تجزیه و تحلیل ژنتیکی و فیلوژنتیکی ناحیه سیتوکروم b در جبیر ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه علوم دامی و پژوهشکده زیست فناوری، دانشگاه فردوسی مشهد

2 دانشجوی مقطع دکتری، گروه علوم دامی، دانشگاه فردوسی مشهد

چکیده

جبیر از زیرخانواده بزهای کوهی (Antilopinae) و جنس آهو (Gazella) می باشد که عمدتاً در نواحی کویر مرکزی و جنوبی ایران زیست می کند. به لحاظ تعیین گونه همواره چالش های زیادی در بین محققین متوجه این حیوان بوده است. هدف از اجرای این مطالعه بررسی ناحیه سیتوکرم b از DNA میتوکندریایی جبیر جهت انجام مطالعات ژنتیکی و فیلوژنتیکی بود. تعداد 5 نمونه بیولوژیک از جبیرهای پناهگاه حیات وحش شیراحمد سبزوار در طول سالهای 86 تا 88 جمع آوری شدند. پس از استخراج DNA از نمونه ها، تکثیر ناحیه سیتوکروم b از DNA میتوکندری به طول 1140 جفت باز به کمک جفت پرایمرهای L14724 و H15915 صورت گرفت. محصولات PCR به کمک دستگاه ABI 3130 به روش سانگر توالی یابی شدند. این توالی ها با 190 توالی موجود از جنس آهو که بر اساس گونه تقسیم بندی شده بودند از لحاظ میزان هم پوشانی و فاصله ژنتیکی به کمک برنامه Bioedit 7 و MEGA5 مقایسه شده و درخت فیلوژنتیک آنها رسم شد. نتایج این تحقیق نشان داد که در بین گونه های مختلف آهو، جبیر کمترین فاصله ژنتیکی، که معادل 0.003 است را با گونه G. bennetti دارد، در حالی که بیشترین فاصله ژنتیکی جبیر با G. dorcas و یا تاکسون خواهری اش G. saudiya می باشد که به ترتیب برابر 0.073 و 0.074 است. این امر می تواند موید تعلق جبیر به گونه G. bennetti باشد

کلیدواژه‌ها

عنوان مقاله [English]

Genetic and phylogenetic analysis of cytochrome b region in Jebeer of Iran

نویسندگان [English]

  • Mohammadreza Nassiri 1
  • Morteza Mahdavi 2
چکیده [English]

Jebeer is belonging to subfamily of mountain goats (Antilopinae), genus of Gazelle (Gazella) and found mainly in central and southern desert areas of Iran. There are many challenges between researchers about determining Jebeer species. Whereas determining Jebeer species is first step in protecting of them we perform this research. In this regard 5 biological samples from Shirahmad’s wild life refuge of Sabzevar were collected and cytochrome b region of mitochondrial DNA have been amplified with L14724 and H15915 primers. Resulting fragments were sequenced with ABI 3130 automated device with Sanger method. These sequences compared with 190 sequences from Gazella genus that classified according to species with regard to homology and genetic distances, then phylogenetic tree have been drawn. Results show that among all Gazelle sp. Jebeer have least distance equal to 0.003 with G. bennetti  and most distance with G. dorca and his sister taxon G. saudiya that is equal to 0.073 and 0.074 respectively.  Thereby can conclude that Jebeer is belonging to G. bennetti species not G. dorcas

کلیدواژه‌ها [English]

  • Jebeer
  • Cytochrome b
  • Mitochondrial DNA
  • Gazella bennetti

اصل مقاله

Jebeer, Cytochrome b, Mitochondrial DNA, Gazella bennetti.

 

