Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 16 3 2024 09 22 Assessment of lc and fas genes on flower organs in cherry tomato genetic background via introgression Assessment of lc and fas genes on flower organs in cherry tomato genetic background via introgression 23 42 4508 10.22103/jab.2024.22914.1550 FA Fatemeh Kakoeei Nejad Department of Genetics and Plant Production, Faculty of Agriculture, Shahid Bahonar University of Kerman,Kerman, Iran 0009-0006-8912-721X Mehdi Mohayeji Department of Genetics and Plant Production, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. 0000-0002-8499-609X Ali Akbar Maghsoudi Moud Department of Genetics and Plant Production, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. 0000-0002-2435-2110 Journal Article 2024 02 07 Objective Tomato (Solanum lycopersicum L.) is divided into two botanical varieties cerasiforme and lycopersicum. Many researchers believe that during domestication process, some changes in key genes governing flower properties in cherry tomato (S. lycopersicum var. cerasiforme) led to the development of the variety lycopersicum. The aim of this research was transferring lc and fas genes to the cherry tomato genetic background and assessment the effects of these two loci on flower characteristics in cherry tomato background. Materials and methods In this survey the genes lc and fas were transferred to a cherry tomato line p-33 via marker-assisted backcrossing. In each back cross generation, mutated alleles were chosen using specific molecular markers. Backcrossing was continued up to BC3F1. Afterward, the generation BC3F2 was produced and homozygote near-isogenic lines (NILs) were generated for assessment of genes lc and fas in p-33 genetic background. The flower traits including the number of petals, number of sepals, number of stamens, primary fruit weight and locule number were measured. Results The results showed that the effects of genes lc and fas on the trait's number of petals, sepals, and stamens were statistically significant at 5% level. While the effects of the mentioned genes on the rest of traits was significant at 1% level. The number of sepals, petals, and stamens in NILs-fas increased 31.28%, 36.79%, and 40.48% with respect to line p-33, respectively. Also, the number of locules increased in NILs-lc and NILs-fas with respect to line p-33 (37.88% and 47.63% respectively). Moreover, the primary fruit diameter showed increment in NILs-lc and NILs-fas respect to line p-33 (14.91% and 11.81% respectively). lc and fas loci had also a magnificent effect on flower size and showed incredible increase in NILs-lc and NILs-fas respect to line p-33. Conclusions The result of this study displayed that lc and fas genes have dramatic effect of in flower characteristics of tomato var. cerasiforme. The results also showed that the studied genes had key role in giving rise to S. lycopersicum var. lycopersicum form S. lycopersicum var. cerasiforme during tomato domestication. Objective Tomato (Solanum lycopersicum L.) is divided into two botanical varieties cerasiforme and lycopersicum. Many researchers believe that during domestication process, some changes in key genes governing flower properties in cherry tomato (S. lycopersicum var. cerasiforme) led to the development of the variety lycopersicum. The aim of this research was transferring lc and fas genes to the cherry tomato genetic background and assessment the effects of these two loci on flower characteristics in cherry tomato background. Materials and methods In this survey the genes lc and fas were transferred to a cherry tomato line p-33 via marker-assisted backcrossing. In each back cross generation, mutated alleles were chosen using specific molecular markers. Backcrossing was continued up to BC3F1. Afterward, the generation BC3F2 was produced and homozygote near-isogenic lines (NILs) were generated for assessment of genes lc and fas in p-33 genetic background. The flower traits including the number of petals, number of sepals, number of stamens, primary fruit weight and locule number were measured. Results The results showed that the effects of genes lc and fas on the trait's number of petals, sepals, and stamens were statistically significant at 5% level. While the effects of the mentioned genes on the rest of traits was significant at 1% level. The number of sepals, petals, and stamens in NILs-fas increased 31.28%, 36.79%, and 40.48% with respect to line p-33, respectively. Also, the number of locules increased in NILs-lc and NILs-fas with respect to line p-33 (37.88% and 47.63% respectively). Moreover, the primary fruit diameter showed increment in NILs-lc and NILs-fas respect to line p-33 (14.91% and 11.81% respectively). lc and fas loci had also a magnificent effect on flower size and showed incredible increase in NILs-lc and NILs-fas respect to line p-33. Conclusions The result of this study displayed that lc and fas genes have dramatic effect of in flower characteristics of tomato var. cerasiforme. The results also showed that the studied genes had key role in giving rise to S. lycopersicum var. lycopersicum form S. lycopersicum var. cerasiforme during tomato domestication.

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