Novel Variants of Citrus Viroid-III in Fars Province
Aliakbar
Bagherian
عضو هیئت علمی دانشگاه جهرم و دانشجوی دکترای دانشکده کشاورزی دانشگاه شیراز
author
keramatollah
Izadpanah
مرکز تحقیقات ویروس شناسی گیاهی دانشکده کشاورزی دانشگاه شیراز
author
text
article
2011
per
Citrus viroid-III (CVd-III) has narrow host range and induces specific symptoms on Etrog citron consist of petiole wrinkle and browning plus leaf twist, leaf droop and bent leaf induced by point necroses of the mid vein. Citrus Viroid-III causes significant reduction of the trunk cross section in Valencia scion and trifoliate rootstock, and the canopy volume of infected trees. In this study a number of citrus trees with or without symptoms were sampled and subjected to RNA extraction followed by RT-PCR, cloning and sequencing of PCR products. Molecular properties (nucleotide composition, primary and secondary structures, molecular weights, phylogenetic relationships and percent nucleotide similarity) of viroid variants were compared. Three novel variants of CVd-III (1, 2 and 3) from Fars were selected and used for comparison with a number of other CVd-III sequences from GenBank. CVd-III-2 had 99 % sequence homology to CVd-III-3 but these two variants had only 93% homology with CVd-III-1. It was shown that on the basis of homology, CVd-III isolates from Fars province clustered in different groups.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
1
14
https://jab.uk.ac.ir/article_457_1bd79e28d33146e26f1ef34806c3b023.pdf
dx.doi.org/10.22103/jab.2012.457
Biological Control of Rhizocronia solani ( Pistachio Isolate) and Molecular Analysis of Chitinase Gene from the Best Bio-Control Agent
Azam
Baharlooee
دانشجوی کارشناسی ارشد بخش بیوتکنولوژی کشاورزی، دانشگاه شهید باهنر کرمان
author
GHolamreza
sharifi-sirchi
استادیار بخش بیوتکنولوژی کشاورزی، دانشگاه شهید باهنر کرمان
author
text
article
2011
per
Rhizoctonia solani, the causal agent of damping-off, root rot and stem rot, is one of the most important phytopathogenes with a wide host range which causes significant reduction in yield of pistachio (Pistacia vera). In search for finding chitinolytic biocontrol agents, actinomycetes producing chitinase enzyme were isolated from agricultural soils of Sirch, Kerman province . From tested isolates, Streptomyces isolate 410 significantly reduced the incidence of disease. Chitinase gene (603 bp) was cloned, sequenced and characterized from this isolate. The results showed that nucleotides encoding 200 amino acids and molecular weight of 21636.9 Da. This protein had the high homology with the family 19 chitinases glycosyl hydrolases which have the major role in the defense response against phytopathogens
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
15
30
https://jab.uk.ac.ir/article_458_89608780da1b46a3bdd120fbd30cb4de.pdf
dx.doi.org/10.22103/jab.2012.458
Unexpected Transcription of Human t-PA cDNA in transgenic tobacco plants
Mokhtar
jalali
1عضو هیئت علمی دانشکده کشاورزی دانشگاه تربیت مدرس
author
Heydar
Seifi nabiabad
دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشگاه تربیت مدرس
author
Mohammad mehdi
Yaghoobi
عضو هیئت علمی مرکز بین المللی علوم، تکنولوژی پیشرفته و علوم محیطی
author
Saman
Hossein khani
عضو هیئت علمی دانشکده علوم زیستی دانشگاه تربیت مدرس
author
text
article
2011
per
Recombinant tissue-type plasminogen activator (rt-PA) is used as a fibrin-specific thrombolytic agent for the treatment of various thromboembolic diseases. In this study, we tried to show the expression of transferred cDNA of human t-PA gene in transgenic tobacco plants produced previously by this group. RT-PCR with specific primers for t-PA, were used to assay the transgenic plants. RT-PCR products were observed on agarose gel electrophoresis after ethidium bromide staining. The results showed that the t-PA gene is stably expressed in second generation transgenic plant at the level of mRNA, while no expression was seen in non transgenic plants. These results also indicate an unfamiliar 650 bp transcript from t-PA transgene in plants. The results of homology search by CDD at NCBI database demonstrate that in addition to complete cDNA, an incomplete transcript so observed. This truncated transcript is a part of cDNA of t-PA that has lost the kringles domain while tyrosine-like serine protease is remained. This may results from alternative RNA splicing. The serine protease domain can carry out the role of t-PA alone. So many scientists are trying to clone this fragment only as a drug. Interestingly the plant produces this fragment naturally. This result may be because of alternative splicing of RNA.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
