Effect of DL- Methionine Replacement with L- Methionine and Different Dietary Protein Levels on Myostatin Gene Expression in Japanese Quails

Document Type : Research Paper

Authors

1 MS graduate of Animal genetic and Breeding, Faculty of Animal Science and Food Technology, Agricultural science and Natural Resources University of Khuzestan, Iran

2 Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

3 Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Iran

4 Assistant Professor, Department of Animal Science, Faculty of Animal science and Food Technology, Agricultural Science and Natural Resources University of Khuzestan, Ahvaz, Iran

Abstract

Objective
Myostatin also known as growth differentiation factor 8, is a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. This research had been done in order to assess the effect of DL-methionine replacement with L-methionine, and also the effects of varying dietary protein levels on Myostatin gene expression in Japanese quail.
 
Materials and methods
This experiment was carried in the form of a 2×2 factorial with 4 treatment. Treatments contained the replacement of DL-methionine with L- methionine, and different dietary protein levels, were 24 and 20 %. After about 35 days of feeding and keeping the quails, a piece of their chest has been removed immediately and were transferred to the laboratory. Myostatin gene expression measured by using RT-qPCR technique. In this method, 𝜷-actin gene was used as a house-keeping gene to normalize the gene expression data in the quantitative real time PCR.
 
Results
DL-methionine replacement did not significantly effect on Myostatin gene expression. Whereas, reduction in protein surface from 24% to 20 %, led to significantly increased expression of Myostatin (P<0.01).
 
Conclusions
The results indicated that DL methionine could be replaced with L methionine, and the appropriate level of protein was 24% in the Japanese quail diet.

Keywords


آقائی علی، خسروی نیا حشمت اله، مموئی مرتضی و همکاران (1397) اثر مکمل عنصر روی و ویتامینE بر آنزیم های آنتی اکسیدان، هورمون های جنسی و برخی فراسنجه های بیوشیمیایی در گله های مادر بلدرچین ژاپنی. مجله دامپزشکی ایران 14(2)، 14-24.
توحیدی نژاد فاطمه، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، نجمی نوری عذرا (1393) مقایسه سطوح مختلف بیان ژنRheb  در بافت های مختلف بز کرکی راینی. مجله بیوتکنولوژی کشاورزی 6(4)، 50-35.
جعفری دره در امیر حسین، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، ریاحی مدوار علی (1395) بررسی بیان ژن CIB4  در بافت­های مختلف گوسفند کرمانی با استفاده از Real Time qPCR. مجله پژوهش در نشخوارکنندگان 4(4)، 132-119.
رستم زاده الهه، اسدی فوزی مسعود، اسمعیلی زاده کشکوئیه علی، اسدی ملک حسین (1395) بررسی اثر محدودیت متیونین بر بیان ژن IGF-1 در عضله سینه بلدرچین ژاپنی. مجله بیوتکنولوژی کشاورزی 8(1)، 60-47.
          محمدی فر آمنه، فقیه ایمانی سید علی، محمدآبادی محمدرضا، سفلایی محمد (1392) تأثیر ژن TGFb3 بر ارزش های فنوتیپی و ارثی صفات وزن بدن در مرغ بومی استان فارس. مجله بیوتکنولوژی کشاورزی 5(4)، 125-136.
صوفی بهاره، محمدآبادی محمدرضا، شجاعیان کمال و همکاران (1388) ارزیابی چند شکلی ژن میوستاتین در گوسفند نژاد سنجابی با استفاده از روش PCR-RFLP . مجله پژوهشهای علوم دامی 19(1)، 81-89.
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