Study of genetic diversity of some genotypes of iranian wild mint (Mentha longifolia L.) using ISSR marker and its correlation with dry yield and essential oil content

Document Type : Research Paper

Authors

1 Department of horticulture, faculty of agriculture, ferdowsi university of mashhad, mashhad, Iran.

2 Assistant Professor, Department of Horticulture Sciences, Faculty of Agriculture,

3 Department of horticulture, faculty of agriculture, ferdowsi university of mashhad

4 Associated Professor, Department of Plant Science and Biotechnology, Faculty of Agriculture,

5 Associated Professor, Department of Horticulture Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad,

Abstract

Objective
This study aimed to investigate the genetic diversity of different genotypes of this valuable medicinal plant under the same culture condition which can be used as an introduction to domestication, germplasm conservation, and possible cross in the future.
 
Materials and Methods
In this study, after preparing 20 different genotypes from all over Iran, they were cultivated in complete randomized blocks design with three replications under the same condition. After extraction of DNA, the survey of genetic diversity using 12 ISSR markers from 15 markers was conducted by polymerase chain reaction (PCR). The essential oil for each genotype was extracted by water distillation. The dry yield based on gr/m2 and essential oil content (w/w, based on dry weight) were measured. Furthermore, the correlation of molecular markers with dry yield and essential oil content from each genotype was determined using stepwise regression. 
 
Results
The mean percentage of polymorphism determined in all the genotypes was 91.97. The number of polymorphic bands for each primer varied from 5 to 9 and a total of 89 replicate bands were scored, of which 82 bands showed polymorphism. The average content of primer information polymorphism (PIC) was estimated to be 0.31 and the IS1 primer showed the highest PIC (0.46). The Ni and Shannon indices for IS1 primers were 0.43 and 0.61, respectively. Cluster analysis using the Jaccard similarity coefficient and UPGMA algorithm divided the studied genotypes into four groups. The highest genetic distance was observed between Khuzestan and Qazvin genotypes with a coefficient of 0.39 and the lowest between Kerman-2 and Kerman-4 genotypes with a similarity coefficient of 0.77. Stepwise regression showed that the IS10 primer has a coefficient of 0.70 with the essential oil percentage and dry matter yield.
 
Conclusion
The results of this study showed that ISSR markers can be effectively used to study the genetic diversity of wild mint genotypes and will provide the possibility of breeding. So that, IS1 and IS10 primers were introduced as the best markers. Also, Khuzestan and Qazvin genotypes had the highest genetic distance.

Keywords

References

خورشیدی جلال؛ شکرپور مجید؛ ناظری وحیده (1397) ارزیابی تنوع ژنتیکی جمعیت‌‌‌های مختلف آویشن دنایی (Thymus daenensis Celak) با استفاده از نشانگر ISSR. مجله بیوتکنولوژی کشاورزی 10 ، 74-59.
زین‌‌‌الدینی آرش؛ فرشادفر محسن؛ صفری هوشمند؛ مرادی فرزاد؛ شیروانی هومن (1392) مطالعه روابط ژنتیکی برخی از گونه‌‌‌های مختلف نعناع با استفاده از نشانگر ISSR. مجله زیست‌‌‌فناوری گیاهان زراعی 5، 21-11.
سقلی عزیزه؛ فرخاری محمد؛ صلواتی افشین؛ عالمی سعید خلیل؛ ابدالی مشهدی علیرضا (1395) بررسی تنوع ژنتیکی اکوتیپ‌‌‌های گیاه دارویی خارمریم (Silybum marianum L.) با استفاده از نشانگر ISSR. مجله بیوتکنولوژی کشاورزی 8 (3)، 64-51.
رحیم‌‌‌ملک مهدی؛ خرمی مجتبی؛ غریبی شیما؛ زینلی بادی حسین؛ طالبی مجید (1391) بررسی تنوع ژنتیکی برخی از نمونه‌‌‌های جمعیتی Mentha Spicata L. و روابط آن با دو گونه M. piperita L. و M. longifolia با استفاده از نشانگرهای ISSR و مورفولوژیک. مجله علوم و فنون باغبانی ایران 13، 126-115.
حسنپور ریحانی کبری؛ سفالیان امید؛ زارع ناصر؛ اصغری علی؛ اسماعیل‌‌‌پور بهروز (1396) بررسی تنوع ژنتیکی و مورفولوژیکی اکوتیپ‌‌‌های پونه Mentha longifolia بومی ایران. مجله ژنتیک نوین 4، 625-617.
حسین‌‌‌جعفری سمیرا؛ سعادت‌‌‌فر امیر؛ محکمی افسانه؛ کریمیان علی‌‌‌اکبر (1398) بررسی تنوع ژنتیکی و فیتوشیمیایی زیره سبز کشت شده در مراتع مختلف استان یزد. مجله بیوتکنولوژی کشاورزی 11 ، 66-51.
 
