Antibacterial Activity of Silver Nanoparticles Synthesized by Hexane Extract of some freshwater algae Against multi-Drug Resistance Bacteria

Document Type : Research Paper

Authors

Department of Biology, College of Science, University of Thi-Qar, Thi-Qar, 64001, Iraq

Abstract

Objectives
Antibiotic-resistant bacteria have become a worldwide concern due to the unintentional use of antibiotics, which has resulted in bacterial strains resistant to many or all available antibiotics. The primary and secondary metabolites found in algae play a major role in the conversion of silver nitrate to silver nanoparticles (AgNPs).
 
Materials and methods
Hexane extract of some freshwater algae was used in the process of making these nanoparticles. The reaction solution's color changing from yellow to dark brown due to the surface plasmon resonance's excitation serves as evidence for this. AgNPs were identified using UV-Vis spectroscopy, proteins and phenols were found to play a significant role in the formation of AgNPs, according to research done using Fourier Transformation-infrared (FTIR) to identify the effective algae group that contributes to the formation of those nanoparticles. A scanning electron microscope (SEM) was used to characterize the shapes and sizes of the synthesized AgNPs, which included spherical, rod-like, and hexagonal structures. Vitek Compact 2 system-diagnosed Multi-Drug Resistant (MDR) bacteria were used to test AgNPs' antibacterial activity.
 
 
 
Results
A study was conducted on the antibacterial effectiveness of biosynthetic silver nanoparticles against selected isolates of MDR bacteria. The results showed that silver nanoparticles prepared from hexane extract of the isolated algae at a concentration of 100% showed greater inhibition than crude extract of all types of pathogenic bacteria, with statistically significant differences (P<0.05).
 
Conclusions
The silver nanoparticles prepared from hexane extract was more effective against G-ve and G+Ve MDR bacterial isolates (E. coli, P. aeruginosa, S. aureus, K. Pneumoniae, and E. faecalis) at concentrations 100 μg/mL than those prepared without silver nanoparticles hexane extract, the extract from Cladophora neglecta silver nanoparticles demonstrated highly significant inhibition in all species of bacteria.

