Study of Antioxidant Activity of Bacterial Endophyte Extracts of Ferula gummosa boiss

Document Type : Research Paper

Authors

1 Biotechnology group, Bu Ali Sina University

2 Assistant Prof., Department of Biotechnology, Bu-Ali Sina University, Hamadan, Iran

3 Plant breeding department, Bu Ali Sina University

Abstract

Objective
Endophytes are known as a potential source of active natural compounds for use in medicine, agriculture, and industry. Medicinal plants are valuable sources for the study of endophytes. One of the most important medicinal and industrial plants in Iran is the Barije; Ferula gummosa.boiss, whose endophytes have not been studied yet.
 
Materials and methods
In this study, 20 bacterial isolates were isolated from different organs of the plant. Endophytes were identified using morphological and then molecular characteristics. The antioxidant properties of endophytic extracts were investigated by degradation of free radicals DPPH (DiPhenyl-1-Picryl Hydrazyl free radical).
 
Results
The R4 bacterium with the IC50 value of 2.5 mg / ml had the highest and the SK6 bacterium with an IC50 value of 8.8 mg / ml had the lowest antioxidant activity. The molecular identification of R4 bacteria revealed 98% similarity to the bacterium Rahnella aquatilis. It was also observed by phytochemical analysis that endophytic extracts with phenolic compounds exhibit significant antioxidant activity.
 
Conclusions
Barije has different bacterial endophytes with different antioxidant properties. One of these bacteria is Rahnella aquatilis, which has high antioxidant properties. The presence of phenolic compounds in the extract of this bacterium is probably related to its antioxidant properties.

Keywords

References

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Agricultural Biotechnology Journal
Volume 11, Issue 2
Volume 11, No 2
September 2019
Pages 127-142

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