Construction of a polyepitope vaccine against Newcastle disease by immunoinformatics design of immunogenic proteins

Document Type : Research Paper

Authors

1 Zahra Roudbari, Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

2 Sari University of Agricultural Sciences and Natural Resources, Sari, Iran Department of Animal Science and fishery

3 Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran

Abstract

Objective
Application of molecular biology techniques to the production of new vaccines against different strains of the Newcastle disease virus (NDV) has been the subject of recent research reports. Development of improved techniques for genome sequencing has led to the recognition of protective mechanisms and the identification of possible candidate antigens. The present research aimed to design a recombinant chimeric immunogen against binding agents in Newcastle virus in birds.
 
Materials and methods
Therefore, we conducted this investigation to make a recombinant vaccine in silico in order to control and eliminate this disease using anti-leptospirosis epitopes, F, and HN antigens. To bind these epitopes, KK, as AAY linkers, were selected to bind the structure. This structure contained 309 amino acids. The important biological factors of this recombinant vaccine, such as physicochemical properties, different structures, stability, intrinsic protein disorder, solubility, and allergenicity of this vaccine structure were evaluated using immune system analysis.
 
Results
Various analyses confirmed the stability of this structure, and the epitopes predicted in the chimeric vaccine showed a high potential for inducing the immune response of B cell and T cell. Thus, immune system analysis revealed that the modeled multi-epitope vaccine could properly stimulate T and B cell immune responses and could potentially be utilized for prophylactic or control applications.
 
Conclusion
The results of this study could be employed to control and eliminate leptospirosis in the future after confirming its effectiveness by experimental immunological assays

Keywords

References

 
 
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Agricultural Biotechnology Journal
Volume 13, Issue 2
July 2021
Pages 171-188

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