Marker association and genetic variation of agronomic traits in safflower )Carthamus tinctorius L.( using AFLP marker

Document Type : Research Paper

Authors

1 . M. Sc. Graduate, College of Agriculture, Shahid Bahonar University of Kerman

2 Assistant Professor, Research & Technology Institute of Plant Production, Shahid Bahonar University of Kerman, Iran.

3 Associate Professor, College of Agriculture and Research & Technology Institute of Plant Production, Shahid Bahonar University of Kerman, Iran

Abstract

Objective
This experiment was conducted in order to assess genetic variation, determine of the best genetic structure and association analysis of safflower to identify markers associated with different agronomic traits.
 
          Materials and methods
In this study, 17 genotypes of safflower were planted as the randomised complete block design at research farm of Shahid Bahonar University of Kerman in 2016. Traits including seed yield, plant height, number of capitulum per plant, number of seed per capitulum, 1000- seed weight, capitulum diameter, day to 50% flowering and day to maturity were measured. The AFLP Technique was performed by the eight EcoR1/Mse1 primer combinations.
          Results
In total, 147 polymorphic bands were generated with average 81.58 polymorphic percentage. Cluster analysis using UPGMA and Jacard as similarity index discriminated safflower genotypes into two groups. Based on GLM and MLM model, 45 and 39 markers had significant association with the studied traits, respectively. M14/E6-10, M14/E11-16, M14/E11-13, M3/E10-14 and M4/E36-12 markers with seed yield, M3/E10-12, M3/E36-29, M59/E36-21 and M14/E11-10 with capitulum number per plant, M4/E36-8, M59/E36-21, M3/E10-9, M14/E11-14 and M14/E11-13 with number of seed per capitulum, M4/E36-19, M4/E36-12, M14/E11-1 and M4/E10-1 with 1000- seed weight, M4/E10-2, M59/E36-21, M3/E36-30, M4/E10-11 and M4/E36-24 with plant height, M3/E36-24, M3/E36-6, M3/E10-20 and M4/E36-18 with capitulum diameter, M14/E11-10, M3/E36-30 and M59/E36-21 with day to 50% flowering, M14/E11-10 and M59/E36-22 markers with day to maturity in both models.
 
         Conclusions
Detected AFLP markers with strong effects in this study could be desirable                                                                                                                                              candidates for the marker-assisted selection in breeding programs and conversion into other specific markers.

Keywords

References

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Agricultural Biotechnology Journal
Volume 12, Issue 2
September 2020
Pages 43-62

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