Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 6 2 2014 08 23 Study of Genetic Diversity of Iranian Thymus Accessions, Using ISJ Semi-random Markers Study of Genetic Diversity of Iranian Thymus Accessions, Using ISJ Semi-random Markers 147 162 1318 10.22103/jab.2014.1318 FA Farzaneh Mojiri Ahmad Esmaeili Farhad Nazarian Firouz Abadi Hassan Maddahi Arefi Hadi Ahmadi Journal Article 2012 12 04 Thymus is one of the famous medicine herbs of Lamiaceae genus. Due to the high potential of hybridization and introgression among thymus species, they have high genetic variability and study of genetic variability is difficult in this genus. According to the existing problems in the field of breeding of this medicine herb, use of molecular markers could be a valuable tool for evaluation and exploitation of germplasm. In this study, for assessment of genetic diversity among 70 Iranian Thymus, 30 semi-random ISJ (Intron-exon Splicing Junction) primers were used. Total primers produced 694 bands that 683 bands were polymorphic. Average band number per primer and per genotype was 23.13 and 9.91, respectively. Cluster analysis using DARwin5 software and UPGMA method based on Dice's similarity matrix divided accessions into 6 clusters. The highest similarity was estimated between T. kotschyanus and T. transcaucasius and lowest similarity was estimated between T. lancifolius and T. fedtschenkoi. The highest and lowest of polymorphic information content (PIC) revealed by IT15-36 and IT18-2 primers, respectively. The highest and lowest marker index (MI) included ET15-33 and ET18-6 primers, respectively. Results showed that clustering based on cluster analysis partly adapted with geographical origin dispersion. Totally, application of semi-random primers could be useful in assessment of genetic diversity of Thymus accessions. Thymus is one of the famous medicine herbs of Lamiaceae genus. Due to the high potential of hybridization and introgression among thymus species, they have high genetic variability and study of genetic variability is difficult in this genus. According to the existing problems in the field of breeding of this medicine herb, use of molecular markers could be a valuable tool for evaluation and exploitation of germplasm. In this study, for assessment of genetic diversity among 70 Iranian Thymus, 30 semi-random ISJ (Intron-exon Splicing Junction) primers were used. Total primers produced 694 bands that 683 bands were polymorphic. Average band number per primer and per genotype was 23.13 and 9.91, respectively. Cluster analysis using DARwin5 software and UPGMA method based on Dice's similarity matrix divided accessions into 6 clusters. The highest similarity was estimated between T. kotschyanus and T. transcaucasius and lowest similarity was estimated between T. lancifolius and T. fedtschenkoi. The highest and lowest of polymorphic information content (PIC) revealed by IT15-36 and IT18-2 primers, respectively. The highest and lowest marker index (MI) included ET15-33 and ET18-6 primers, respectively. Results showed that clustering based on cluster analysis partly adapted with geographical origin dispersion. Totally, application of semi-random primers could be useful in assessment of genetic diversity of Thymus accessions.

https://jab.uk.ac.ir/article_1318_29912c4c29e1929f04cb9232beb6b938.pdf