References
Aga E, Bekele E, Bryngelsson T (2005) Inter-simple sequence repeat (ISSR) variation in forest coffee trees (Coffea arabica L.) populations from Ethiopia. Jordan J Biol Sci 124, 213-221.
Al-Rawashdeh IM (2011) Molecular taxonomy among Mentha spicata, Mentha longifolia and Ziziphora tenuior populations using the RAPD technique. Jordan J Biol Sci 147, 1-8.
Commission BP (1993) British Pharmacopoeia 1993.
Doyle JJ, Doyle JL (1990) Isolation ofplant DNA from fresh tissue. Focus 12, 39-40.
Figueiredo AC, Barroso JG, Pedro LG et al. (2008) Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Frag J 23, 213-226.
Hassanpor Reihani K, Sofalian O, Zare N et al. (2018) Evaluation of genetic, morpho-physiological diversity in Iranian Mentha longifolia ecotypes. Mod Genet J 12, 617-625 (In Persian).
Heydari A, Hadian J, Esmaeili H et al. (2019) Introduction of Thymus daenensis into cultivation: Analysis of agro-morphological, phytochemical and genetic diversity of cultivated clones. Ind Crops Prod 131, 14-24.
Hossein Jafari S, Saadatfar A, Mohkami A et al. (2020) Investigating genetic and phytochemical diversity of cumin ecotypes cultivated in different rangelands of yazd province. Agric Biotechnol J 11, 51-66 (In persian).
Khorshidi J, Shokrpour M, Nazeri V (2018) Assessment of genetic diversity in different populations of Thymus daenensis Celak. using ISSR marker. J Agric Biotechnol 10, 59-74 (In Persian).
Meimberg H, Abele T, Bräuchler C et al. (2006) Molecular evidence for adaptive radiation of Micromeria Benth.(Lamiaceae) on the Canary Islands as inferred from chloroplast and nuclear DNA sequences and ISSR fingerprint data. Mol Phylogenet Evol 41, 566-578.
Mirzaie‐Nodoushan H, Rezaie MB, Jaimand K (2001) Path analysis of the essential oil‐related characters in Mentha spp. Flavour Frag J 16, 340-343.
Moshrefi-Araghi A, Nemati H, Azizi M et al. (2019) Assessment of phytochemical and agro-morphological variability among different wild accessions of Mentha longifolia L. cultivated in field condition. Ind Crops Prod 140, 111698.
Moshrefi-Araghi A, Nemati SH, Shoor M et al. (2018) Influence of Water Stress on Agro-Morphological Traits and Essential Oil Content Among Iranian Genotypes of Mentha longifolia. Proc Natl Acad Sci India Sect B Biol Sci, 1-12.
Nazem V, Sabzalian MR, Saeidi G et al. (2019) Essential oil yield and composition and secondary metabolites in self-and open-pollinated populations of mint (Mentha spp.). Ind Crops Prod 130, 332-340.
Nei M (1972) Genetic distance between populations. Am Nat 106, 283-292.
Nybom H, Bartish IV (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspect Plant Ecol 3, 93-114.
Okut N, Yagmur M, Selcuk N et al. (2017) Chemical composition of essential oil of Mentha longifolia L. Subsp. Longifolia growing wild. Pak J Bot 49, 525-529.
Petrova G, Petrov S, Delcheva M et al. (2017) Genetic diversity and conservation of Bulgarian endemic Verbascum tzar-borisii (Scrophulariaceae). In: Ann Bot Fenn. BioOne. pp. 307-316.
Powell W, Morgante M, Andre C et al. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol breeding 2, 225-238.
Rahimmalek M (2011) Study of genetic relationships of some mint species using R-ISSR markers. Agric Biotechnol 10, (In persian).
Rahimmalek M, Khorami M, Gharibi S et al. (2012) Assessment of genetic diversity of some Mentha spicata L. accessions and their relationships with M.piperita L. and M. longifolia L. using inter simple sequence repeat (ISSR) ans morphological markers. Int J Hort Sci Tech 13, 115-126 (In Persian).
Reddy AR, Chaitanya KV, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161, 1189-1202.
Rodrigues L, van den Berg C, Póvoa O et al. (2013) Low genetic diversity and significant structuring in the endangered Mentha cervina populations and its implications for conservation. Biochem Syst Ecol 50, 51-61.
Rostami-Ahmadvandi H, Cheghamirza K, Kahrizi D et al. (2013) Comparison of morpho-agronomic traits versus RAPD and ISSR markers in order to evaluate genetic diversity among Cuminum cyminum L. accessions. Aust J Crop Sci 7, 361.
Santhosh W, Shobha D, Melwyn G (2009) Assessment of genetic diversity in cashew germplasm using RAPD and ISSR markers. Sci Hortic 120, 411-417.
Selseleh M, Hadian J, Ebrahimi SN et al. (2019) Metabolic diversity and genetic association between wild populations of Verbascum songaricum (Scrophulariaceae). Ind Crops Prod 137, 112-125.
Shaw RG, Etterson JR (2012) Rapid climate change and the rate of adaptation: insight from experimental quantitative genetics. New Phytol 195, 752-765.
Shokrpour M, Mohammadi SG, Moghadam M et al. (2008) Analysis of morphologic association, phytochemical and AFLP markers in milk thistle (Silybum marianum L.). Iranian J Med and Aroma Plants Res 24, 278-292 (In Persian).
Smolik M, Jadczak D, Rzepka-Plevneš D et al. (2007) Morphological and genetic variability of chosen Mentha species. Herba Polonica 53, 90-97.
Tucker AO, Naczi RF (2007) Mentha: an overview of its classification and relationships. Mint: the genus Mentha CRC Press, Boca Raton, 1-39.
Volis S, Yakubov B, Shulgina I et al. (2001) Tests for adaptive RAPD variation in population genetic structure of wild barley, Hordeum spontaneum Koch. Biol J Linn Soc 74, 289-303.
Zhao D, Xu YW, Yang GL et al. (2013) Variation of essential oil of Mentha haplocalyx Briq. and Mentha spicata L. from China. In: Ind Crops Prod. pp. 251-260.
Zinodini A, Farshad Far M, Safari H et al. (2014) Study of genetic relationships of some mint species using ISSR markers. Crop Biotech 3, 11-21 (In Persian).
Agricultural Biotechnology Journal
Volume 12, Issue 3
November 2020
Pages 117-140

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