31
41
https://jab.uk.ac.ir/article_459_571c77df5c6a0e2dad140513f0d596cd.pdf
dx.doi.org/10.22103/jab.2012.459
The Effect of Different Light Intensities on Callogenesis and Calli Pigments Content of Shoot and Floral Explants of Rosa damascena Mill. and Rosa miniature
Somayeh
Abdirad
فارغ التحصیل مقطع کارشناسی ارشد، بخش زیست شناسی، دانشگاه شهید باهنر کرمان
author
Farkhondeh
Reza nejad
دانشیار بخش زیست شناسی، دانشگاه شهید باهنر کرمان
author
KHosroo
Manoochehri
استاد بخش زیست شناسی، دانشگاه شهید باهنر کرمان
author
text
article
2011
per
This study was conducted to compare the ability of callus production between shoot (leaf, petiole and stem) and flower (petal, pistil and anther) organs, as well as chlorophyll and anthocyanin accumulation in calli obtained from these explants in dark and light in Rosa miniature and Rosa damascena. Miniature rose and damask rose have become increasingly popular and economically important in recent years. In vitro regeneration method has a great commercial value in the rose propagation industry. The sterilized explants were cultured in modified MS medium supplemented by various concentrations of 2, 4-D (1, 2, 3, and 4 mg.l-1) and BAP (0.1, 0.5, 1 and 1.5 mg.l-1). The samples were kept under 1000 and 2000 lux white light intensities and dark. Combinations of 4 mg l-1 2, 4-D and 1.5 mg l-1 BAP in Rosa damascena were the most suitable treatments for callus production whereas various explants of Rosa miniature had different response to different growth regulator combinations. In the dark, callus initiation was quicker compared to the light. In addition, callus production of shoot explants were begun faster than floral ones. Callus initiation of floral explants from Rosa miniature occurred in 2th week after culturing, whereas in Rosa damascena observed in 3th week. The growth rate of calli from all explants (except anther) of Rosa miniature was more than Rosa damascena. The dark grown calli contained very low chlorophyll and anthocyanin concentrations whereas these contents increased remarkably at 1000 and 2000 lux (especially 2000 lux) light intensity. Leaf calli in Rosa damascena and stem calli in Rosa miniature produced maximum values of chlorophyll pigments whereas in both, anther and petiole calli had minimum amounts. The calli obtained of petal and pistil explants accumulated high concentration of anthocyanin pigments whereas the lowest values recorded from anther and petiole calli in Rosa miniature and petiole calli in Rosa damascena.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
43
65
https://jab.uk.ac.ir/article_460_29f5592a1999a35b158515645fe9ab02.pdf
dx.doi.org/10.22103/jab.2012.460
Study of genetic diversity and eco-geographic groups in some apricot cultivars and genotypes using flurescent-AFLP markers
Parastoo
Majidian
دانشجوی کارشناسی ارشد، بخش زراعت و اصلاح نبات، دانشگاه کشاورزی و منابع طبیعی ساری
author
Mehrshad
Zeinolabedini
استادیار بخش ژنومیکس، انستیتو تحقیقاتی بیوتکنولوژی کشاورزی ایران
author
Jalil
Degam poor
استادیار مرکز تحقیقات کشاورزی آذربایجان شرقی
author
Hamid
Najafi
استادیار بخش زراعت و اصلاح نبات، دانشگاه کشاورزی و منابع طبیعی ساری
author
Mohssen
Mardi
دانشیار بخش ژنومیکس، انستیتو تحقیقاتی بیوتکنولوژی کشاورزی ایران
author
Mohsen
Dabab
دانشجوی کارشناسی ارشد، بخش ژنومیکس، انستیتو تحقیقاتی بیوتکنولوژی کشاورزی ایران
author
Maryam
Farsi
دانشجوی کارشناسی ارشد، بخش ژنومیکس، انستیتو تحقیقاتی بیوتکنولوژی کشاورزی ایران
author
text
article
2011
per
Apricot (Prunus armenica L.), a fruit species of the family Rosaceae, genus Prunus is widely distributed in the Mediterranean region and the Middle East, especially in Iran. Suitable climatic conditions for apricot production and its economic importance were the main reasons for its vast area under cultivation and diversity. Genetic diversity of 42 apricot cultivars and genotypes collected from new Iranian and Czech Republic collections was studied using fluorescent-AFLP markers. The objectives of this study were to determine the genetic structure and genotypic diversity among different eco-geographical populations. The results showed that five pairs of fluorescent-AFLP primers showed clear banding patterns and high level of polymorphism amongst the 30 pairs of primers screened. The total bands were 187 and MGAG ETG primer showed 63% polymorphism among primers that was the highest quantity of polymorphism. Based on the application of genotypes from breeding programs, Hybridization and selection, there were not appropriate results from UPGMA method. We used RB method to analyze the relationship among samples. Six groups of cultivars and genotypes were detected based on their origin and geographical distribution. Our findings showed that in comparison with traditional AFLP, fluorescent-AFLP markers are more efficient in evaluating genetic diversity and population structure among studied apricot genotypes and could be useful in other Prunus fruit tree species
