Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 12 2 2020 08 22 Effects of sucrose and chitosan elicitors on expression of CYP79F1 gene and sulforaphane content in lepidium draba seedlings Effects of sucrose and chitosan elicitors on expression of CYP79F1 gene and sulforaphane content in lepidium draba seedlings 63 80 2673 10.22103/jab.2020.15354.1201 FA Ali Riahi-Madvar Biotechnology Dept., Institute of Science and High technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. of Iran Fereshteh Jadid Bonyad Former M.Sc. Student, Biotechnology Department, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran. Fatemeh Rezaee Former Ph.D. Student, Biology Department, Faculty of science, Ferdowsi University of Mashhad, Mashhad, Iran. Mahmood Maleki Assistant Professor, Biotechnology Department, Institute of Science and High technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman Mahshid Ghazizadeh Ahsaei Former M.Sc. Student, Biotechnology Department, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran. Journal Article 2020 03 17 Objective Glucoraphanine is an aliphatic glucosinolate, which largly found in Lepidium draba from Brassicaceae family. This glucosinolate convert to isothiocyanate, sulforaphane (SFN) in the presence of myrosinase which has different biological activities such as the antioxidant and anti bacterial properties, and also able to prevent proliferating of cancer cells. CYP79F1 is the first enzyme in the biosynthesis pathway of glucoraphanine. Materials and Methods In this study, L. draba seedlings wer grown for 7 days in the presence of different concentration of sucrose and chitosan (0, 25, 50, 100, 200 and 400 mg/L), in fully-random designs with three repetitions. After collection of the seedlings, SFN content in the arial parts of them were measured using HPLC apparatus. Furthermore, in the treated seedlings, the gene expression level of CYP79F1 were examined using the Real Time PCR technique. Results The results showed, that SFN content in the sucrose treated seedlings significantly increased compared to the control. While in the chitosan treated seedlings significant increase in SFN content was only seen at the 200 mg/L concentration. The results showed that the expression of CYP79 F1 was significantly increased in the treated seedlings with 50 mg/L sucrose and 50 and 100 mg/L chitosan compared to the control. Conclusions According to the results, it concluded that the mentioned elicitor's concentrations can stimulate aliphatic glucosinolate biosynthesis through expression of CYP79F1 gene. Based on the role of SFN as a valuable medicinal metabolit and also the role of CYP79F1 enzyme in the glucoraphanin biosynthesis, it suggested that the effects of others concentrations and time of treatment of the mentioned elicitors were analyzed on this plant. Objective Glucoraphanine is an aliphatic glucosinolate, which largly found in Lepidium draba from Brassicaceae family. This glucosinolate convert to isothiocyanate, sulforaphane (SFN) in the presence of myrosinase which has different biological activities such as the antioxidant and anti bacterial properties, and also able to prevent proliferating of cancer cells. CYP79F1 is the first enzyme in the biosynthesis pathway of glucoraphanine. Materials and Methods In this study, L. draba seedlings wer grown for 7 days in the presence of different concentration of sucrose and chitosan (0, 25, 50, 100, 200 and 400 mg/L), in fully-random designs with three repetitions. After collection of the seedlings, SFN content in the arial parts of them were measured using HPLC apparatus. Furthermore, in the treated seedlings, the gene expression level of CYP79F1 were examined using the Real Time PCR technique. Results The results showed, that SFN content in the sucrose treated seedlings significantly increased compared to the control. While in the chitosan treated seedlings significant increase in SFN content was only seen at the 200 mg/L concentration. The results showed that the expression of CYP79 F1 was significantly increased in the treated seedlings with 50 mg/L sucrose and 50 and 100 mg/L chitosan compared to the control. Conclusions According to the results, it concluded that the mentioned elicitor's concentrations can stimulate aliphatic glucosinolate biosynthesis through expression of CYP79F1 gene. Based on the role of SFN as a valuable medicinal metabolit and also the role of CYP79F1 enzyme in the glucoraphanin biosynthesis, it suggested that the effects of others concentrations and time of treatment of the mentioned elicitors were analyzed on this plant.

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