Measuring the relative expression pattern of the UCP2 gene in different tissues of the Raini Cashmere goat

Document Type : Research Paper

Authors

1 Professor of Animal Science Department, Shahid Bahonar University of Kerman

2 Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine.

3 Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine

4 Sumy National Agrarian University, Sumy, Ukraine

5 Associate professor, Department of Technologies of Livestock Production and processing, Higher Educational Institution “Podillia State University”, Ukraine

6 Associate Professor, Department of Technologies of Livestock Production and processing, Higher Educational Institution “Podillia State University”, Ukraine

Abstract

Objective
The Raini cashmere goat is one of the most momentous Iranian goat breeds. These animals are bred for production of not only cashmere but also meat. Factors such as lipid peroxidation, oxidative stress, and changes in membrane properties are among the factors that cause the death of germ cells at different stages of development and ultimately have a negative effect on production and reproduction. To investigate this process, the UCP2 gene, a protein from the inner membrane of the mitochondrial organelle that pertains to uncoupling proteins family that performs a dominant function in reducing the membrane potential of the mitochondrial organelle and the loss of metabolic energy by preventing the accumulation of oxidative stress, was studied. Moreover, this gene is implicated in the physiology of several tissues, and the dominant isoform of its unconjugated proteins performs a dominant function in preventing inflammation and inhibiting cell death. The goal of the present study was therefore to consider the relative expression level of the UCP2 gene in several tissues of the Raini cashmere goat applying the Real-Time PCR technique.
Materials and Methods
For this purpose, samples of humeral muscle, back muscle, femur muscle, spleen, heart, testis, liver and back fat tissues (three replicates of each tissue) were collected from four Raini cashmere goats of approximately the same weight at the time of slaughter. Total RNA of these tissues was extracted and their cDNA amplified. Real-Time PCR reaction using the non-specific SYBR Green dye method was performed to examine the relative gene expression. In this study, the GAPDH gene was used as an internal control. Prism software was applied to analyze the raw data, analyze the data got from Real-Time PCR and calculate the extent of changes in gene expression.
Results
The results of current investigation showed that the UCP2 gene was expressed in all tissues examined, with the highest expression level in the humeral muscle tissue (9.5-fold) and back muscle (4.5-fold), while the lowest expression level was observed in the back fat tissue (1.3-fold) and liver (1.5-fold) (P<0.01).
Conclusions
Considering that the UCP2 gene was expressed in all tissues examined and showed different expression levels in the different tissues, it could be inferred that this gene performs a function in different mechanisms and activities. Therefore, by conducting further experiments and understanding the relevant mechanisms, the cause of the change in expression can be found and action can be taken to improve livestock performance by changing the expression of this gene.  

Keywords

References

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Agricultural Biotechnology Journal
Volume 16, Issue 3
September 2024
Pages 317-332

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