اثر میلورم بر بیان ژن GBP4L در بافت طحال جوجه‌های گوشتی سویه راس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد بخش علوم دامی ، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

2 دانشیار بخش علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، ایران

3 استادیار، دانشکده کویرشناسی، دانشگاه سمنان، سمنان، ایران.

4 بخش علوم دامی، دانشگاه ملی کشاورزی بیلا تسرکوا، بیلا تسرکوا، اکراین.

5 دانشگاه ملی کشاورزی بیلا تسرکوا، بیلا تسرکوا، اوکراین

6 دانشگاه ملی کشاورزی سومی، سومی، اوکراین

7 استادیار، دانشگاه ملی علوم محیطی و زیستی اوکراین، اوکراین

8 دانشگاه کشاورزی دولتی پولتاوا، اوکراین.

9 دانشیار، دانشگاه کشاورزی ایالتی پولتاوا، اوکراین

چکیده

هدف: کرم میلورم (Tenebrio molitor) می‌تواند هم به عنوان مکمل پروتئینی و هم به عنوان یک پری‌بیوتیک در جیره طیور استفاده شود. نشان داده شده که استفاده از آن هم فواید زیست محیطی دارد و هم باعث کاهش هزینه های تولید در مرغداری ها شده و سلامت طیور را افزایش می‌دهد. لذا، هدف این پژوهش اندازه گیری بیان نسبی ژن GBP4L در طحال جوجه‌های گوشتی سویه راس تحت اثر افزودن میلورم به جیره بود.
مواد و روش‌ها:
برای انجام این آزمایش 20 قطعه جوجه گوشتی در قالب طرح کاملاً تصادفی با 2 گروه، و 10 قطعه جوجه در هر گروه تحت تغذیه با میلورم به‌صورت افزودنی مطالعه شدند و هنگام کشتار از بافت طحال آنها نمونه‌برداری شد. سپس RNA کل با استفاده از کیت استخراج Total RNA استخراج شد. پس از استخراج و تخلیص RNA، کمیت و کیفیت RNA استخراج‌شده با الکتروفورز روی ژل آگارز و روش نانودراپ مورد بررسی قرار گرفت. از روی RNA کل cDNA ساخته شد. برای ارزیابی بیان نسبی ژن هدف (GBP4L) و ژن مرجع (GAPDH) از روش Real-time PCR استفاده شد و نتایج به‌وسیله نرم‌افزارهای Excel و Prism بررسی شد.

نتایج: در مطالعه حاضر، RNA کل استخراج‌شده دارای باندهای rRNA 18S و rRNA 28S واضح و کاملاً سالم بود. نتایج نشان‌دهنده RNA با غلظت معقول و فاقد آلودگی پروتئینی و الکلی بودند. استاندارد بودن منحنی‌های تکثیر ژن و منحنی‌های ذوب و وجود تک باند در نتایج حاصل از ژل الکتروفورز نشان‌دهنده اختصاصی عمل کردن واکنش‌ها بود. نتایج بیان ژن نشان داد که افزودن میلورم به جیره غذایی جوجه گوشتی تأثیر معنی‌داری (P < 0.05) بر افزایش بیان ژن GBP4L در بافت طحال در مقایسه با گروه کنترل داشت.
نتیجه‌گیری:
بر اساس یافته‌های این پژوهش و مقایسه با نتایج سایر پژوهش‌ها می‌توان نتیجه گرفت که با افزودن میلورم به جیره جوجه‌های گوشتی، می‌توان بیان نسبی GBP4L را در بافت طحال تغییر داد و با این تغییر باعث بهبود سیستم ایمنی جوجه های گوشتی شد و آن‌ها را در مقابل استرس گرمایی مقاوم تر نمود. لذا، می توان پیشنهاد داد که GBP4L پتانسیل این را دارد که به عنوان یک مداخله کننده مهم در بهبود سیستم ایمنی نقش ایفا کند و راه را برای انجام پژوهش‌های جدید با نمونه‌های بیشتر و با در نظر گرفتن شرایط فیزیولوژیکی و محیطی متفاوت هموار سازد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Mealworm on GBP4L Gene Expression in the Spleen Tissue of Ross Broiler Chickens

نویسندگان [English]

  • Mohammadreza Mohammadabadi 1
  • Mohsen Afsharmanesh 2
  • Amin Khezri 2
  • Hamid Kheyrodin 3
  • Olena Babenko Ivanivna 4
  • Oleksandr Borshch 5
  • Oleksandr Kalashnyk 6
  • Оleksandr Nechyporenko 6
  • Volodymyr Afanasenko 7
  • Viktor Slynko 8
  • Svitlana Usenko 9
1 Professor, Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
2 Associate Professor, Animal Science Department, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
3 Assistant Professor, Semnan University, Semnan, Iran.
4 Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine.
5 Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
6 Sumy National Agrarian University, Sumy, Ukraine
7 National University of Life and Environmental Sciences of Ukraine, Ukraine.
8 Poltava State Agrarian University, Ukraine.
9 Associate Professor, Poltava State Agrarian University, Ukraine.
چکیده [English]

Objective
The mealworm (Tenebrio molitor) can be used as both a protein supplement and a prebiotic in poultry diets. It has been shown that its use not only provides environmental benefits and reduces production costs in poultry farms but also improves poultry health. Therefore, the aim of this study was to measure the relative expression of the GBP4L gene in the spleen tissue of Ross broiler chickens under the effect of dietary mealworm supplementation.
Materials and methods
For this experiment, 20 broiler chicks were randomly divided into two groups of 10 birds each and fed with mealworm as a dietary additive. At the time of slaughter, spleen tissue samples were collected. Total RNA was extracted using a total RNA extraction kit. After RNA extraction and purification, the quantity and quality of the RNA were evaluated using agarose gel electrophoresis and NanoDrop analysis. Complementary DNA (cDNA) was synthesized from the total RNA. To assess the relative expression of the target gene (GBP4L) and the reference gene (GAPDH), Real-Time PCR was performed. Data analysis was carried out using Excel and Prism software.
Results
The extracted total RNA showed clear and intact 18S and 28S rRNA bands. The RNA exhibited reasonable concentration and was free from protein and alcohol contamination. The amplification and melting curves were standard, and the presence of single bands in gel electrophoresis confirmed the specificity of the reactions. Gene expression analysis indicated that the addition of mealworm to the broiler diet had a significant effect (P < 0.05) on increasing GBP4L gene expression in spleen tissue compared to the control group.
Conclusions
Based on the findings of this study and comparison with previous research, it can be concluded that dietary supplementation with mealworm can alter the relative expression of GBP4L in the spleen tissue of broiler chickens. This modulation may enhance immune function and increase resistance to heat stress in broilers. Therefore, GBP4L may serve as a key modulator in improving immune responses, paving the way for further research with larger sample sizes and under diverse physiological and environmental conditions.

کلیدواژه‌ها [English]

  • Tenebrio molitor
  • GBP4L gene
  • broiler chickens
  • immune response
  • heat stress

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مجله بیوتکنولوژی کشاورزی
دوره 17، شماره 2
اردیبهشت 1404
صفحه 343-360

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