Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 9 1 2017 06 22 Cloning of human insulin-like growth factor gene in plant expression vectors Cloning of human insulin-like growth factor gene in plant expression vectors 15 30 1666 10.22103/jab.2017.1666 FA Sargol Darabi MSc student, Azarbaijan Shahid Madani University, Tabriz, Iran Sonia Ramazani MSc student, Azarbaijan Shahid Madani University, Tabriz, Iran Mohammad Ahmadabadi Assistant professor, Azarbaijan Shahid Madani University, Tabriz, Iran Journal Article 2013 10 21 Transgenic plants are suitable bioreactors for the production of recombinant proteins at high levels and qualities. Cloning of a gene encoding the target protein in proper expression vectors is one of the most important steps for successful integration and expression of target protein in plants. The purpose of this study was to clone insulin-like growth factor -1 (IGF1) cDNA in plant expression vectors. To this end, we amplified amplify IGF1 cDNA using a pair of specific primer followed by its cloning in pMCS5 basic vector. After sequencing, because of several advantages of cereals for production of biopharmaceuticals, such as high cultivation area and biomass, possibility of grain storage and usage as food and feed, we constructed suitable vectors for <em>IGF1</em> integration and expression in cereals. In addition, as transgenic chloroplasts contain higher potential to produce elevated levels of functional recombinant proteins, the <em>IGF1</em> cDNA was recloned in two chloroplast-specific vectors. In one of these vectors, selectable marker gene is flanked by two direct repeats of LoxP sequences, providing the marker gene excision after production of transgenic plants via Cre/Lox system. Transgenic plants are suitable bioreactors for the production of recombinant proteins at high levels and qualities. Cloning of a gene encoding the target protein in proper expression vectors is one of the most important steps for successful integration and expression of target protein in plants. The purpose of this study was to clone insulin-like growth factor -1 (IGF1) cDNA in plant expression vectors. To this end, we amplified amplify IGF1 cDNA using a pair of specific primer followed by its cloning in pMCS5 basic vector. After sequencing, because of several advantages of cereals for production of biopharmaceuticals, such as high cultivation area and biomass, possibility of grain storage and usage as food and feed, we constructed suitable vectors for <em>IGF1</em> integration and expression in cereals. In addition, as transgenic chloroplasts contain higher potential to produce elevated levels of functional recombinant proteins, the <em>IGF1</em> cDNA was recloned in two chloroplast-specific vectors. In one of these vectors, selectable marker gene is flanked by two direct repeats of LoxP sequences, providing the marker gene excision after production of transgenic plants via Cre/Lox system. https://jab.uk.ac.ir/article_1666_277a05572df2af99e58cc9b60c9eb4a1.pdf