Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 6 3 2014 11 22 Detection of some bacterial causes of abortion in Afshari sheep using Real Time PCR detection and sensitivity assessment of Campylobacter primers Detection of some bacterial causes of abortion in Afshari sheep using Real Time PCR detection and sensitivity assessment of Campylobacter primers 107 120 1330 10.22103/jab.2014.1330 FA Masoumeh Saleh Mohammad Taher Haraki Nezhad Vahid Salmani Journal Article 2013 05 07 The present a method for fast and accurate detection of abortion bacterial agents is the most priority of control and treatment for this disease. The aim of this study was the optimization of Real Time PCR for simultaneous detection of four of the most important bacteria causing abortion (Campylobacter, Brucella, Yersinia and Salmonella). 217 vaginal swab samples (132 samples from aborted and 85 samples from non-aborted ewes) were collected from some rural regions of Zanjan province. Pure samples of Brucella, Yersinia and Salmonella were also purchased from Persian Type Culture Collection (PTCC). After DNA extraction, specific primers of Brucella, Salmonella and Yersinia were examined on each purchased bacteria DNA sample. With 68 positive samples from 132 aborted ewes and 29 positive samples from 85 healthy ewes, Campylobacter was distinguished as a main factor of abortion in rural areas of Zanjan. The precision of Campylobacters primer was also assessed using Real Time PCR in different dilutions. The results showed that all bacteria in the same condition concurrently were amplified with Real Time PCR using specific primers and Campylobacters primer is able to identify and amplifying of bacteria in all dilutions even when just one copy is available. The present a method for fast and accurate detection of abortion bacterial agents is the most priority of control and treatment for this disease. The aim of this study was the optimization of Real Time PCR for simultaneous detection of four of the most important bacteria causing abortion (Campylobacter, Brucella, Yersinia and Salmonella). 217 vaginal swab samples (132 samples from aborted and 85 samples from non-aborted ewes) were collected from some rural regions of Zanjan province. Pure samples of Brucella, Yersinia and Salmonella were also purchased from Persian Type Culture Collection (PTCC). After DNA extraction, specific primers of Brucella, Salmonella and Yersinia were examined on each purchased bacteria DNA sample. With 68 positive samples from 132 aborted ewes and 29 positive samples from 85 healthy ewes, Campylobacter was distinguished as a main factor of abortion in rural areas of Zanjan. The precision of Campylobacters primer was also assessed using Real Time PCR in different dilutions. The results showed that all bacteria in the same condition concurrently were amplified with Real Time PCR using specific primers and Campylobacters primer is able to identify and amplifying of bacteria in all dilutions even when just one copy is available.

https://jab.uk.ac.ir/article_1330_4785ad3a0f35c66b00389f79248c3653.pdf