MT149 is probably up-regulation of OsCat A to scavenge the ROS toxicities.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
1
16
https://jab.uk.ac.ir/article_1518_1d79972ce400e463d12c8e77d4daefdb.pdf
dx.doi.org/10.22103/jab.2016.1518
Prion Protein Gene (Prp) Polymorphism in Iranian Ghezel, Harki and Makoei Sheep Breeds Using PCR-SSCP Technique and Sequencing
Vahid
Danesh
دانشجوی دکتری، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Seyyed Hassan
Hafezian
دانشیار، گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Qodratollah
Rahimi Miyanaji
استاد، گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Ayyoub
Farhadi
استادیار، گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
text
article
2016
per
Scrapie or transmissible spongiform encephalopathy (TSE), is an infectious fatal disease of sheep and goats which affects the central nervous system. In the present study, 300 blood samples were collected from three breeds of Iranian indigenous Ghezel, Harki and Makoei sheep breeds. A DNA fragment with the length of 173 bp from exon 3 of PrP gene was amplified with specific primer pairs. The banding pattern of each sample in the study population was identified by means of PCR-SSCP. Then, after sequencing, comparison and analysis of the observed polymorphism between different breeds were performed at codons: 136, 154 and 171. In the present study, tow haplotypes of ARR and ARQ were identified and the wild type scrapie-susceptible ARQ was the most frequent haplotype. In this study more than 98% of animals were with the genotype ARQ/ARQ which was classified as low resistance to this disease. Three novel alleles (L178, E147 and L173) were observed for the first time in this study. The comparison of genotype frequency between Makoei-Ghezel and Makoei-Harki sheep breeds were statistically significant (P<0.05).The data from the current study may help to establish a breeding program for controlling of scrapie in Iranian sheep population.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
17
30
https://jab.uk.ac.ir/article_1519_5843bf23f7b81bfb4f9f68f27e25ae97.pdf
dx.doi.org/10.22103/jab.2016.1519
Callus Induction and Direct Shoot Regeneration in Lepidium draba L. Explants
Zahra
Zinhari
دانش آموخته کارشناسی ارشد رشته بیوتکنولوژی کشاورزی دانشگاه شهید باهنر کرمان
author
Shahram
Pour Seyyedi
دانشیار گروه بیوتکنولوژی کشاورزی دانشگاه شهید باهنر کرمان
author
Jafar
Zolala
استادیار گروه بیوتکنولوژی کشاورزی دانشگاه شهید باهنر کرمان
author
text
article
2016
per
Lepidium draba through the production of anti-cancer and anti-microbial compound, sulforaphane, is considered a valuable medicinal plants.Therefore, optimization of in vitro tissue culture to facilitate genetic transformation and improvement of traits in this plant is important. This study was conducted, by cotyledon, stem and root explants in MS medium in the form of two separate tests. Data were analyzed in a factorial experiment based on completely randomized design. In the first experiment with different concentrations of growth regulators NAA and BAP, the highest response rate, direct shoot regeneration rate and direct shoot generation were related to root explants. The highest root production rate and root generation were showed in cotyledons explants. 1 mgl-1 BAP without NAA induced the highest direct shoot generation in root explants. Cotyledons explants in 2 mgl-1 NAA and 0.5 mgl-1 BAP produced the highest Root production rate and the highest root generation. Callus induction rate and callus initiation of stem explants were more than others, but the callus regeneration rate in three studied explants showed no significant difference. The highest shoot generation from regenerated callus was observed in the cotyledons explants. The second experiment with a combination of different concentrations of 2,4-D and BAP, under the influence of two treatments darkness and 16h photoperiod was performed. The highest investigated traits were in the 16h photoperiod. Cotyledons and stem explants were showed the highest callus induction rate and leaf explants showed the greatest weight of callus; While the highest callus regeneration rate and callus growth rate were obtained from the root explants. From leaf explants, the highest callus weight was obtained from 0.5 mgl-1 2,4-D with 3 mgl-1 BAP and the most growth rate of callus was obtained from 0.5 and 1 mgl-1 2,4-D and 3 mgl-1 BAP. Also the combination of 2 mgl-1 2,4-D with 2 mgl-1 BAP and 3 mgl-1 BAP, produced the highest callus regeneration in root explants.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
31
52
https://jab.uk.ac.ir/article_1520_c295d53c0579aac4321623356303cf08.pdf
dx.doi.org/10.22103/jab.2016.1520
Study of population structure and stratification two ecotypes buffalo with dense single nucleotide polymorphism markers using Admixture, MDS, PCA and GC methods
Zahra
Azizi
دانشجوی دکتری،گروه علوم دامی،دانشکده کشاورزی، دانشگاه تبریز
