تجزیه و تحلیل ژنتیکی و فیلوژنتیکی ناحیه سیتوکروم b در جبیر ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه علوم دامی و پژوهشکده زیست فناوری، دانشگاه فردوسی مشهد

2 دانشجوی مقطع دکتری، گروه علوم دامی، دانشگاه فردوسی مشهد

چکیده

جبیر از زیرخانواده بزهای کوهی (Antilopinae) و جنس آهو (Gazella) می باشد که عمدتاً در نواحی کویر مرکزی و جنوبی ایران زیست می کند. به لحاظ تعیین گونه همواره چالش های زیادی در بین محققین متوجه این حیوان بوده است. هدف از اجرای این مطالعه بررسی ناحیه سیتوکرم b از DNA میتوکندریایی جبیر جهت انجام مطالعات ژنتیکی و فیلوژنتیکی بود. تعداد 5 نمونه بیولوژیک از جبیرهای پناهگاه حیات وحش شیراحمد سبزوار در طول سالهای 86 تا 88 جمع آوری شدند. پس از استخراج DNA از نمونه ها، تکثیر ناحیه سیتوکروم b از DNA میتوکندری به طول 1140 جفت باز به کمک جفت پرایمرهای L14724 و H15915 صورت گرفت. محصولات PCR به کمک دستگاه ABI 3130 به روش سانگر توالی یابی شدند. این توالی ها با 190 توالی موجود از جنس آهو که بر اساس گونه تقسیم بندی شده بودند از لحاظ میزان هم پوشانی و فاصله ژنتیکی به کمک برنامه Bioedit 7 و MEGA5 مقایسه شده و درخت فیلوژنتیک آنها رسم شد. نتایج این تحقیق نشان داد که در بین گونه های مختلف آهو، جبیر کمترین فاصله ژنتیکی، که معادل 0.003 است را با گونه G. bennetti دارد، در حالی که بیشترین فاصله ژنتیکی جبیر با G. dorcas و یا تاکسون خواهری اش G. saudiya می باشد که به ترتیب برابر 0.073 و 0.074 است. این امر می تواند موید تعلق جبیر به گونه G. bennetti باشد

کلیدواژه‌ها

عنوان مقاله [English]

Genetic and phylogenetic analysis of cytochrome b region in Jebeer of Iran

نویسندگان [English]

  • Mohammadreza Nassiri 1
  • Morteza Mahdavi 2
1
2
چکیده [English]

Jebeer is belonging to subfamily of mountain goats (Antilopinae), genus of Gazelle (Gazella) and found mainly in central and southern desert areas of Iran. There are many challenges between researchers about determining Jebeer species. Whereas determining Jebeer species is first step in protecting of them we perform this research. In this regard 5 biological samples from Shirahmad’s wild life refuge of Sabzevar were collected and cytochrome b region of mitochondrial DNA have been amplified with L14724 and H15915 primers. Resulting fragments were sequenced with ABI 3130 automated device with Sanger method. These sequences compared with 190 sequences from Gazella genus that classified according to species with regard to homology and genetic distances, then phylogenetic tree have been drawn. Results show that among all Gazelle sp. Jebeer have least distance equal to 0.003 with G. bennetti  and most distance with G. dorca and his sister taxon G. saudiya that is equal to 0.073 and 0.074 respectively.  Thereby can conclude that Jebeer is belonging to G. bennetti species not G. dorcas

کلیدواژه‌ها [English]

  • Jebeer
  • Cytochrome b
  • Mitochondrial DNA
  • Gazella bennetti

اصل مقاله

Jebeer, Cytochrome b, Mitochondrial DNA, Gazella bennetti.

 

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مجله بیوتکنولوژی کشاورزی
دوره 3، شماره 1 - شماره پیاپی 1
سال1 شماره 3 بهارو تابستان 1390
خرداد 1390
صفحه 91-104

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