ساخت واکسن پلی اپی توپی بر علیه بیماری نیوکاسل بوسیله طراحی ایمونوانفورماتیکی پروتئین های ایمنی زا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی دانشگاه جیرفت

2 شیلات- دانشده علوم دامی و شیلات - دانشگاه کشاورزی و منابع طبیعی ساری

3 بخش علوم دامی دانشکده کشاورزی دانشگاه جیرفت

چکیده

هدف: استفاده از تکنیک‌های زیست‌شناسی مولکولی برای تولید واکسن‌های جدید علیه سویه‌های مختلف ویروس بیماری نیوکاسل (NDV)  موضوع گزارش‌های تحقیقاتی اخیر بوده است. توسعه تکنیک‌های بهبودیافته برای تعیین توالی ژنوم منجر به شناسایی مکانیسم‌های محافظتی و شناسایی آنتی‌ژن‌های احتمالی کاندید شده است؛ تحقیق حاضر با هدف طراحی یک اپی توپ نوترکیب در برابر عوامل اتصال در ویروس نیوکاسل در پرندگان انجام شده است.
مواد و روش‌ها: در این تحقیق برای ساختن واکسن نوترکیب از روش‌های بیوانفورماتیکی استفاده شده است تا با استفاده از آنتی‌ژن‌های ضد این ویروس، آنتی‌ژن‌های HN و F این بیماری را کنترل و از بین ببریم. برای اتصال این اپی توپ‌ها، از لینکرهای انعطاف‌پذیر مانند AAY  و  KK به‌عنوان پیونددهنده‌های ساختار انتخاب شدند. این ساختار حاوی 309 اسیدآمینه است. فاکتورهای مهم بیولوژیکی این واکسن نوترکیب مانند خصوصیات فیزیکی-شیمیایی، ساختارهای مختلف، پایداری، اختلال پروتئین ذاتی، حلالیت و حساسیت‌زایی این ساختار واکسن با استفاده از تجزیه‌وتحلیل سیستم ایمنی بدن ارزیابی شد.
نتایج: تجزیه ‌و تحلیل‌های مختلف پایداری این ساختار را تأیید کرد و اپی توپ‌های پیش‌بینی‌شده در واکسن نوترکیب پتانسیل بالایی را برای القای پاسخ ایمنی سلول Bهای و T نشان داد؛ بنابراین، تجزیه‌وتحلیل سیستم ایمنی نشان داد که واکسن چند اپی توپی می‌تواند به ‌درستی پاسخ‌های ایمنی سلول T و B را تحریک کند و به طور بالقوه می‌تواند برای برنامه‌های پیشگیری یا کنترل استفاده شود. از نتایج این مطالعه می‌توان برای کنترل و از بین بردن بیماری نیوکاسل در آینده پس از تأیید اثربخشی آن با استفاده از روش‌های ایمونولوژیک تجربی استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Construction of a polyepitope vaccine against Newcastle disease by immunoinformatics design of immunogenic proteins

نویسندگان [English]

  • Zahra Rodbari 1
  • Abdolvahab Ebrahimpour Gorji 2
  • Arsalan Brazandeh 3
1 Zahra Roudbari, Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
2 Sari University of Agricultural Sciences and Natural Resources, Sari, Iran Department of Animal Science and fishery
3 Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
چکیده [English]

Objective
Application of molecular biology techniques to the production of new vaccines against different strains of the Newcastle disease virus (NDV) has been the subject of recent research reports. Development of improved techniques for genome sequencing has led to the recognition of protective mechanisms and the identification of possible candidate antigens. The present research aimed to design a recombinant chimeric immunogen against binding agents in Newcastle virus in birds.
 
Materials and methods
Therefore, we conducted this investigation to make a recombinant vaccine in silico in order to control and eliminate this disease using anti-leptospirosis epitopes, F, and HN antigens. To bind these epitopes, KK, as AAY linkers, were selected to bind the structure. This structure contained 309 amino acids. The important biological factors of this recombinant vaccine, such as physicochemical properties, different structures, stability, intrinsic protein disorder, solubility, and allergenicity of this vaccine structure were evaluated using immune system analysis.
 
Results
Various analyses confirmed the stability of this structure, and the epitopes predicted in the chimeric vaccine showed a high potential for inducing the immune response of B cell and T cell. Thus, immune system analysis revealed that the modeled multi-epitope vaccine could properly stimulate T and B cell immune responses and could potentially be utilized for prophylactic or control applications.
 
Conclusion
The results of this study could be employed to control and eliminate leptospirosis in the future after confirming its effectiveness by experimental immunological assays

کلیدواژه‌ها [English]

  • Newcastle
  • Vaccine design
  • F and HN
  • Multi- epitope
 
 
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