Keywords


Abd El Aty A, Alharbia R, Soliman A (2020) Comparative study on biosynthesis of valuable antimicrobial and antitumor nano-silver using fresh water green and blue-green microalgae. J Microbiol Biotechnol Food Sci 10(2), 249-256.‏
Ali WAA, Taher ZH (2023) Attitudes and Commitment of Healthcare Workers toward Methicillin-resistant Staphylococcus Aureus (MRSA) Infections in Hospitals of Thi-Qar Governorate. University of Thi-Qar J Sci 10(2), 60-67.‏
Alnuaimi M, Hamdan N, Al-Rheem E, Al-Janabi Z (2019) Biodegradation of malathion pesticide by silver bio nanoparticles of Bacillus licheniformis extracts. Res Crops 20, S79-S84.
Bērziņš K, Fraser-Miller SJ, Gordon KC (2021) Recent advances in low-frequency Raman spectroscopy for pharmaceutical applications. Int J Pharm 592, e120034.‏
Bhuyar P, Rahim M, Sundararaju S, et al. (2020) Synthesis of silver nanoparticles using marine macroalgae Padina sp. and its antibacterial activity towards pathogenic bacteria. Beni-Suef Uni J Basic Applied Sci 9(1), 1-15.
Biliuk AA, Semchuk OY, Havryliuk OO (2020) Width of the surface plasmon resonance line in spherical metal nanoparticles. Semicond Phys Quantum Electron optoelectron  23(3), 308-315.
Boucher HW (2020) Bad bugs, no drugs 2002–2020: progress, challenges, and call to action. Trans Am Clin Climatol Assoc 131, 65-71.‏
Butova VV, Zdravkova VR, Burachevskaia OA, et al. (2023) In Situ FTIR Spectroscopy for Scanning Accessible Active Sites in Defect-Engineered UiO-66. Nanomater 13(10), 1-16.‏
Contreras Quiñones HJ, Lizardo Aguayo DA, Andrade Ortega JA, et al. (2022) Preliminary identification of woods from Mexican pines by ATR-FTIR spectroscopy. Rev Mex Cien For 13(72), 4-29.‏
Drummer S, Madzimbamuto T, Chowdhury M (2021) Green synthesis of transition-metal nanoparticles and their oxides: a review. Materials 14(11), 1-30.
Fatimah I, Hidayat H, Nugroho B, Husein S (2023) Green synthesis of silver nanoparticles using Datura metal flower extract assisted by ultrasound method and its antibacterial activity. Recent Pat Nanotechnol 17(1), 68-73.‏
Ghajiri H, Amal AG, Saadon H, Al-Mankhee AA (2023) Isolating and Identifying Some Types of Bacteria and Fungi from wheat grains and studying the effect of some types of antibiotics and the aqueous and alcoholic extract of the Alium sativum plant on them. Uni Thi-Qar J Sci 10(2), 211-215.‏
Habibullah G, Viktorova J, Ulbrich P, Ruml T (2022) Effect of the physicochemical changes in the antimicrobial durability of green synthesized silver nanoparticles during their long-term storage. RSC Adv 12(47), 30386-30403.‏
Hamdani SS, Bhat BA, Tariq L, et al. (2020) Antibiotic resistance: the future disaster. Int J Res Appl Sci Biotechnol 7(4), 133-145.‏
Heidarpour F, Mohammadabadi MR, Zaidul ISM, et al. (2011) Use of prebiotics in oral delivery of bioactive compounds: a nanotechnology perspective. Pharmazie 66 (5), 319-324.
Hernández-González JC, Martínez-Tapia A, Lazcano-Hernández G, et al. (2021) Bacteriocins from lactic acid bacteria. A powerful alternative as antimicrobials, probiotics, and immunomodulators in veterinary medicine. Animals 11(4), 1-17.‏
Hlail AT (2023) The Most Prescribed Antibiotics for Urinary Tract Infections in Pregnant Women in Nasiriya City-South of Iraq. University of Thi-Qar J of Sci 10(2), 122-125.‏
Holder CF, Schaak RE (2019) Tutorial on powder X-ray diffraction for characterizing nanoscale materials. Acs Nano 13(7), 7359-7365.‏
Islam M, Yesmin R, Ali H, et al. (2018) Antineoplastic Properties of Photo-synthesized Silver Nanoparticles from Hibiscus Sabdariffa Linn. Bark Extract. Cen Asian J Med Sci 4(4), 281-292.‏
Joudah R, Hamim S (2023) Molecular characterization of Klebsiella pneumoniae associated with Thalassemia in Thi-Qar Governorate. Uni Thi-Qar J Sci 10(1), 158-161.‏
Khoshnamvand M, Ashtiani S, Chen Y, Liu J (2020) Impacts of organic matter on the toxicity of biosynthesized silver nanoparticles to green microalgae Chlorella vulgaris. Environ Res 185, e109433.‏
