Investigation of heat shock protein 70 gene polymorphism in Khuzestan native chickens

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

2 Assistant Professor, Department of Venomous Animals and Anti-venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran.

3 PhD Student of Animal Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran,

Abstract

 
Objective
Heat shock protein 70 (Hsp70) are a family of molecular chaperones, which promote protein folding and participate in many cellular functions. The purpose of the study was to identify the polymorphisms of heat shock protein 70 gene of Khuzestan native chickens using SSCP technique.
 
Materials and methods
For this purpose, blood samples were collected from 130 native chicken from Shosh, Ramhormoz, Andimeshk cities and Jihad native chicken breeding station (Bavi city). After DNA extraction, the target area (892 base pair) was amplified using polymerase chain reaction (PCR) method and sequenced. 
 
 
Results
PCR-SSCP analysis of these regions indicated the presence of polymorphisms in the beginning of the coding region and the sequencing of the PCR products confirmed and identified two polymorphic sites in this region: a transition A → G in position +259 (A259G) and a transversion C→G in position +277 (C277G). The results showed that Hsp70 gene is polymorphic in the studied area. Moreover, using the chi-square (X2) showed that the locus allele frequencies is not in Hardy-Weinberg equilibrium. Furthermore, the observed heterozygosity and the effective number of alleles demonstrated high diversity in the population. Moreover, Haplotype H3 has the highest frequency in the population. It occurs A259G mutants in this haplotype. 
 
Conclusions
Thus, these valuable gene pools should be conserved to be used in the future if needed. The selection at Jihad native chicken breeding station (Bavi city) has led to increased inbreeding and, as a result, the abundance of haplotype H3 in the population.

Keywords

References

 
توحیدی نژاد فاطمه، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، نجمی نوری عذرا (1393) مقایسه سطوح مختلف بیان ژنRheb  در بافت های مختلف بز کرکی راینی. مجله بیوتکنولوژی کشاورزی 6(4)، 50-35. 
جعفری دره در امیر حسین، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، ریاحی مدوار علی (1395) بررسی بیان ژن CIB4  در بافت­های مختلف گوسفند کرمانی با استفاده از Real Time qPCR. مجله پژوهش در نشخوارکنندگان 4(4)، 132-119.
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Agricultural Biotechnology Journal
Volume 12, Issue 1
April 2020
Pages 81-100

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