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
67
76
https://jab.uk.ac.ir/article_461_8549611008f2d38355434caca44a89fc.pdf
dx.doi.org/10.22103/jab.2012.461
Study and comparative of polymorphism (T487C) in exon 17 of the DGAT1 gene and its relationship with carcass weight and dressing percentage in the Lori-Bakhtiari and Zel sheep breed
Hossin
Mohammadi
دانش آموخته کارشناسی ارشد ژنتیک و اصلاح نژاد دام، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
author
Mohammad
Moradi
استادپردیس کشاورزی و منابع طبیعی، دانشگاه تهران
author
Mostafa
Sadeghi
استادیار پردیس کشاورزی و منابع طبیعی، دانشگاه تهران
author
text
article
2011
per
Diacylglycerol acyltransferase 1 (DGAT1) is one of the candidate genes to improve carcass characteristics in feedlot animals. Gene product coding, a microsomal enzyme that final triglyceride synthesis, hence converting diacyl glycerol to triglyceride accelerated. The purpose of this research, study area (T487C) in exon 17 of the above gene polymorphisms with carcass traits in two Lori-Bakhtiari and Zel breed. For this purpose, during the 35 period animals randomly slaughtered in the abattoir were recorded. DNA was extracted using salting-out method, 152 samples of Lori-Bakhtiari sheep and 157 samples of Zel sheep were obtained, polymerase chain reaction to amplify 309 bp of exon 17 DGAT1 gene using a pair of specific primers was performed. Genotypes obtained from method PCR-RFLP and directly from agarose gel. In Lori-Bakhtiari sheep in two alleles T and C with frequency of 0.746 and 0.254 respectively, were observed, in population Zel Sheeps the two T and C, with frequency 0.81 and 0.19 respectively, observed. Statistical analysis showed polymorphism in exon 17 region of the gene significantly correlated with carcass weight and dressing percentage (P < 0.05). So that the CC genotypes of the significant mean carcass weight and dressing percentage heavier than had TT genotypes (P< 0.05). Of polymorphism can be observed that improvement in breeding programs to improve carcass weight and dressing percentage through selection in favor of superior genotypes be used.
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
77
89
https://jab.uk.ac.ir/article_462_cbb372de0b96dfa0f69252c0c8ff26e6.pdf
dx.doi.org/10.22103/jab.2012.462
Genetic and phylogenetic analysis of cytochrome b region in Jebeer of Iran
Mohammadreza
Nassiri
دانشیار، گروه علوم دامی و پژوهشکده زیست فناوری، دانشگاه فردوسی مشهد
author
Morteza
Mahdavi
دانشجوی مقطع دکتری، گروه علوم دامی، دانشگاه فردوسی مشهد
author
text
article
2011
per
Jebeer is belonging to subfamily of mountain goats (Antilopinae), genus of Gazelle (Gazella) and found mainly in central and southern desert areas of Iran. There are many challenges between researchers about determining Jebeer species. Whereas determining Jebeer species is first step in protecting of them we perform this research. In this regard 5 biological samples from Shirahmad’s wild life refuge of Sabzevar were collected and cytochrome b region of mitochondrial DNA have been amplified with L14724 and H15915 primers. Resulting fragments were sequenced with ABI 3130 automated device with Sanger method. These sequences compared with 190 sequences from Gazella genus that classified according to species with regard to homology and genetic distances, then phylogenetic tree have been drawn. Results show that among all Gazelle sp. Jebeer have least distance equal to 0.003 with G. bennetti and most distance with G. dorca and his sister taxon G. saudiya that is equal to 0.073 and 0.074 respectively. Thereby can conclude that Jebeer is belonging to G. bennetti species not G. dorcas
Agricultural Biotechnology Journal
Shahid Bahonar University of Kerman and Iranian Biotechnology Society
2228-6705
3
v.
1
no.
2011
91
104
https://jab.uk.ac.ir/article_463_5ec89ac75d7a303ace40c5c0b1d0edd5.pdf
dx.doi.org/10.22103/jab.2012.463