author
Abbas
Rafat
دانشیار، گروه علوم دامی،دانشکده کشاورزی، دانشگاه تبریز
author
Jalil
Shoja
استاد، گروه علوم دامی،دانشکده کشاورزی، دانشگاه تبریز
author
Hossein
Moradi Shahr Babak
استادیار، گروه علوم دامی،پردیس کشاورزی و منابع طبیعی، دانشگاه تهران
author
Mohammad
Moradi Shahr Babak
استاد، گروه علوم دامی،پردیس کشاورزی و منابع طبیعی، دانشگاه تهران
author
text
article
2016
per
In applications of population genetics, classification of individuals in a sample into populations is important. With the development of high throughput genotyping technologies many markers such as SNPs are available which useful in the study of genetic diversity and structure population. The purpose of this research was to study of population structure and stratification buffaloes from different areas of the two ecotypes (Azari and North) using data SNPChip 90K. A total of 258 buffalo from Ardabil, West Azarbaijan, East Azarbaijan and Guilan provinces were sampled and genotyped. The result showed weak population stratification with λ =1.056 for GC method. Also the plots obtained from PCA and MDS showed separation of different provinces based on genetic distance and these animals have closed genetic relationship. Admixture method represents same results and admixture between individual from different provinces of two ecotypes and k=3 have low error cross validation. These methods are generally able to separate the animals. The results showed the close genetic relationship between two ecotypes from 4 different provinces.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
53
68
https://jab.uk.ac.ir/article_1521_21bda2e4d549f7b21494066a09cc40b3.pdf
dx.doi.org/10.22103/jab.2016.1521
Assessment of artificial operon in Bacillus subtilis to control transcription
Parastoo
Majidian
plant breeding department, Mazandaran agricultural and natural resources research and education center
author
Hamid
Najafi Zarrini
استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران.
author
Ken-ichi
Yoshida
Full professor, Agrobioscience Department, Kobe University, Japan
author
Gholamali
Ranjbar
Plant Breeding and Biotechnology Department, Agricultural Biotechnology
Research Institute of Iran, Sari, Iran
author
Ghorbanali
Nematzadeh
lant Breeding and Biotechnology Department, Agricultural Biotechnology Research
Institute of Iran, Sari, Iran
author
text
article
2016
per
In this study, we proposed the artificial operon which is capable of reversible transcriptional regulation control in Bacillus subtilis. Firstly, the creup and credown were mutated to release from CCR phenomenon. Then, the structural genes of gnt operon were placed under the control of constitutively strong promoter to degrade gluconate constitutively. In addition, the reporter gene (lacZ) was introduced under control of gnt promoters to evaluate functionality of the artificial operon. The results of pulse induction system showed no enzyme activity of each strain in the absence of inducer. By addition of 1 mM gluconate, the highest enzyme activity was shown at 1h after addition of inducer, and then the inducer degraded to turn off the operon. The maximum activation of enzyme was observed in 2h and 4h after addition of 2.5 and 5 mM gluconate into culture media, respectively. Our data indicated that the longer degradation of inducer and higher enzyme activity was appeared by more concentration of gluconate as well as growth arrest. It is suggested to use the pulse induction system and reversible artificial operon to achieve huge amount of special metabolite at short period of bacterial growth.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
69
82
https://jab.uk.ac.ir/article_1522_2c541df78c03bdd90569cf3466194ea7.pdf
dx.doi.org/10.22103/jab.2016.1522
Investigations of linkage disequilibrium phase in chromosome 6 of Holstein breed
Maryam
Nosrati
2Department of Agriculture, Payame Noor University, PO BOX 19395-3697 Tehran, Iran
author
Mojtaba
Tahmoorespur
Professor, Department of animal science, Faculty of agriculture, Ferdowsi University of Mashhad,
Mashhad, Iran
author
Luca
Fontanesi
Department of Agri-Food Science and Technology, University of Bologna, Bologna, Italy
author
Mohamadreza
Nassiri
Professor, Department of animal science, Faculty of agriculture, Ferdowsi University of Mashhad,
Mashhad, Iran
author
text
article
2016
per