مراجع

Arctander P, Kat PW, Aman RA, Siegismund HR (1996). Extreme genetic differences among populations of Gazella granti, Grant’s gazelle, in Kenya. Heredity 76: 465-475.
Beja-Pereira A, Zeyl E, Ouragh L, Nagash H, Ferrand N, Taberlet P, Luikart G (2004). Twenty polymorphic microsatellites in two of North Africa's most threatened ungulates: Gazella dorcas and Ammotragus lervia (Bovidae; Artiodactyla). Molecular Ecology Notes 4: 452-455.
Bellagamba F, Moretti VM, Comincini S, Valfare F (2001). Identification of species in animal feedstuffs by polymerase chain reaction restriction fragment length polymorphism analysis of mitochondrial DNA. Journal of Agricultural and Food Chemistry 49: 3775-3781.
Blaine G (1914). Catalogue of the ungulate mammals in the British Museum: vol 3. British Museum (Natural History), London.
Blanford WT (1873). Note on the gazelles of lndia and Persia, with description of a  new species. Proceedings of the Zoological Society of London, London, pp. 313-319.
Bohra HC, P. GS, Ghosh PK, Prakash I (1992). Studies on ethology and ecophysiology of Antelopes of the Indian Desert. Annals of Arid Zone 31: 83-96.
Bruford M, Bradley D, Luikart G (2003). DNA markers reveal the complexity of livestock domestication. Nature Reviews Genetics 3: 900-910.
Burgener M, Hübner P (1998). Mitochondrial DNA enrichment for species identification and evolutionary analysis. Z Lebensm Unters Forsch A 207: 261-263.
Collura RV, Stewart CB (1995). Insertions and duplications of mtDNA in the nuclear genomes of Old World monkeys and hominoids. Nature 378: 485-489.
Corbet GB (1978). The mammals of the palaearctic region: A taxonomic review. British Museum (Natural History) and Cornell University Press, London and Ithaca.
Ellerman JR, Morrison-Scott TCS (1951). Checklist  of Palaearctic  and  Indian  mammals, 1758  - 1947, 2 edn. British Museum (Natural History), London.
Etemad I (1985). Mamals of Iran, vol 2. Iran wildlife center (In Farsi).
Ewing B, Green P (1998). Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities. Genome Research 8: 186-194.
Eyre-walker A, Awadalla P (2001). Does human mtDNA recombine? Journal of Molecular Evolution 53: 430-435.
Firouz E (2005). The Complete fauna of Iran. I.B.Tauris, London.
Funk DJ, Omland KE (2003). Species-level Paraphyly and Polyphyly: Frequency, Causes, and Consequences, with Insights from Animal Mitochondrial DNA. Annual Review of Ecology, Evolution, and Systematics 34: 397-423.
Furley CW, Tichy H, Verpmann HP (1988). Systematics and chromosomes of the Indian gazelle, Gazella bennetti (Sykes, 1831). Zeitschrift für Säugetierkunde 53: 48-54.
Groves CP (1985). An introduction to the gazelles. Chinkara 1: 4-16.
Groves CP (1993). The chinkara (Gazella Bennetti) in Iran, With The Description Of Two New Subspecies. Journal of science of I. R. Iran 4: 166-178.
Groves CP, Harrison DL (1967). the taxonomy of the gazelles (genus Gazella) of Arabia. Journal of Zoology 152: 381-387.
Hammond RL, Macasero W, Flores B, Mohammed OB, Wacher T, Bruford MW (2001). Phylogenetic reanalysis of the saudi gazelle and its implications for conservation. Conservation Biology 15: 1123-1133.
Hebert PDN, Cywinska A, deWaard JR (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society Biological Sciences 270: 313- 321.
Huebinger RM, Maar TWJD, Woodruff LH, Pomp D, Louis Jr EE (2006). Characterization of eight microsatellite loci in Grant's gazelle (Gazella granti). Molecular Ecology Notes 6: 1150-1151.
Hundertmark KJ (2005). Research Summary at KKWRC. Thumamah, Saudi Arabia: King Khalid Wildlife Research Centre, Unpublished.
Irwin DM, Kocher TD, Wilson AC (1991). Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolution 32: 128-144.
Karami M (1993). Multivariate analysis and descriptive statistics of Persian gazelle skull from faroor island of Iran. Iranian Journal of Natural Resources 46: 95 (In Farsi).
Koch H (2010). Combining morphology and DNA barcoding resolves the taxonomy of Western Malagasy Liotrigona Moure. African Invertebrates 51: 413-421.
Kocher TD, Thomas WK, Meyer A, Edwards SV, Paabo S, Villablanca FX, Wilson AC ( 1989). Dynamics of Mitochondrial DNA Evolution in Animals: Amplification and Sequencing with Conserved Primers. Proceedings of the National Academy of Sciences USA 86: 6196-6200.
Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH (2005). Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences USA 102: 8369-8374.
Lerp H, Wronski T, Pfenninger M, Plath M (2011). A phylog eographic framework for the conservationof Sahara n and Arabian Dorcas gazelles (Artiodactyla: Bovidae ). Organisms Diversity & Evolution 11: 317 -329.
Lydekker R (1908). The gazelles of Seistan. The Field 111:499.
Lydekker R (1911). An African rhinoceros, klipspringer, and gazelle. Proceedings of the Zoological Society of Londoon 111: 958-962.
Prater SH (1971). The book of Indian animals. Bombay Natural History Society, Bombay.
Rebholz WER, Harley EH (1997). Cytochrome b  Sequences from the Endangered Saudi Gazelle (Gazella Saudiya) Suggest Hybridization with Chinkara (G. bennetti). Conservation Biology 11: 251-255.
Rebholz W, Harley E (1999). Phylogenetic relationships in the bovid subfamily Antilopinae based on mitochondrial DNA sequences. Journal of Molecular Phylogenetics and Evolution 12: 87-94.
Seberg O, Petersen G (2009). How many loci does it take to dna barcode a crocus? PLoS One 4: e4598.
Tamura K, Nei M, Kumar S (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101: 11030-11035.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739.
Vassart M, Granjon L, Greth A, Catzeflis FM (1994). Genetic relationships of some Gazella species: an allozyme survey. Zeitschrift fur saugetierkunde 59: 236-245.
 Wacher T, Wronski T, Hammond RL, Winney B, Blacket MJ, Hundertmark KJ, Mohammed OB, Ome SA, Macasero W, Lerp H, Plath M, Bleidorn C (2011). Phylogenetic analysis of mitochondrial DNA sequences reveals polyphyly in the Goitred gazelle (Gazella subgutturosa). Conservation Genetics 12: 827-831.
Ziaie H (2008). A field guide to the mammals of Iran, 2 edn. Iran wildlife center.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
مجله بیوتکنولوژی کشاورزی
دوره 3، شماره 1 - شماره پیاپی 1
سال1 شماره 3 بهارو تابستان 1390
خرداد 1390
صفحه 91-104

فایل ها

هم رسانی

ارجاع به این مقاله

آمار