Mapping of QTLs Affecting Salinity Tolerance in Iranian Rice Population at Germination Stage

Document Type : Research Paper

Authors

1 M.Sc. student of Biotechnology Crop, Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Gonbad, Iran. University Gonbad.

2 Associate professor of plant production department, Gonbad kavus University

3 Horticulture biotechnology Ph.D, Assistant Professor, Department of Plant Production, Faculty of Agricultural and Natural resources Sciences, Gonbad Kavoos University.Iran.

4 Crop physiology Ph.D, Assistant Professor, Department of Plant Production, Faculty of Agricultural and Natural resources Sciences, Gonbad Kavoos University.Iran.

5 Department of Plant Production, Faculty of Agricultural and Natural resources Sciences, Gonbad Kavoos University.Gonbad Kavoos. Iran

Abstract

Objective
This experiment was conducted for developing of linkage map using SSR and ISSR markers in Iranian rice population, mapping of QTLs involved in salinity tolerance, and also evaluation of salinity tolerance in studied genotype.
 
Materials and Methods
In order to determine the salinity tolerance QTLs in a germination stage on an Iranian rice population (caused Ahlami Tarom × Neda cross), a factorial experiment was performed based on a randomized complete block design with three replications under three salinity levels of NaCl (0, 12, 22 dS.m-1).
 
Results
The difference between populations in different levels of salinity was significant for all germination traits. Under normal conditions, 4, 1, 2 and 1 QTLs were detected for radicle weight, plumule weight, germination percentage and germination rate, respectively. In 12 dS.m-1, 1, 1, 1, 2, 1 and 1 QTL for seedling weights, plumule weight, radicle length, coleoptile, germination percentage and germination rate. In 22 dS.m-1, 2, 1, 1 and 1 QTL were located for radicle weight, plant length, germination percentage and germination rate.
 
Discussion
The major effects of QTLs in this study played an important role in salinity tolerance and can be studied in marker assisted selection programs in rice under saline conditions.

Keywords

References

 

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Agricultural Biotechnology Journal
Volume 11, Issue 2
Volume 11, No 2
September 2019
Pages 1-22

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