Shahid Bahonar University of Kerman and Iranian Biotechnology Society Agricultural Biotechnology Journal 2228-6705 18 4 2026 12 22 Assessing the feasibility of marine animal tissue culture for lab-grown seafood in maritime systems Assessing the feasibility of marine animal tissue culture for lab-grown seafood in maritime systems 539 552 5649 10.22103/jab.2026.25258.1711 FA Abhishek Kumar Pandey Assistant Professor, Kalinga University, Naya Raipur, Chhattisgarh, India. 0009-0005-3712-5629 Deepa Biswas Assistant Professor, Kalinga University, Naya Raipur, Chhattisgarh, India 0009-0009-6875-7134 Journal Article 2025 05 13 ObjectiveThe current study attempts to explore the viability of using marine animal TC to generate seafood in laboratories. The current work focuses on exploring the biological and technical constraints associated with the large-scale culturing of marine organisms, especially in generating CBS. The current research is expected to shed light on the potential of CBS as a sustainable alternative in the seafood sector.Materials and MethodsTissue cultures of marine organisms, which include fish and crustaceans, have been utilized to assess the viability of producing CBS on a large scale. This includes the use of cell line generation, the creation of serum-free media, and the design of bioreactors. The integration of genetic engineering with conventional AC techniques was used to establish an ideal growth environment for the cells. Several culture conditions were assessed, including resistance to hypoxia, regulation of pH, and adaptation to low temperatures.ResultsThis experiment indicated that marine animal cells, especially those from fish and crustaceans’ muscles, were better adapted to hypoxia and low temperatures than terrestrial cells. This is beneficial for CBS fabrication since it facilitates effective growth and differentiation of cells in the bioreactor. Furthermore, cell cultures derived from fish tissues were capable of forming tissue-like constructs on biodegradable scaffolds, which could pave the way for growing seafood in the laboratory. However, there were challenges associated with the creation of serum-free medium and bioreactor design. ConclusionIn conclusion, marine animal tissue culture can potentially be used as a viable technology for growing seafood sustainably. While there are a myriad of physiological benefits associated with using marine cells in the manufacture of cultured seafood, much remains to be accomplished in addressing some technical challenges in this area, including the development of serum-free medium, culturing of cell lines, and bioreactor design. Solving these problems could usher in a paradigm shift in the industry, offering a sustainable solution to the burgeoning global demand for seafood. ObjectiveThe current study attempts to explore the viability of using marine animal TC to generate seafood in laboratories. The current work focuses on exploring the biological and technical constraints associated with the large-scale culturing of marine organisms, especially in generating CBS. The current research is expected to shed light on the potential of CBS as a sustainable alternative in the seafood sector.Materials and MethodsTissue cultures of marine organisms, which include fish and crustaceans, have been utilized to assess the viability of producing CBS on a large scale. This includes the use of cell line generation, the creation of serum-free media, and the design of bioreactors. The integration of genetic engineering with conventional AC techniques was used to establish an ideal growth environment for the cells. Several culture conditions were assessed, including resistance to hypoxia, regulation of pH, and adaptation to low temperatures.ResultsThis experiment indicated that marine animal cells, especially those from fish and crustaceans’ muscles, were better adapted to hypoxia and low temperatures than terrestrial cells. This is beneficial for CBS fabrication since it facilitates effective growth and differentiation of cells in the bioreactor. Furthermore, cell cultures derived from fish tissues were capable of forming tissue-like constructs on biodegradable scaffolds, which could pave the way for growing seafood in the laboratory. However, there were challenges associated with the creation of serum-free medium and bioreactor design. ConclusionIn conclusion, marine animal tissue culture can potentially be used as a viable technology for growing seafood sustainably. While there are a myriad of physiological benefits associated with using marine cells in the manufacture of cultured seafood, much remains to be accomplished in addressing some technical challenges in this area, including the development of serum-free medium, culturing of cell lines, and bioreactor design. Solving these problems could usher in a paradigm shift in the industry, offering a sustainable solution to the burgeoning global demand for seafood. https://jab.uk.ac.ir/article_5649_337cd73a31464dd4adfc3c5dbc356cd0.pdf