Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar)

Document Type : Research Paper

Authors

1 MSC Student, Department of Plant Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous University

2 Assistant Professor, Department of Plant Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous University

3 Faculty member of Agricultural Biotechnology Research Institute (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj

Abstract

Objective
Nanotechnology, as a promising method for addressing sustainable agricultural issues, can increase propagation efficiency in palm tissue culture. This study aimed to prepare carbon nanoparticles and use the resulting nanocomposites to evaluate their efficiency in improving and increasing date callus formation.
Materials and methods
Three separate experiments were performed to propagate calluses consisting of meristematic microsamples of dates. In the first experiment, calli prepared in MS culture medium were transferred to four culture media with different hormonal treatments from NAA and 2iP, and in the second experiment, calli composed of meristematic date microsamples in MS culture medium were transferred to four culture media with separate treatments from NAA and BAP. Different hormones were transferred from NAA and BAP. In the third experiment, after determining the best hormonal treatments from the first and second experiments, carbon nanoparticles were synthesized from graphite, and calli composed of meristematic date microsomal samples were recreated in superior culture media with different concentrations of nanoparticles (0, 10, 20, 30, 40, 50 mg/L).
Results
Based on the results of the first experiment, treatments of 10 mg/L NAA + 30 mg/L 2ip and 0.1 mg/L NAA + 0.05 mg/L 2ip were selected as the best callus propagation treatments. In the second experiment, it was found that there was no statistically significant difference between the applied treatments of 10 mg/L NAA + 30 mg/L BAP and 10 mg/L NAA + 10 mg/L BAP with other treatments with different concentrations of BAP. The results of the third experiment showed that the use of 10 mg/L NAA + 30 mg/L BAP + 30 mg/L CNP can produce the most calluses.
Conclusions
Due to the positive effect of carbon nanoparticles on increasing the weight of commodities, treatment of 10 mg/L NAA + 30 mg/L BAP + 30 mg/L CNP becomes the most suitable option for calorific cultivar propagation.

