Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 6 4 2015 02 20 Identification of quantitative trait loci associated with weight and percentage of internal organs on chromosome 1 in Japanese quail Identification of quantitative trait loci associated with weight and percentage of internal organs on chromosome 1 in Japanese quail 143 158 1346 10.22103/jab.2015.1346 FA Hassan Moradian Ali Esmaeili Zadeh Mohmmad Reza Mohammad Abadi Saeid Sohrabi Journal Article 2013 04 27 The purpose of this study was to identify genomic regions affecting weight and percentage of internal organs on chromosome 1 in an F₂ population of Japanese quail. A three-generation resource population was developed by using two distinct Japanese quail strains, wild (meat type) and white (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F₁ birds were produced. The F₁ birds were intercrossed to generate 422 F₂ offspring. Phenotypic data including weight of organs were collected on F₂ birds. All of the animals from three generations (472 birds) were genotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with least square regression interval mapping method fitting three various statistical models. Significant QTL were identified for pre-stomach weight, uropygial gland weight, bursa of fabricius weight, gizzard weight, percentage of pre-stomach, percentage of intestine, percentage of uropygial gland and percentage of bursa of fabricius. The proportion of the F₂ phenotypic variation explained by the significant additive, dominance and imprinted QTL effects ranged from 4.06 to 7.29%, 1.96 to 4.65% and 2.7 to 6.25%, respectively. The results of this study show that there are quantitative trait loci associated with weight and percentage of internal organs on chromosome 1 in Japanese quail, but more studies are needed to better understand genetic structure of these traits. The purpose of this study was to identify genomic regions affecting weight and percentage of internal organs on chromosome 1 in an F₂ population of Japanese quail. A three-generation resource population was developed by using two distinct Japanese quail strains, wild (meat type) and white (layer type). Eight pairs of white and wild birds were crossed reciprocally and 34 F₁ birds were produced. The F₁ birds were intercrossed to generate 422 F₂ offspring. Phenotypic data including weight of organs were collected on F₂ birds. All of the animals from three generations (472 birds) were genotyped for eight microsatellite markers on chromosome 1. QTL analysis was performed with least square regression interval mapping method fitting three various statistical models. Significant QTL were identified for pre-stomach weight, uropygial gland weight, bursa of fabricius weight, gizzard weight, percentage of pre-stomach, percentage of intestine, percentage of uropygial gland and percentage of bursa of fabricius. The proportion of the F₂ phenotypic variation explained by the significant additive, dominance and imprinted QTL effects ranged from 4.06 to 7.29%, 1.96 to 4.65% and 2.7 to 6.25%, respectively. The results of this study show that there are quantitative trait loci associated with weight and percentage of internal organs on chromosome 1 in Japanese quail, but more studies are needed to better understand genetic structure of these traits. https://jab.uk.ac.ir/article_1346_817b492480fdf38169d6a863ae736ef2.pdf