Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 11 2 2019 08 23 Effect of DL- Methionine Replacement with L- Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails Effect of DL- Methionine Replacement with L- Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails 23 36 2386 10.22103/jab.2019.13274.1099 FA Kei Aram Koohgivi MS graduate of Animal genetic and Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Iran Hedaiat Allah Rooshanfekr Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran 0009-0008-6988-7240 Mahmood Nazari Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran Ahmad Tatar Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran Journal Article 2019 02 24 Objective Myostatin also known as growth differentiation factor 8, is a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. This research had been done in order to assess the effect of DL-methionine replacement with L-methionine, and also the effects of varying dietary protein levels on Myostatin gene expression in Japanese quail. Materials and methods This experiment was carried in the form of a 2×2 factorial with 4 treatment. Treatments contained the replacement of DL-methionine with L- methionine, and different dietary protein levels, were 24 and 20 %. After about 35 days of feeding and keeping the quails, a piece of their chest has been removed immediately and were transferred to the laboratory. Myostatin gene expression measured by using RT-qPCR technique. In this method, 𝜷-actin gene was used as a house-keeping gene to normalize the gene expression data in the quantitative real time PCR. Results DL-methionine replacement did not significantly effect on Myostatin gene expression. Whereas, reduction in protein surface from 24% to 20 %, led to significantly increased expression of Myostatin (P<0.01). Conclusions The results indicated that DL methionine could be replaced with L methionine, and the appropriate level of protein was 24% in the Japanese quail diet. Objective Myostatin also known as growth differentiation factor 8, is a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. This research had been done in order to assess the effect of DL-methionine replacement with L-methionine, and also the effects of varying dietary protein levels on Myostatin gene expression in Japanese quail. Materials and methods This experiment was carried in the form of a 2×2 factorial with 4 treatment. Treatments contained the replacement of DL-methionine with L- methionine, and different dietary protein levels, were 24 and 20 %. After about 35 days of feeding and keeping the quails, a piece of their chest has been removed immediately and were transferred to the laboratory. Myostatin gene expression measured by using RT-qPCR technique. In this method, 𝜷-actin gene was used as a house-keeping gene to normalize the gene expression data in the quantitative real time PCR. Results DL-methionine replacement did not significantly effect on Myostatin gene expression. Whereas, reduction in protein surface from 24% to 20 %, led to significantly increased expression of Myostatin (P<0.01). Conclusions The results indicated that DL methionine could be replaced with L methionine, and the appropriate level of protein was 24% in the Japanese quail diet.

https://jab.uk.ac.ir/article_2386_1bb6a85232fb240a0faadc21334fbcd5.pdf