Studying expression of leptin gene in different tissues of Kermani Sheep using Real Time PCR

Document Type : Research Paper

Authors

1 Department of Animal Science, Faculty of Agriculture, College of Agriculture, Shahid Bahonar University of Kerman

2 MSc Student, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.

3 Assistant Professor, Department of Animal Science, Ramin Agricultural & Natural Resources University of Khuzestan, Ahvaz, Iran.

Abstract

Objective
Leptin is produced by white adipose tissue and plays an important role in regulation of feed intake, energy balance, fertility and immune functions. Thus, the aim of this research was to study leptin gene expression in adipose tissue, liver, kidney, lung and heart of Kermani sheep.
 
Materials and methods
Tissue samples obtained from 6 animals and RNA was extracted. Extracted RNA were immediately stored at -80°C. Quality and quantity of RNA were evaluated and cDNA was synthesized and Real Time PCR was performed. PCR Products were electrophoresed on 1.5% agarose gel and were evaluated different levels of expression in studied different tissues.
 
  
Results
Results showed that the leptin gene was expressed in all the tested tissues and the highest level of expression was observed in adipose tissue and liver and the lowest level was detected in heart.
 
Conclusions
These results may show that leptin plays a particular role in fat metabolism. Further studies are needed to clarify role of leptin in the physiology of fat metabolism and other materials. This would help us to better understand the mechanisms for the known effect of nutritional factors and body fatness on various functions.

Keywords

References

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Agricultural Biotechnology Journal
Volume 10, Issue 3 - Serial Number 31
Volume 10, No 3
October 2018
Pages 111-123

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