Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 8 3 2016 11 21 Identification of retrotransposon-based (IRAP) loci associated with resistance to Sclerotinia stem rot disease (Sclerotinia spp.) in sunflower Identification of retrotransposon-based (IRAP) loci associated with resistance to Sclerotinia stem rot disease (Sclerotinia spp.) in sunflower 97 118 1550 10.22103/jab.2016.1550 FA Roghayeh Najafzadeh Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran and National Elites Foundation, Tehran, Iran and Assistant Professor, Department of Medicinal Plants, Shahid Bakeri Higher Education Center, Urmia University, Miyandoab, Iran Reza Darvishzadeh Professor, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran and Institute of Biotechnology, Urmia University, Urmia, Iran Khadijeh Musa-Khalifani M.Sc. Graduate, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran Masood Abrinbana Asistant Professor, Department of Plant Protection, Urmia University, Urmia, Iran Journal Article 2016 09 03 Sclerotinia disease is important fungal disease of sunflower inIranthat reduces its growth and yield. In this study, reactions of 100 oily sunflower lines to 6 fungal isolates of Sclerotinia were studied and then 128 retrotransposon-based molecular markers (IRAP) were used to identify genes controlling disease resistance. The results showed that there is high diversity in the studied germplasm for resistance to the Sclerotinia stem rot. Population structure analysis subdivided the studied lines into 3 subpopulations (K=3). Association analysis with general and mixed linear models (GLM and MLM) identified 14 and 11 loci, respectively that are significantly associated (P≤0.01) with Sclerotinia disease resistant genes. Markers LTR 1063-65, LTR 1064-65 and LTR 1062 were commonly associated with resistant to some fungal isolates. The common markers due to facilitating simultaneous selection for resistance to several fungal isolates can be well used in sunflower breeding programs like marker-assisted selection (MAS). Sclerotinia disease is important fungal disease of sunflower inIranthat reduces its growth and yield. In this study, reactions of 100 oily sunflower lines to 6 fungal isolates of Sclerotinia were studied and then 128 retrotransposon-based molecular markers (IRAP) were used to identify genes controlling disease resistance. The results showed that there is high diversity in the studied germplasm for resistance to the Sclerotinia stem rot. Population structure analysis subdivided the studied lines into 3 subpopulations (K=3). Association analysis with general and mixed linear models (GLM and MLM) identified 14 and 11 loci, respectively that are significantly associated (P≤0.01) with Sclerotinia disease resistant genes. Markers LTR 1063-65, LTR 1064-65 and LTR 1062 were commonly associated with resistant to some fungal isolates. The common markers due to facilitating simultaneous selection for resistance to several fungal isolates can be well used in sunflower breeding programs like marker-assisted selection (MAS). https://jab.uk.ac.ir/article_1550_764317684bcae217cb7f4cdb483d05c5.pdf