Kusumaningrum HP, Zainuri M, Haryanti WDU, et al. (2019) Formation of Eco-friendly Silver Nanoparticle Microalgae using Chlorella vulgaris. Indones J Mar Sci /Ilmu Kelaut 24(1), 7-14.‏
Li C, Zhang X, Ye T, Li X, Wang G (2022) Protection and Damage Repair Mechanisms Contributed To the Survival of Chroococcidiopsis sp. Exposed To a Mars-Like Near Space Environment. Microbiol Spectr 10(6), e03440-22.
Liu X, Liang Q, Zhang X, et al. (2023) Nano-kirigami enabled chiral nano-cilia with enhanced circular dichroism at visible wavelengths. Nanophotonics 12(8), 1459-1468.‏
Lok CN, Ho CM, Chen R, et al. (2006) Proteomic analysis of the mode of antibacterial action of silver nanoparticles. J Proteome Res 5(4), 916-924.‏
Lyonnais S, Hénaut M, Neyret A, et al. (2021) Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 visions. Sci Rep 11(1), e11885.‏
Mar AA, Kyaw MT, Oo W L, Thaw MM (2018) Applications of silver nanoparticles and zinc oxide nanoparticles from Spirulina platensis JARC-YU 7(1 & 2), 179-193.
Mohammadabadi MR, El-Tamimy M, R Gianello, MR Mozafari (2009) Supramolecular assemblies of zwitterionic nanoliposome-polynucleotide complexes as gene transfer vectors: Nanolipoplex formulation and in vitro characterization. J Liposome Res 19 (2), 105-115.
Mohammadabadi MR, Mozafari MR (2018) Enhanced efficacy and bioavailability of thymoquinone using nanoliposomal dosage form. J Drug Deliv Sci Technol 47 (1), 445–453.
Mohammadabadi MR, Mozafari MR (2019) Development of nanoliposome-encapsulated thymoquinone: evaluation of loading efficiency and particle characterization. J Biopharm 11(4), 39-46
Morris S, Cerceo E (2020) Trends, epidemiology, and management of multi-drug resistant gram-negative bacterial infections in the hospitalized setting. Antibiotics 9(4), 196, 1-20.‏
Mortazavi SM, Mohammadabadi MR, MR Mozafari (2005) Applications and in vivo behavior of lipid vesicles. Nanoliposomes From Fund to Recent Dev, 67-76.
Panzarini E, Mariano S, Carata E, et al. (2018) Intracellular Transport of Silver and Gold Nanoparticles and Biological Responses: An Update. Int J Mol Sci 19(5), e1305.
Putra AR, Effendi MH, Koesdarto S, et al. (2020) Detection of the extended spectrum β- lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia. Iraqi J Vet Sci 34(1), 203-207.
Semchuk OY, Biliuk AA, Havryliuk OO, Biliuk AI (2021) Kinetic theory of electro conductivity of metal nanoparticles in the condition of surface Plasmon resonance. Appl Surf Sci Adv 3, 100057, 1-6.
Shareef AA, Farhan FJ, Alriyahee FAA (2024) Green Synthesis of Silver Nanoparticles Using Aqueous Extract of Typha domingensis Pers. Pollen (Qurraid) and Evaluate its Antibacterial Activity. Baghdad Sci J 21(1), 28-40.
Shirley B, Jarochowska E (2022) Chemical characterization is rough: the impact of topography and measurement parameters on energy-dispersive X-ray spectroscopy in bio minerals. Facies 68(2), 1-15.
Singh S, Aswath MU, Biswas RD, et al. (2019) Role of iron in the enhanced reactivity of pulverized red mud: analysis by Mössbauer spectroscopy and FTIR spectroscopy. Case Stud Constr Mater 11, e00266.
Vivas R, Barbosa AAT, Dolabela SS, Jain S (2019) Multidrug-resistant bacteria and alternative methods to control them: an overview. Microbial Drug Resis 25(6), 890-908.‏
Yadi M, Mostafavi E, Saleh B, et al. (2018) Current developments in green synthesis of metallic nanoparticles using plant extracts: a review. Artif Cells, Nano Med Biotechnol 46(sup3), 336-343.‏
Yu C, Tang J, Liu X, et al. (2019) Green biosynthesis of silver nanoparticles using Eriobotrya japonica (Thunb.) leaf extract for reductive catalysis. Materials 12(1), e189.
Zarrabi A, Alipoor Amro Abadi M, Khorasani S, et al. (2020) Nanoliposomes and Tocosomes as Multifunctional Nanocarriers for the Encapsulation of Nutraceutical and Dietary Molecules. Molecules 25 (3), e638.
Zhao R, Xiang J, Wang B, et al. (2022) Recent advances in the development of noble metal NPs for cancer therapy. Bioinorg Chem Appl 2022(1), 1-14.