Isolation and study of morphology and phylogeny of arbuscular mycorrhizal fungi coexisting with the roots of some medicinal plants in Kerman province

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.

2 Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad , Iran

3 Department of Plant Protection. Faculty of Agriculture, Vali-e-Asr University , Rafsanjan, Iran

4 Department of Plant Protection. Faculty of Agriculture, Lorestan University, Khorramabad , Iran

Abstract

Objective
Plants that have a symbiotic relationship with arbuscular mycorrhizal fungi (AMF) compared with non-mycorrhizal plants have a higher ability to resist against various biotic and abiotic environmental stresses such as drought, salinity, pests, and plant diseases. This study aim is studying different species of AMFs in Kerman province. The identification of these microorganisms is to provide the basis for further studies on the use of these microorganisms in germination, growth, and proliferation of medicinal plants.
Methods
To molecular and morphological identification of arbuscular mycorrhizal fungi, sampling was done from the rhizosphere of medicinal plants in some areas of Kerman province. Arbuscular mycorrhizal fungi spores were isolated from the sampled soils by the wet sieve method. The grouping was done based on spore microscopic slides and morphological characteristics such as spore color, shape, surface ornamentation, size, wall structure and spore characteristics in water. DNA was extracted from single spores. A Part of ribosomal DNA was amplified by nested PCR using SSUmaf and LSUmAr primers in the first step, SSUmCf, and LSUmBr in the second step. After horizontal agarose gel electrophoresis and see the related band the PCR product was sent for sequencing. Then sequencing results were analyzed using BLAST, Gene Runner and Clustal Omega Softwares, and the phylogenetic tree was plotted using MEGA 7 software.
Results
According to morphological and molecular studies, Viscospora viscosaSeptoglomus deserticolaFunneliformis caledoniumFunneliformis mosseae, and Diversispora spurca were identified. Finally, the existence and manner of species identity were investigated in the phylogeny tree.
Conclusion
Since the morphological features used in the identification and classification of mycorrhizal fungi are limited, molecular methods can be used for safer classification, and in this way, Recognize differences of Similar species morphologically and similarity of different species in appearance.

Keywords


احیایی احمد رضا؛ رضوانی مقدم پرویز؛ امیری ده احمدی سید رضا (1388) بررسی تاثیر تنش خشکی بر برخی شاخص های مورفولوژیکی سه گیاه دارویی خارمریم ، همیشه بهار و سیاهدانه در شرایط گلخانه. اولین همایش ملی تنش های محیطی در علوم کشاورزی
امیدبیگی رضا (1384) تولید و فرآوری گیاهان دارویی. ویرایش دوم. انتشارات آستان قدس رضوی. 438.
ثابت جهرمی مهدی؛ صالحی جوزانی غلامرضا؛ حسینی ملیحه؛ خیام نکویی سید مجتبی؛ اکبری والا سپیده (1391) جداسازی و شناسایی قارچ‌های میکوریز آربسکولار بومی در خاک‌های مناطق خشک زراعی ایران با استفاده از روش PCR آشیانه‌ای. زیست فناوری تربیت مدرس 3. 1-13.
زنگنه سیما؛ شیروانی علی بخش؛ علیان یعقوب؛ نجفی‌نیا موسی؛ کرم‌پور فرزاد؛ قلعه‌دزدانی حجت الله (1384) معرفی گونه‌های جدیدی از قارچ‌های آربسکولار-میکوریزا از ریزوسفر مرکبات ایران. رستنی‌ها جلد 6، 77-8.
صالحی‎‌جوزانی غلامرضا؛ اکبری‌والا سپیده؛ ثابت‌جهرمی مهدی؛ مرسلی حسن (1390) جداسازی و شناسایی قارچ‌های میکوریز آربوسکولار غالب در ریزوسفر گندم، جو و علف‌های هرز برخی مناطق زراعی شور ایران. مجله علمی-پژوهشی زیست‌فناوری گیاهان زراعی. جلد 1، 75-61.
صدروی مهدی (1381) معرفی پنج گونه گلوموس از قارچ‌های میکوریز آربوسکولار ایران. علوم کشاورزی و منابع طبیعی ایران. جلد 9. 30-15.
صدروی مهدی؛ (1385) قارچ‌های میکوریز آربوسکولار مزارع گندم در استان گلستان. مجله رستنی‌ها. جلد 7، 140-129.
فدایی الهام؛ پرویزی یحیی؛ گردکانه محمد؛ خان احمدی معصومه (1397) تأثیر القای سویه های قارچ میکوریزا Glomus intraradiceae Glomus mosseae و فسفر بررشد و ترکیبات فیتوشیمیایی بادرشبو(Dracocephalum moldavica L.) تحت شرایط تنش خشکی. فصلنامه گیاهان دارویی 17، 117-130.
 
 

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