Investigation of Genetic Variation in Cumin (Cuminum cyminum) Ecotypes of Khorasan Provinces using RAPD and ISSR Markers

Document Type : Research Paper

Authors

1 agronomy-agriculture-university of birjand

2 agronomy

Abstract

Objective
Determination of genetic variation in plant material is an initial step for identification, preservation and maintenance of heritable reserves as well as the basis for genetic research and breeding programs. The aim of this research was to study the genetic diversity and categorization of cumin ecotypes collected from North, Razavi and South Khorasan provinces based on RAPD and ISSR markers. Ultimately, the aim was to answer the following questions: Is there a genetic diversity between cumin populations and is there a correlation between the genetic diversity and geographic diversity of cumin ecotypes?
 


Materials and methods
In this research, 20 different cumin ecotypes were cultivated for DNA extraction in the College of Agriculture of university of Birjand. The quantity and quality of the extracted DNA were evaluated using Nano drop and 1% agarose gel electrophoresis. For genetic variation, nine primers of RAPD and 30 ISSR primers were used.
 
Results
The results of the analysis of molecular variance (AMOVA) showed that there were variations between and within populations. Based on the RAPD and ISSR markers, 10 and 9 percent of the variation were between populations and 90 and 91 percent were within populations, respectively. Based on the RAPD and ISSR markers the percentage of polymorphism 62% and 70%, the mean of polymorphic content was 0.26 and 0.3, the mean of Shannon's information index was 1.37 and 1.9, and the mean of the information index 2.1 and 2.76 were obtained, respectively. The results PCoA showed that for the RAPD marker, the first two components were accounted 40.4% and for ISSR marker, however, the first four components were accounted for 49.6% of the variation. The cluster analysis based on the similarity matrix grouped the ecotypes in 6 and 7 clusters, respectively.
        
Conclusions
According to the obtained information, such as the polymorphism, the large amplitude between the similarity coefficients (jacquard) and the grouping of ecotypes in distinct clusters, there is a genetic diversity between the studied cumin ecotypes. The other hand, in most cases, there was the correlation between the genetic variation in the collected ecotypes and geographic location.
 
 
Zabet M, Rahimi A, Izanlo A, Alizadeh Z (2019) Investigation of Genetic Variation in Cumin (Cuminum cyminum) Ecotypes of Khorasan Provinces using RAPD and ISSR Markers. Agricultural Biotechnology Journal 11 (1), 75-98. 
 
Agricultural Biotechnology Journal 11 (1), 75-98.
10.22103/jab.2019.12102.1050
Received:  October 7, 2018; Accepted: April 8, 2019
© Faculty of Agriculture, Shahid Bahonar University of Kerman-Iranian Biotechnology Society

Keywords


باقری عبدالرضا، محمودی علی‌اکبر  (1381) زیره سبز، فناوری تولید و فرآوری. انتشارات دانشگاه فردوسی مشهد.
بهادر یاسر ، محمدآبادی محمدرضا، خضری امین و همکاران (1395) مطالعه تنوع ژنتیکی جمعیت های زنبور عسل استان کرمان با استفاده از نشان­گرهای ISSR. 7(13)، 56-49.
عسکری ناهید ، باقی زاده امین ، محمدآبادی محمدرضا (1389) بررسی تنوع ژنتیکی در چهار جمعیت بز کرکی رائینی با استفاده از لوکوس های بین ریزماهواره (ISSR). 5(2)، 56-49.
فارسی محمد، باقری عبدالرضا (1388) اصول اصلاح نباتات. انتشارات جهاد دانشگاهی مشهد.
نقوی محمدرضا، قره یاضی بهزاد، حسینی سالکده قاسم (1392) نشانگرهای مولکولی. موسسه انتشارات و چاپ دانشگاه تهران. تهران.
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