In whole genome association study, genomic selection, Determining of extent and level of linkage disequilibrium (LD) is important in sample size and marker density. In this experiment, the blood and sperm samples were collected form 1089 Holstein bulls and 2030 markers on chromosome 6 were genotyped by using Illumina Bovine SNP50K BeadChip based on UMD3.1 genome assembly. Data were corrected for missing genotyped SNP and individual with unknown genotype by Plink. After correction 1606 markers remained (79%). The average observed heterozygosity was 0.37 and 80% of the markers had minor all frequency more than 0.2. The LD was calculated with r2 and Dˊ by Haploview v4.2. The extent of LD in this study was 40 kb with r2=0.3. The r2 value was decreasing to 0.2 (moderate level) in 80kb and 0.1 (background level) in 300 kb. The effective population size has been decreased to 100 individual in 10 generations ago. The 49 haplotype blocks with range 18-472 kb were detected that covering the 10.6 Mb of whole chromosome. These results showed that between 4000-70000 markers or one marker per 40-75 kb will be need for whole genome association study in this Holstein population.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
83
98
https://jab.uk.ac.ir/article_1523_cbc6f379a22efa92183e3e79d25fdaf4.pdf
dx.doi.org/10.22103/jab.2016.1523
Assessment of diversity and genetic relationships in Pomegranate genotypes using AFLP marker
Reza
Nazifi
دانشجوی سابق بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Ghaffar
Kiyani
استادیار گروه بیوتکنولوژی و اصلاح نباتات، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Ali
Dehestani
پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
Seyyed Hamid Reza
Hashemi
پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان، دانشگاه علوم کشاورزی و منابع طبیعی ساری
author
text
article
2016
per
Pomegranate (Punica granatum L.) is native to Iran with several ecotypes which have been adapted to various climatic conditions and diseases. Although it is one of the important exported fruits, there is not a precise identification criteria to determine the authenticity of genetic inheritance and proprietary rights in the country. In the present study the genetic relationships of 47 pomegranate genotypes, including 9 cultivated genotypes (from the collection of Yazd) and 38 wild genotypes (from Mazandaran, Gilan and Golestan provinces), were investigated with AFLP markers. Using 10 primer combinations, 825 bands were obtained of which 697 showed polymorphism. Among the primers, primer combination of M-CTT and E-ACG showed the highest number of bands (148) and primer combination of E-ACC, M-CTG produced the lowest number of bands (40). The average value for PIC was 0.21, while Average Marker Index (MI) was 17.33 that the highest one belonged to primer combination of M-CAC and E-AAG. Maximum genetic similarity belonged to Bahnamir with Shirin Kan Tehran samples and the minimum genetic similarity observed between Berenjestanak and Khosh Darreh. Principal component analysis showed that 57% of the total variance was explained by first three components. The identified primer combinations in this study could be used as a powerful tool for determination of genetic relationships between Pomegranate genotypes
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
99
112
https://jab.uk.ac.ir/article_1524_f68e27e4b65a50d58a4ea81408fb3f23.pdf
dx.doi.org/10.22103/jab.2016.1524
Evaluation of IL-2 and IL-12 mRNA expression in vaccinated guinea pigs with inactivated type O foot-and-mouth disease vaccine
Ebrahim
Honar Bakhsh
دانشآموخته کارشناسی ارشد ژنتیک و اصلاح نژاد دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، ایران
author
Ali Asghar
Aslami Nezhad
دانشیار ژنتیک و اصلاح نژاد دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، ایران
author
Mohammad Reza
Nasiri
استاد ژنتیک و اصلاح نژاد دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، ایران
author
Saeid
Zibaei
استادیار موسسه تحقیقات سرم و واکسن سازی رازی شعبه مشهد، ایران
author
Reza
Pasandide
دانشجوی دکتری ژنتیک و اصلاح نژاد دام، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ملاثانی، اهواز، ایران.
author
text
article
2016
per
Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed livestock and wildlife. Foot-and-mouth disease is an endemic disease in Iran that sometimes caused considerable losses of livestock, especially in young animals. In this study mRNA expression of interleukin-2 (IL-2) and interleukin-12 (IL-12) cytokines was evaluated in vaccinated guinea pigs with inactivated type O FMD vaccine (without adjuvant). Blood samples were collected from saphenous vein of eight male guinea pigs (at control and vaccinated groups) at 7 and 28 days after the first vaccination. Total mRNAs were extracted using the column RNA isolation kit and subsequently reverse-transcribed into cDNA. Then relative real-time PCR assay was used to analyze of quantification of IL-2 and IL-12 expression. The expression of IL-2 gene had not significant difference in vaccinated animals compared to the control group in the first and second blood samples but IL-12 gene expression significantly increased (P<0.05). Evaluation of cytokines genes expression can be as a valuable adjunct for assessment of effects of new vaccines on immune system in future studies.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
8
v.
2
no.
2016
113
126
https://jab.uk.ac.ir/article_1525_8a9343f43c27029a6128c92c504745d5.pdf
dx.doi.org/10.22103/jab.2016.1525