Investigation of genetic diversity and study of population structure in Aegilops species with U genome by CBDP markers

Document Type : Research Paper

Authors

1 Post Graduated Ph.D. student, Deptartment of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. In addition, Researcher, Seed and Plant Improvement Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

2 Corresponding author. Professors, Deptartment of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

3 Assistant Professors, Seed and Plant Improvement Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

4 Professors, Deptartment of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Aegilops species possessing the U genome are the most widely distributed species in the world. Considering the limitation in the genetic diversity in cultivated wheat, the use of wild relatives and other species of Aegilops can be provided a rich and diverse gene pool of new and ideal alleles for breeders. Therefore, the main goals of the present study were investigation of genetic diversity and population structure in Aegilops accessions collected from different regions of Iran using the CBDP markers.
Materials and methods
In this study, the genetic diversity among 77 Aegilops accessions collected from 18 provinces in Iran and belonging to five species including Ae. biuncialis (UUMM genome), Ae. columnaris (UUMM genome) Ae. Neglecta (UUMM genome), Ae. triuncialis (UUCC genome), Ae. umbellulata (UU genome) , was evaluated using 15 CBDP primers. The obtained molecular data were subjected to statistical analyses using GenAlEx ver. 6.502, DARwin ver. 6, and Structure ver. 2.3.4 softwares.
Results
A total of 189 polymorphic fragments were amplified using 15 used CBDP primers (95.27%). The PIC index ranged from 0.28 (CBDP15) to 0.42 (CBDP-2 CBDP-4) with an average of 0.35. The results of analysis of molecular variance (AMOVA) revealed that the highest proportion of genetic variance referred to within species (76%). Among all species, Ae. triuncialis showed the highest values of genetic parameters. The highest level of genetic similarity was found between Ae. biuncialis with Ae. columnaris (0.905) and Ae.biuncialis with Ae. neglecta (0.879). Although cluster analysis based on CBDP data classified all accessions into three main groups the grouping pattern was exactly in accordance with the genomic constitution of species. Moreover, the clustering pattern was confirmed by a principal coordinate’s analysis (PCoA). The population structure analysis further confirmed the results obtained from the cluster analysis and PCoA, so all studied accessions were grouped based on their genomic structure, degree of genetic similarity, and geographic similarities.
Conclusion
Results of the present study showed a high usefulness of CBDP markers in evaluating the genetic diversity in the Aegilops germplasm. Therefore, it seems that this marker technique can be used in programs related to the preparation of genetic maps and molecular phylogenetic studies. Also, the existence of high genetic diversity among some Iranian Aegilops species can provides a significant prospect for breeders to use them in pre-breeding programs in wheat.

Keywords

References

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Agricultural Biotechnology Journal
Volume 16, Issue 2
June 2024
Pages 69-90

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