Reconstruction, analysis and comparison of gene networks topology based on RNA-Seq data involved in reproductive and fertility complex traits.

Document Type : Research Paper

Authors

1 Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran

2 Assistant Professor, Shahid Bahonar University of Kerman, Iran.

Abstract

Objective
 In spite of the importance of fertility in different species, there has been little success dissecting the levels of OMICS. To better understand the molecular basis of fertility, we study transcriptome profiling of different tissues.
 
Materials and methods
 liver, muscle, endometrium and corpus luteum tissues between cows with either good or poor genetic merit for fertility using RNA-Seq data sets. We first compiled a master list of genes related to corpus luteum that change with level of fertility and then reconstructed the network.
 
Results
A few genes were identified in liver, muscle and endometrium between high and low fertile cows but in corpus luteum circumstance was different. 264 genes and 6 key modules were disclosed through clustering for mRNA master list for corpus luteum. All these genes, being involved in at least one of the biological process, namely proteolysis, actin cytoskeleton organization, immune system process, biological adhesion, cell differentiation and lipid metabolic process, have an overexpression pattern (P < 0.01).
Conclusions
Finally, the identification of genes and the construction of their regulatory networks may give new insights into biological procedures. As well as, this study increases our understanding of the contribution of different tissues transcriptome to phenotypic fertility in dairy cattle.

Keywords


پسندیده مجید، خراتی کوپایی حامد، محمدآبادی محمدرضا، اسماعیلی زاده کشکوئیه علی (1395) ارتباط آلل‏های ژن‏های OPN و PPARGC1A با تعداد سلول‏های بدنی شیر در گاوهای هلشتاین ایران. مجله ژنتیک نوین 11(3)، 365-357.
توحیدی نژاد فاطمه، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، نجمی نوری عذرا (1393) مقایسه سطوح مختلف بیان ژنRheb  در بافت های مختلف بز کرکی راینی. مجله بیوتکنولوژی کشاورزی 6(4)، 50-35.
جعفری دره در امیر حسین، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، ریاحی مدوار علی (1395) بررسی بیان ژن CIB4  در بافت­های مختلف گوسفند کرمانی با استفاده از Real Time qPCR. مجله پژوهش در نشخوارکنندگان 4(4)، 132-119.
خراتی کوپایی حامد، محمدآبادی محمدرضا، انصاری مهیاری سعید، اسماعیلی زاده کشکوئیه علی، ترنگ علیرضا، نیکبختی مهدی (1390) بررسی تنوع ژنتیکی جایگاه ژن DGAT1 و میزان ارتباط آن با تولید شیر در جمعیت گاو های هلشتاین ایران.مجله پژوهش­های علوم دامی ایران 3(2)، 192-185.  
خراتی کوپایی حامد، محمدآبادی محمدرضا، ترنگ علیرضا، خراتی کوپایی محمود، اسماعیلی زاده کشکوئیه علی (1391) بررسی ارتباط چند شکلی آللی ژن DGAT با بیماری ورم پستان در جمعیت گاوهای هلشتاین ایران. مجله ژنتیک نوین 7(1)، 104-101.
محمدآبادی محمدرضا، محمدی اکرم (1389) بررسی ژنوتیپ های بتالاکتوگلوبولین در گاوهای بومی و هلشتاین استان کرمان. مجله تولیدات دامی 12(2)، 67-61.
 
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