Evaluation of genetic relationships of Aegilops species with targeted region amplified polymorphism (TRAP) markers

Document Type : Research Paper

Authors

1 M.Sc. Student of Genetics & Plant Breeding, Imam Khomeini International University, Iran.

2 Corresponding author. Associate Professor, Department of Genetics & Plant Breeding, Imam Khomeini International University, Qazvin, Iran.

3 Research associate, Department of Biotechnology, Research Institute of Forest and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Objective
The aim of this study was to determine the relationships and genetic diversity between and within wild species of Aegilops using EST-derived TRAP molecular markers.
 
Materials and Methods
In this study, genetic diversity and relationships among eight wild species of Aegilops collected from 14 provinces of Iran were evaluated using 24 TRAP primer combinations.
 
Results
The mean of PIC for studied primers was 0.95 and the lowest and highest values ​​of this index were related to TRAP20 (0.92) and TRAP10 (0.97), respectively. Also, the mean of MI was 10.77 and the lowest and highest values ​​were related to TRAP3 (6.95) and TRAP17 (17.34), respectively. The molecular analysis of variance showed 60% variance between species and 40% variance within species. Estimated genetic parameters showed that the highest levels of Na (1.07), Ne (1.26), I (0.23) and uHe (0.16) belonged to Ae. truncialis and the maximum Nei gene diversity (0.14) was related to Ae truncialis, Ae. umbelulata and Ae. neglecta. Minimum values ​​of Nei gene diversity (0.09), Shannon index (0.14), No. of effective alleles (1.16) and No. of observed alleles (0.79) was observed for Ae. crassa. The least genetic similarity was observed between Ae. crassa with Ae. cylandrica and Ae. umbelulata and the most similarity between Ae. triuncialis and Ae. umbelulata. Being in the same group of Ae. umbelulata and Ae. truncialis with cluster analysis and PCOA confirmed the high genetic similarity of these species.
 
Conclusions
The results showed high genetic diversity among and within species. Molecular analysis of variance revealed higher genetic diversity between species, indicating low gene flow and Fst, as well as a high genetic differentiation between species.

Keywords

References

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Agricultural Biotechnology Journal
Volume 14, Issue 2
May 2022
Pages 67-84

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