Evaluation of genetic diversity among Iranian apple cultivars using ISSR-markers

Document Type : Research Paper

Authors

1 Graduated MSc student, Department of Horticultural Biotechnology, Islamic Azad University, Jiroft Branch, Jiroft, Iran.

2 Assistant Professor, Department of Plant Pathology, Islamic Azad University, Rafsanjan Branch, Rafsanjan, Iran and Young Researchers and Elite Club, Rafsanjan Branch, Islamic Azad University, Rafsanjan, Iran.

Abstract

Objective
Apple (Malusdomestica Borkh) from Rosaceae family is one of the most important fruit trees in the world. Evaluation of genetic diversity is one of the basic steps for plant breeding and preserving of germ plasm through gene banks.
 
Materials and methods
The aim of this study was evaluation of the genetic diversity of 22 varieties of apples collected from Khorasan Aagricultural Research Center and some region of Jiroft using ISSR markers based on the polymerase chain reaction (PCR).
 
Materials and methods
DNA extraction was done and then PCR conducted by five ISSR-primers.
 
Results
Apple cultivars had 81.48% polymorphism. Cluster analysis classified them into six groups. The first group had five apple cultivars from Khorasan Razavi including Mohali Gonabad, Tabasi number3, Ghasemabai, Mohamadi and Golshahi Oghaz as well as Golab Esfehan, Sibe Ghermez and Gerani Esmith from Jiroft. Genotypes of Zard Lobnan, Sib Ghermez Khoni, Mohali Sardo, Golab Sardo from Jiroft and Mashali, Morabei Mashhad, Ali Mori Dovom Ras, Arbabi, SIB number3 from Khorasan Razavi categorized into the second group. Genotypes of Shaikh Ahmad Tabriz and Shafiabadi were placed in the third and forth group respectively. The fifth and sixth groups include Gol Mashhad and Abasi Azghade, Atlasi Tabas from Khorasan Razavi. Also, ISSR primer 842 showed the highest polymorphism among apple cultivars. There is no correlation between the geographical origin and genetic grouping of apple cultivars. Probably, setting of different regions genotypes in the same genetic group is due to transport of genotypes among regions in past.
 
Conclusions
Results indicated that ISSR is an effective technique for identification of polymorphism, genetic distance and germplasm management among apple cultivars.

Keywords

References

منابع
ارجمند قهستانی رامین، توسلیان ایرج، محمدی نژاد قاسم (1394) ارزیابی تنوع ژنتیکی25 ژنوتیپ پسته ایرانی با استفاده از نشانگر مولکولی ISSR. مجله بیوتکنولوژی کشاورزی 7(3)، 18-1.
بهادر یاسر ، محمدآبادی محمدرضا، خضری امین ، اسدی مهدیه، مدحتی لیلا (1395) مطالعه تنوع ژنتیکی جمعیت های زنبور عسل استان کرمان با استفاده از نشان­گرهای ISSR. 7(13)، 56-49.
صفرپور شورباخلو مریم، حسینی منفرد روح اله، پایدار سولماز، مستانه شریفی (1394) تعیین تنوع ژنتیکی ارقام گلابی با استفاده از نشانگرهای ISSR. 7(1)، 132-115.

عسکری ناهید ، باقی زاده امین ، محمدآبادی محمدرضا (1389) بررسی تنوع ژنتیکی در چهار جمعیت بز کرکی رائینی با استفاده از لوکوس های بین ریزماهواره (ISSR). 5(2)، 56-49.

کشاورزخوب محمدقاسم، قرنجیک شاهرخ، معصومی اصل اسد، عبدالهی مندولکانی بابک (1394) بررسی تنوع و روابط ژنتیکی برخی از ژنوتیپ‌های انگور (Vitis vinifera L) با استفاده از نشانگرهای مولکولی ISSR. 7(4)، 142-129.
 
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Agricultural Biotechnology Journal
Volume 10, Issue 4 - Serial Number 32
Volume 10, No 4
December 2018
Pages 35-53

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