Evaluation of expression pattern of several pathogenesis related genes and PAL in sour orange plant following treatment with SA and JA in response to citrus blast bacterium Pseudomonas viridiflava

Document Type : Research Paper

Authors

1 Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University. Sari/ Iran

2 Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University

3 Department of Plant Protection, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran

4 Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

5 Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Abstract

Objective
Plant diseases are one of the major limitations in agricultural production. Citrus blast disease is one of the most common diseases in citrus producing regions of the world, with the exception of tropical regions. Plants respond to bacterial pathogens by activating genes encoding defense proteins. One of the defense mechanisms of plants against pathogens is the accumulation of Pathogenesis related proteins associated. The aim of this study was to evaluate the expression of several defense genes against Pseudomonas viridiflava after treatment of seedlings with Salicylic acid and Jasmonic acid.
Materials and methods
The expression levels of PR1, PR2, PR3, PR4, PR5, POX, LOX pathogenesis related and PAL genes in the early stages of citrus blast disease in Citrus aurantium were assessed using RT-PCR. In this study, UBI gene was used as a housekeeping gene. The total RNA of the samples was extracted and after cDNA synthesis, the expression level changes of the declared genes were calculated and measured using the formula 2 - (ΔΔCT).
Results
Increased expression of PR1, PR2, PR3, PR4, PR5, POX, LOX Pathogenesis related and PAL genes in the early stages of citrus blast disease in sour orange mostly occurred at 24 and 48 hours after challenging with the citrus blast causal agent P. viridiflava.
Conclusions
The results of this study was showed that salicylic acid treatment had a greater effect on the expression of PR1, PR2, PAL, POX and PR5 genes than jasmonic acid and jasmonic acid treatment was showed a greater effect on the induction of PR4, PR3 and LOX genes than salicylic acid. Furthermore, the expression of PR1, PR2, LOX, PR4, PAL and PR5 genes peaked in 24 hours after infection in sour orange plant by both treatments and it seems that these mentioned inducer of resistance genes involved in citrus blast disease resistance in host plant

Keywords


اشرفی جواد، رهنما کامران، بابایی زاد ولی اله، رمضانپور سیده ساناز، کیل کریستوف (1399) بررسی تاثیر باکتری Pseudomonas protegens CHA0 و قارچ اندوفیت Serendipita indica در القای مقاومت و بیان ژن‎ های دفاعی در گندم بر علیه عامل بیماری‎ زای سپتوریوز برگ. بیماریهای گیاهی 56(3)، 301-275.
آهنگر لیلا، بابایی زاد ولی اله، رنجبر غلامعلی، نجفی زرینی حمید، بیابانی عباس (1394) الگوی بیان ژن های دفاعی ارقام حساس و مقاوم گندم در پاسخ به آلودگی به سفیدک سطحی. ژنتیک نوین 10(1)، 46-33.
بیکی فرید، رحیمیان حشمت اله، محمدی گل تپه ابراهیم، شمس بخش مسعود، برزگر علی، بوسکت آنتونی، گارسیا والدس النا، لالوکات جرج (1391) بررسی فنوتیپی و بیماری‌زایی عوامل بیماری بلاست مرکبات در استان‌های شمال ایران. دانش گیاهپزشکی ایران. 43(2)، 222-211.
حاجی‌پور باقری عباس، سوهانی محمد مهدی، حسنی سید حسن، بابایی زاد ولی‌اله، علوی سید محمد (1394) اثر همزیستی قارچ Piriformospora indica بر واکنش گیاه برنج (Oryza sativa) به بیماری پوسیدگی طوقه. تحقیقات غلات 5(3)، 230-219.
حیدری نژاد امیر مسعود، بابایی زاد ولی اله، رحیمیان حشمت اله (1395) مطالعه نقش ژن‌های PR2 و PAL در مقاومت گیاه برنج به باکتری Acidovorax avenae subsp. avenae. مجله بیوتکنولوژی کشاورزی7(4)، 82-67.
خاکساری مهسا، بابایی زاد ولی اله، رحیمیان حشمت اله، بیگی فرید (1396) بررسی الگوی بیان چند ژن مقاومت در سه ژنوتیپ مرکبات در برابر باکتری عامل بلاست Pseudomonas syringae pv. syringae.‎ ژنتیک نوین 12(3)، 10-1.
رستمی مهدی، طریقی سعید، طاهری پریسا، رحیمیان حشمت اله (1398) بیان ژن های کیتیناز، بتا-1 و 3 گلوکوناز و پراکسیداز در برنج تحت تیمارهای قارچ عامل بیماری. آفات و بیماریهای گیاهی 87(1)، 146-129.‎
سیاری محمد، بابایی زاد ولی اله،, تاجیک قنبری محمد علی، رحیمیان حشمت اله (1394) بررسی مقاومت دو رقم برنج در تعامل با قارچ عامل سوختگی غلاف، Rhizoctonia solani . مجله بیوتکنولوژی کشاورزی 7(4)، 112-97.‎
صادقی فاطمه، رحیمیان حشمت اله، بابایی زاد ولی اله (1390) تنوع ژنتیکی جدایه های Pseudomonas viridiflava عامل بیماری بلاست مرکبات با آغازگر gyr B. هفتمین همایش بیوتکنولوژی جمهوری اسلامی ایران 37.
عرب پور رق آبادی زهرا، محمدآبادی محمدرضا، خضری امین (1400) الگوی بیانی ژن p32 در بافت‌های ران، دست، راسته و چربی پشت بره کرمانی. مجله بیوتکنولوژی کشاورزی، 13(4)، 183-200.
غلام نژاد جلال، سنجریان فروغ، محمدی گل تپه ابراهیم، صفایی ناصر، رضوی خدیجه (1395) بررسی تغییرات بیان ژن های دفاعی گیاه گندم در پاسخ به آلودگی به Mycosphaerella graminicola. زیست شناسی گیاهی 8(30)، 54-43.
محمدآبادی محمدرضا (1399) بیان ژن ESR1 در بز کرکی راینی با استفاده از real time PCR‎. مجله بیوتکنولوژی کشاورزی 12(1)، 192-177.
محمدآبادی محمدرضا (1399) پروفایل بیانیmRNA مختص بافت ژن ESR2 در بز. مجله بیوتکنولوژی کشاورزی 12(4)، 184-169.
محمدآبادی محمدرضا، سفلایی محمد (1399). پروفایل بیانی mRNA مختص بافت ژن BMP15 در بز. مجله بیوتکنولوژی کشاورزی 12(3)، 208-191.
محمدآبادی محمدرضا، کرد محبوبه، نظری محمود (1397) مطالعه بیان ژن لپتین در بافت‌های مختلف گوسفند کرمانی با استفاده از real time PCR. مجله بیوتکنولوژی کشاورزی 10(3)، 122-111.
منصوری مهدی، حسینی پور اکبر، شریفی سیرچی غلامرضا، معصومی حسین (1388) بررسی تغییرات رونوشت های دو ژن کیتیناز و بتا 1 و 3 گلوکاناز در نهال های پرتقال (Citrus sinensis)در پاسخ به آلودگی باکتری عامل شانکر مرکبات. مجله بیوتکنولوژی کشاورزی 1(2)، 52-39.
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