Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 8 4 2017 02 19 Studying polymorphism in exon 17 of the DGAT1 gene and its association with milk fat percentage in the Kurdi sheep breed of Shirvan Studying polymorphism in exon 17 of the DGAT1 gene and its association with milk fat percentage in the Kurdi sheep breed of Shirvan 57 68 1633 10.22103/jab.2017.1633 FA Zeynab Rahmani M.Sc. graduate and Associate Professor, Department of Animal and Poultry Breeding and Genetics, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Saied Hasani M.Sc. graduate and Associate Professor, Department of Animal and Poultry Breeding and Genetics, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Saeid Zerehdaran Professor, Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran. Alireza Khan Ahmadi Assistant Professor, Department of Animal Science, Gonbad University, Gonbad, Iran. Journal Article 2016 10 13 Diacylglycerol acyltransferase 1 (<em>DGAT1</em>) is a microsomal enzyme that accelerates the final stage of the synthesis of triglycerides, means converting diacylglycerol to triacylglycerol. The aim of this study was to investigate polymorphism in exon 17 of the <em>DGAT1</em> gene and their associations with milk fat percentage in Kurdi sheep breed in Hussein Abad breeding station of Shirvan. Hence, milk records of 100 ewes were randomly selected and milk fat percentage was measured by Total Eco milk device. Indeed, ewe DNA was extracted and amplified by specific primers for a 309 bp fragment of exon 17 of this gene. Genotypes were determined by PCR-RFLP digested with <em>AluI</em> endonuclease enzyme. Frequencies of C and T alleles were 0.65 and 0.35, respectively. Statistical analysis was conducted by GLM procedure of SAS 9.1 software and showed significant association of genotypes with milk fat percentage (PDGAT1 to increase sheep milk fat percentage in marker assisted selection programs. Diacylglycerol acyltransferase 1 (<em>DGAT1</em>) is a microsomal enzyme that accelerates the final stage of the synthesis of triglycerides, means converting diacylglycerol to triacylglycerol. The aim of this study was to investigate polymorphism in exon 17 of the <em>DGAT1</em> gene and their associations with milk fat percentage in Kurdi sheep breed in Hussein Abad breeding station of Shirvan. Hence, milk records of 100 ewes were randomly selected and milk fat percentage was measured by Total Eco milk device. Indeed, ewe DNA was extracted and amplified by specific primers for a 309 bp fragment of exon 17 of this gene. Genotypes were determined by PCR-RFLP digested with <em>AluI</em> endonuclease enzyme. Frequencies of C and T alleles were 0.65 and 0.35, respectively. Statistical analysis was conducted by GLM procedure of SAS 9.1 software and showed significant association of genotypes with milk fat percentage (PDGAT1 to increase sheep milk fat percentage in marker assisted selection programs. https://jab.uk.ac.ir/article_1633_673562f8d75fcde8fc84f437ec987c76.pdf