Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 9 4 2018 02 20 Promoter Analysis of S-Like RNase gene in Transgenic Rice Promoter Analysis of S-Like RNase gene in Transgenic Rice 65 80 2015 10.22103/jab.2018.2015 FA Rudabeh Ravash Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekod, Iran. 0000-0001-7321-6337 Behrooz Shiran Plant Breeding and Biotechnology Department, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran Esmail Ebrahimi Professor, Plant Breeding and Biotechnology Department, Faculty of Agriculture, Shiraz University, Shiraz, Iran Sadolaah Hooshmand Professor, Plant Breeding and Biotechnology Department, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran Rudy Dolferus Professor, CSIRO Plant Industry, Canberra-Australia Journal Article 2016 12 27 RNases participate in vital cellular functions such as DNA replication, transcription, splicing, and control of translation. S-like ribonucleases (S-like RNases) are homologous to S-ribonucleases (S-RNases), but are not involved in self-incompatibility in plants. In dicotyledonous plants, S-like RNases play an important role in phosphate recycling during senescence and are induced by inorganic phosphate-starvation and in response to defense and mechanical wounding. Potensial effects of RNases in drought response might refer to intraction of several different functions. In this study, the promoter of S-Like RNase gene was inserted upstream of β-glucuronidase gene (GUS), Agrobacterium tumefaciens, transformed by electroporation with these constructs were used for co-culture for plant transformation. Histochemical staining method on root, Leaf and anther tissue of rice were done for measering promoter activity. Based on these results, we report tissue speciefic activity of S-Like RNase promoter in the anther of rice. Results indicate the high activity of S-Like RNase promoter in the anther tissue of rice however Expression level in the leaf was low and in the root was negligible. These results is confirmed by previeos studies using q-PCR that indicated high expression of S-Like RNase gene at the anther in comparison with other tissues. RNases participate in vital cellular functions such as DNA replication, transcription, splicing, and control of translation. S-like ribonucleases (S-like RNases) are homologous to S-ribonucleases (S-RNases), but are not involved in self-incompatibility in plants. In dicotyledonous plants, S-like RNases play an important role in phosphate recycling during senescence and are induced by inorganic phosphate-starvation and in response to defense and mechanical wounding. Potensial effects of RNases in drought response might refer to intraction of several different functions. In this study, the promoter of S-Like RNase gene was inserted upstream of β-glucuronidase gene (GUS), Agrobacterium tumefaciens, transformed by electroporation with these constructs were used for co-culture for plant transformation. Histochemical staining method on root, Leaf and anther tissue of rice were done for measering promoter activity. Based on these results, we report tissue speciefic activity of S-Like RNase promoter in the anther of rice. Results indicate the high activity of S-Like RNase promoter in the anther tissue of rice however Expression level in the leaf was low and in the root was negligible. These results is confirmed by previeos studies using q-PCR that indicated high expression of S-Like RNase gene at the anther in comparison with other tissues.

https://jab.uk.ac.ir/article_2015_65d2ea03425887a717c435081cfc5dbb.pdf