Genetic and phylogenetic analysis of cytochrome b region in Jebeer of Iran

Document Type : Research Paper

Authors

Abstract

Jebeer is belonging to subfamily of mountain goats (Antilopinae), genus of Gazelle (Gazella) and found mainly in central and southern desert areas of Iran. There are many challenges between researchers about determining Jebeer species. Whereas determining Jebeer species is first step in protecting of them we perform this research. In this regard 5 biological samples from Shirahmad’s wild life refuge of Sabzevar were collected and cytochrome b region of mitochondrial DNA have been amplified with L14724 and H15915 primers. Resulting fragments were sequenced with ABI 3130 automated device with Sanger method. These sequences compared with 190 sequences from Gazella genus that classified according to species with regard to homology and genetic distances, then phylogenetic tree have been drawn. Results show that among all Gazelle sp. Jebeer have least distance equal to 0.003 with G. bennetti  and most distance with G. dorca and his sister taxon G. saudiya that is equal to 0.073 and 0.074 respectively.  Thereby can conclude that Jebeer is belonging to G. bennetti species not G. dorcas

Keywords

Full Text

Jebeer, Cytochrome b, Mitochondrial DNA, Gazella bennetti.

 

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Agricultural Biotechnology Journal
Volume 3, Issue 1 - Serial Number 1
vol 3.No1 spring and summer 1390
June 2011
Pages 91-104

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