Bioinformatics and Phylogenetic Analysis of NADH3 and NADH4L mitochondrial genes in Iranian Camelus bactrianus

Document Type : Research Paper

Authors

Abstract

Keeping genetic diversity among native Iranian breeds is very important as a national resource. Studying mitochondrial genome between or within breeds can be a useful indicator of genetic diversity of the population to be studied. The aim of current study was bioinformatic and phylogenetic investigation of NADH3 and NADH4L genes of mitochondrial genome in Camelus bactrianus in Iran. For this purpose, blood samples were collected from 10 Camelus bactrianus in Iran. After extracting DNA, fragment 971 bp of genome mitochondrial of Camelus bactrianus amplified by primers. The amplified fragments were sequenced after purification. Results indicated two different haplotypes based on one single nucleotide polymorphism sequence. The final sequences of each haplotype had a length of approximately 715 bp which included 27/70 % adenine, 13/80 % guanine, 25/60 % cytosine and 32/80 % thymine. Comparison of nucleotide and amino acid sequences of NADH3 and NADH4L genes among Iranian Camelus bactrianus demonstrated that this specie had close genetic distance with domestic Camelus bactrianus. Phylogenetic analysis using Neighbor-Joining method showed that this specie has the lowest similarity with Lama among the Camelidae family.

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Ahmed MM, El-Shazly SA, Sayed SM and Amer SA. (2013). Molecular study of energy related mitochondrial genes in Arabian and bactrian camels.American Journal of Biochemistry and Biotechnology  9:61-70.

Al-Swailem AM, Shehata MM, Abu-Duhier FM, Al-Yamani EJ and Al-Busadah KA et al. (2010). Sequencing, Analysis and Annotation of ExpressedSequence Tags for Camelus dromedaries. PLoS One 5:e10720.
Alinaghizadeh H, Mohammad Abadi MR, Zakizadeh S (2010). Exon 2 of BMP15 gene polymorphism in Jabal Barez Red Goat. Agricultural Biotechnology 2: 69-80.
Ansari-Renani H, Salehi M, Ebadi Z and Moradi S. (2010). Identification of hair follicle characteristics and activity of one and two humped camels. Small Ruminant Research 90(1):64-70.
Beaumont AR and Hoare K. (2003). Biotechnology and genetics in fisheries and aquaculture. Blackwell Science Ltd.
Cui P, Ji R, Ding F, Qi D, Gao H, Meng H, Yu J, Hu S and Zhang H. ( 2007). A complete mitochondrial genome sequence of the wild two-humped camel (Camelus bactrianus ferus): an evolutionary history of camelidae. BMC Genomics 8:241.
Eyre-walker A, Awadalla P. (2001). Does human mtDNA recombine Journal of Molecular Evolution 53: 430-435.
Ghasemi Meymandi M, Mohammadabadi MR, Esmailizadeh AK (2015). Genetic variation of camels in the North of Kerman province using microsatellite markers. Animal Production Research 4: 35-45.
Ji R, Cui P, Ding F, Geng J, Gao H, Zhang H, Hu S and Meng H.(2009). Monophyletic origin of domestic bactrian camel (Camelus bactrianus) and its evolutionary relationship with the extant wild camel (Camelus bactrianus ferus). Animal Genetics 40: 377–382.
Mohammadi A, Nassiry MR, Mosafer J, Mohammadabadi MR, Sulimova GE (2009). Distribution of BoLA-DRB3 allelic frequencies and identification of a new allele in the Iranian cattle breed Sistani (Bos indicus). Russian Journal of Genetics 45: 198-202.
Mousavizadeh A, Mohammad Abadi MR, Torabi A, Nassiry MR, Ghiasi H, AliEsmailizadeh AK (2009). Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian Journal of Biotechnology 7: 51-53.
Nei M and Kumar S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Pirani N, Mohammadhashemi A, Alijani S, Rezazadeh Goli R, Ghanbari S .( 2010). Molecular Analysis of Mazandrani native chicken population based on HVR-I region of Mitochondrial DNA. Journal of Agriculture Biotechnology 1(2): 53- 60.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739.