Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 17 3 2025 08 23 Using Various Biotic and Abiotic Elicitors in Hazelnut Cell Suspension Cultures to Investigate the Expression of the 3-N-Debenzoyl-2-DeoxyTaxoln-Benzoyltransferase Gene in the Paclitaxel Biosynthesis Pathway Using Various Biotic and Abiotic Elicitors in Hazelnut Cell Suspension Cultures to Investigate the Expression of the 3-N-Debenzoyl-2-DeoxyTaxoln-Benzoyltransferase Gene in the Paclitaxel Biosynthesis Pathway 343 360 5012 10.22103/jab.2025.23315.1564 FA Raziyeh Bahrasmani Sardoo MS student of plant biotechnology, Department of Agricultural Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. Sara Alsadat Rahpeyma Department of Agricultural Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. Mehdi Mansouri Department of Agricultural Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. Journal Article 2024 04 29 Background<br />Paclitaxel (PC) is a naturally occurring chemotherapeutic medication used to treat various malignancies. Hazelnut (Corylus avellana L.) is currently a readily available and affordable Paclitaxel source. In this study, the potential effects of some biotic and abiotic elicitors were investigated on expression levels of the DBTNBT gene (3-N-debenzoyl-2-deoxytaxolN-benzoyltransferase) in cell suspension cultures of C. avellana. The DBTNB gene is one of the key genes in the downstream biosynthesis pathway of Paclitaxel.<br />Materials and methods<br />Hazelnut yellow friable calli taken from and often subcultured in MS media, supplemented with 2,4-D (2 mg/L) and BAP (0.2 mg/L), was suspended in liquid MS of the same composition. The elicitors methyl jasmonate (MeJA) (0, 100, and 200 (µL)), silver nitrate (0, 15, and 30 (mg/L)), or fungus extract (0, 25, 50, and 100 (mg/L)) were applied to the hazelnut cell cultures for 48 hours during the middle growth phase.<br />Results <br />The present study demonstrated the inductive effects of the elicitors on the expression of the DBTNBT gene. Compared to the control samples, in the C. avellana cell suspension culture, DBTNBT gene expression was affected positively by MeJA, and the most increase in DBTNBT gene expression (17.6-fold) was obtained from the treatment of 200 µL MeJA. The rate of gene expression rose considerably, up to 14 times greater than that of the control, when the concentration of AgNO3 was increased to 30 mg/L. Fungal extract affected DBTNBT gene expression; a suspension culture of C. avellana cells treated with 50 mg/L fungal extracts of C. globosum revealed a 4.75-fold increase in DBTNBT gene expression relative to the reference. However, 100 mg/L of C. globosum extracts reduced gene expression compared to the control.<br />Conclusions<br />MeJA had the highest degree of DBTNBT gene expression of any elicitor therapy used in this investigation, however all applied elicitation treatments were able to successfully increase the DBTNBT gene expression in hazelnut cell suspension cultures. Background<br />Paclitaxel (PC) is a naturally occurring chemotherapeutic medication used to treat various malignancies. Hazelnut (Corylus avellana L.) is currently a readily available and affordable Paclitaxel source. In this study, the potential effects of some biotic and abiotic elicitors were investigated on expression levels of the DBTNBT gene (3-N-debenzoyl-2-deoxytaxolN-benzoyltransferase) in cell suspension cultures of C. avellana. The DBTNB gene is one of the key genes in the downstream biosynthesis pathway of Paclitaxel.<br />Materials and methods<br />Hazelnut yellow friable calli taken from and often subcultured in MS media, supplemented with 2,4-D (2 mg/L) and BAP (0.2 mg/L), was suspended in liquid MS of the same composition. The elicitors methyl jasmonate (MeJA) (0, 100, and 200 (µL)), silver nitrate (0, 15, and 30 (mg/L)), or fungus extract (0, 25, 50, and 100 (mg/L)) were applied to the hazelnut cell cultures for 48 hours during the middle growth phase.<br />Results <br />The present study demonstrated the inductive effects of the elicitors on the expression of the DBTNBT gene. Compared to the control samples, in the C. avellana cell suspension culture, DBTNBT gene expression was affected positively by MeJA, and the most increase in DBTNBT gene expression (17.6-fold) was obtained from the treatment of 200 µL MeJA. The rate of gene expression rose considerably, up to 14 times greater than that of the control, when the concentration of AgNO3 was increased to 30 mg/L. Fungal extract affected DBTNBT gene expression; a suspension culture of C. avellana cells treated with 50 mg/L fungal extracts of C. globosum revealed a 4.75-fold increase in DBTNBT gene expression relative to the reference. However, 100 mg/L of C. globosum extracts reduced gene expression compared to the control.<br />Conclusions<br />MeJA had the highest degree of DBTNBT gene expression of any elicitor therapy used in this investigation, however all applied elicitation treatments were able to successfully increase the DBTNBT gene expression in hazelnut cell suspension cultures. https://jab.uk.ac.ir/article_5012_218c37084dbeb28702e59f91cfd85048.pdf