Evaluation of hormone content in transgenic rose (Rosa hybrida L.) with etr1-1 gene treated by ethylene and gibberellin

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Plant Science, Faculty of Biological Science, Kharazmi University, P.O. Box: 1571914911, Tehran, Iran.

2 Associate Professor, Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code: 1571914911, Tehran, Iran.

3 Assistant Professor, Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Postal Code: 33535111, Tehran, Iran.

4 Professor, Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal Code: 1571914911, Tehran, Iran; and Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Objective
Roses are one of the most important cut flowers in the world and therefore many studies have focused on keeping post-harvest quality and longevity. Ethylene is one of the major factors that limits post-harvest longevity. Therefore, genetic manipulation has been considered to reduce the adverse effects of this hormone and the present research investigates transgenic roses containing the mutant etr1-1 gene. In addition, according to antagonist effects, the change of gibberellin concentration to ethylene in favor of higher gibberellin concentration in the present research has been studied.
 
 
 
Materials and methods
The content of phytohormones in long-lasting line (transgenic) and control (non-transgenic) after application of ethylene (0 and 1 µL L-1) and gibberellin (80 mg L-1) treatments were investigated in bud and half-open stages. Application of ethylene treatment was performed by injecting pure gas into polyethylene bags by syringe. For the application of gibberellin treatment, cut flowers were placed in gibberellin solution at the desired concentration. The treatments were applied for 24 hours and samples were taken from the outermost ring of petals. Experiments were conducted as factorial in a completely randomized design with four replications in order to measure phytohormones.
 
Results
The results showed a decrease in the amount of gibberellin, benzyl adenine and indole acetic acid hormones and an increase in the amount of abscisic acid and ethylene hormones in bud and half-open stages in long-lasting line were significant compared to the control. In addition, the highest content of gibberellin, indole acetic acid and benzyl adenine hormones and the lowest content of ethylene and abscisic acid belongs to the GA3-treated long-lasting line.
 
Conclusion
Therefore, etr1-1 gene appears to be the right candidate for delaying ethylene-dependent aging in sensitive flowers and with GA3 treatment could be considerably improved longevity by reduction of oxidative damage. 

Keywords

References

خاتمی فائزه؛ نجفی فرزانه؛ یاری فتانه؛خاوری­نژاد رمضانعلی (۱۳۹۷) ژن etr1-1 کاندید برتر جهت دست­ورزی ژنتیکی مسیر پیام رسانی علامت هورمون اتیلن در گل سرخ. مجله پژوهش­های گیاهی جلد ۳۱، شماره ۱.
خاتمی فائزه؛ نجفی فرزانه؛ یاری فتانه؛خاوری­نژاد رمضانعلی (۱۳۹۵) بررسی تغییرات سوماکلونال در لاین­های تراریخته گل رز. نهمین کنگره علوم باغبانی ایران، اهواز.
یاری فتانه (۱۳۹۵) فرآیند رز ماندگار غیرحساس نسبت به اتیلن حاوی ژن موتانت ETR1-1. ثبت اختراع ایرانی: ۹۵۷۶۰.
 
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Agricultural Biotechnology Journal
Volume 12, Issue 2
September 2020
Pages 1-20

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