Keywords


حبشی علی‌اکبر، موسوی امیر، کاویانی مینا، خوشکام صغری و رستمی علی‌مردان (1387) ریزازدیادی خرما از طریق جنین‌زایی رویشی. علوم و فنون کشاورزی و منابع طبیعی 12، 7-1.
References
Abahmane L (2017) Cultivar-dependent direct organogenesis of date palm from shoot tip explants. Methods Mol Biol 1637, 3-15.
 Al-Khayri JM, Jain S, Johnson D (2017) Date palm biotechnology protocols (1st edn), Volume I. Tissue culture applications. Springer, New York 3–15.
Al-Khayri JM (2010) Somatic embryogenesis of date palm (Phoenix dactylifera L.) improved by coconut water. Biotechnol J 9, 477-484.
Al-Khayri JM (2011) Basal salt requirements differ according to culture stage and cultivar in date palm somatic embryogenesis. Am J Biochem Biotechnol 7, 32-42.
Alvarez SP, Tapia MA, Vega ME et al. (2019) Nanotechnology and Plant Tissue Culture. In Plant Nanobionics pp.333-370.
Asemota O, Eke CR, Odewale JO (2007) Date palm (Phoenix dactylifera L.) in vitro morphogenesis in response to growth regulators, sucrose and nitrogen. Afr J Biotech 6, 2353-2357.
Chhipa H  (2017a)  Nanofertilizers and nanopesticides for agriculture. Environ 15, 15–22
Chhipa H (2017b)  Nanopesticide: current status and future possibilities. Agri Res Technol J 5, 1-4
Chhipa H, Joshi P (2016) Nanofertilisers, nanopesticides and nanosensors in agriculture. In: Nanoscience in Food and Agriculture 1. Springer 247–282.
Duhan JS, Kumar R, Kumar N et al. (2017) Nanotechnology: the new perspective in precision agriculture. Biotech Rep 15, 11–23.
Fazal H, Abbasi BH, Ahmad N, Ali M (2016) Elicitation of Medicinally Important Antioxidant Secondary Metabolites with Silver and Gold Nanoparticles in Callus Cultures of Prunella vulgaris L. Appl Biochem Biotech 180, 1076–1092.
Flores D, Chacón R, Alvarado L et al. (2014) Effect of using two different types of carbon nanotubes for blackberry (Rubus adenotrichos) in vitro plant rooting, growth and histology. Am J Plant Sci 5, 3510.
Fraceto LF, Grillo R, Medeiros GA et al. (2016) Nanotechnology in agriculture: which innovation potential does it have? Front. Environ Sci 4- 20.
Ghorbanpour M, Hadian J (2015) Multi-walled carbon nanotubes stimulate callus induction, secondary metabolites biosynthesis and antioxidant capacity in medicinal plant Satureja khuzestanica grown in vitro. Carbon 94, 749-759.
Goleyjani MR, Motallebi M, Zamani MR, Rezanejad H (2012) Optimization of regeneration and transformation of canola Hyola 308 and RGS003 lines. Iran J Plant Biol 4, 47-60.
Habashi A, Mousavi A, Kaviani M, Khoshkam S, Rostami A (2008) Micropropagation of Date Palm via Somatic Embryogenesis. J Child Psychol Psychiatry 12, 1-7
Heidarpour F, Mohammadabadi MR, Zaidul ISM et al. (2011) Use of prebiotics in oral delivery of bioactive compounds: a nanotechnology perspective. Pharmazie 66 (5), 319-324.
Helaly MN, El-Metwally MA, El-Hoseiny H et al. (2014) Effect of nanoparticles on biological contamination of in vitro cultures and organogenic regeneration of banana. Aust J Crop Sci 8, 612-624.
Hummers JR, William S, Richard E (1958) Offeman. "Preparation of graphitic oxide. J Am Chem Soc 80.6: 1339-1339.
Javed R, Mohamed A, Yücesan B et al. (2017) CuO nanoparticles significantly influence in vitro culture, steviol glycosides, and antioxidant activities of Stevia rebaudiana Bertoni. Plant Cell Tiss Organ Cul 131, 611-620.
Kavianifar S, Ghodrati K, Naghdi H,  Etminan A (2018) Effects of Nano Elicitors on Callus Induction and Mucilage Production in Tissue Culture of Linum usitatissimum L. J Med Plant Res 17, 45-54.
Khadri H, Alzohairy M, Janardhan A et al. (2013) Green synthesis of silver nanoparticles with high fungicidal activity from olive seed extract. Nanopartic 2(3):241-246.
Khierallah HS, Bader SM, Al-Khafaji MA (2017) NAA-induced direct organogenesis from female immature inflorescence explants of date palm. Date Palm Biotechnology Protocols Volume 1. 1637, 17-25.
Khodakovskaya MV, De Silva K, Biris AS et al. (2012) Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano 6, 2128–2135.
Khodakovskaya MV, Kim BS, Kim JN et al. (2013) Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Small 14: 115-23.
Kim DH, Gopal J, Sivanesan I (2017) Nanomaterials in plant tissue culture: the disclosed and undisclosed. RSC advances 7, 36492-36505.
Malik WA,  Mahmood I, Razzaq A et al. (2021) Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivum L.). GM Crops Food 12, 1–22.
Mazri MA, Belkoura I, Meziani R et al. (2017) Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda. 3 Biotech 7, 58.
Mohammadabadi MR, El-Tamimy M, Gianello R, Mozafari MR (2009) Supramolecular assemblies of zwitterionic nanoliposome-polynucleotide complexes as gene transfer vectors: Nanolipoplex formulation and in vitro characterization. J Liposome Res 19 (2), 105-115.
Mohammadabadi MR, Mozafari MR (2018) Enhanced efficacy and bioavailability of thymoquinone using nanoliposomal dosage form. J Drug Deliv Sci Technol 47 (1), 445–453.
Mohammadabadi MR, Mozafari MR (2019) Development of nanoliposome-encapsulated thymoquinone: evaluation of loading efficiency and particle characterization. J Biopharm 11 (4), 39-46
Morsy MK, Khalaf HH, Sharoba AM et al. (2014) Incorporation of essential oils and nanoparticles in pullulan films to control foodborne pathogens on meat and poultry products. J Food Sci 79, M675–M684.
Mortazavi SM, Mohammadabadi MR, Mozafari MR (2005) Applications and in vivo behaviour of lipid vesicles. Nanoliposomes From Fundamentals to Recent Developments. Trafford Publishing. England. 67-76
Pandey K, Lahiani MH, Hicks VK et al. (2018) Effects of carbon-based nanomaterials on seed germination, biomass accumulation and salt stress response of bioenergy crops. PloS one 13, 1-17.
Rad MR, Zarghami R, Hassani H, Zakizadeh H (2015)  Comparison of vegetative buds formation in two date palm cultivars, Medjool and Mazafati through direct organogenesis. Int J Farm Alli Sci 4, 549-553.
Rohim FM, El-Wakeel H, El-Hamid A et al. (2020) Impact of Nanoparticles of In Vitro Propagation of Date Palm cv. Barhee by Immature Inflorescences. Arab Universities J Agric Sci 28, 1187-1202.
Salaün C, Lepiniec L, Dubreucq B (2021) Genetic and Molecular Control of Somatic Embryogenesis. Plants 10, 1467.
Sané D, Aberlenc-Bertossi F, Diatta LI et al. (2012) Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars. Sci World J  1-8.
Sarmast MK, Salehi H (2016) Silver Nanoparticles: An Influential Element in Plant Nanobiotechnology. Mol Biotech 58, 441-449.
Saxena R, Kumar M, Tomar RS (2020) Implementation of Nanotechnology in Agriculture System: A Current Perspective. Nanobiotech pp.211-227.
 Shang Y,   Hasan Md,   Ahammed  G et al. (2019) Applications of Nanotechnology in Plant Growth and Crop Protection: A Review. Molecules 24, 2558.
Solanki P, Bhargava A, Chhipa H et al. (2015) Nano-fertilizers and their smart delivery system. In: Nanotechnologies in Food and Agriculture. Springer 81–101.
Taha RA, Hassan MM, Ebrahim EA et al. (2016) Carbon nanotubes impact on date palm in vitro cultures. Plant Cell Tiss Organ Cult 127, 525–534.
Wu T,  Li J, Zhang J et al. (2018) Graphene oxide inhibits the lethal browning of Cymbidium sinense by reducing activities of enzymes. J Plant Biotech Microbiol 1, 20-29.
Zarrabi A, Alipoor Amro Abadi M, Khorasani S et al. (2020) Nanoliposomes and Tocosomes as Multifunctional Nanocarriers for the Encapsulation of Nutraceutical and Dietary Molecules. Molecules 25 (3), e638.
Zaytseva O, Neumann, G (2016) Phytotoxicity of carbon nanotubes is associated with disturbances of zinc homeostasis. Eur Chem Bull 5, 238–244.
Zhang M, Gao B, Chen J, Li Y (2015) Effects of graphene on seed germination and seedling growth. J Nanopart Res 17, 78.