Phylogenetic Analysis of Cytochrome B of Mitochondrial Genome in some Iranian Goat Breeds

Document Type : Research Paper

Authors

1 Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran

2 MSc Graduated, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj

3 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj

4 Assistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran.

5 Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran.

Abstract

Objective
Identifying the genetic diversity of native goat breeds of Iran can play an important role in the management and conservation of these breeds. The purpose of the current study was genetic and phylogenetic investigation of mitochondrial genome cytochrome b region in Lori, Pakistani, Zaboli and Adeni goats.
 
Materials and Methods
Blood samples were collected from 16 goats of each breed 4 blood samples. After extracting DNA, fragment 684 bp of cytochrome b region genome mitochondrial amplified by primers were designed and fragment amplified after purification was sequenced. Using the DNAsp software was determined in six different haplotypes.
 
Results
 The result of comparative analysis between goat breeds indicates that nucleotide diversity Pakistani and Zaboli breeds 0.35461 and 0.11199 respectively. Tajima D was obtaining 1.06945 that was significant. This confirmed the evolution by natural selection. Results from the analysis of molecular variance indicated that genetic variation was 80% of within and 20% was between populations that indicates high gene flow, migration among the studied populations or little sample size.
 
Conclusions
Based on a phylogenetic tree analysis, Lori and adni goat form a group with Capra hircus and Pakistani and Zabli goats form a separate group. This indicates the goat breeds have a common origin area.

Keywords

References

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Agricultural Biotechnology Journal
Volume 11, Issue 4
Volume 11, No 4
December 2019
Pages 19-34

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