A study of possibility of expression of an alternasucrase gene in sugar beet to produce alternan biopolymer

Document Type : Research Paper

Authors

Agronomy and plant Breeding Department, Faculty of Agriculture, Lorestan University, Khorramabad

Abstract

Objective
Lactic acid bacteria (LAB) synthesize valuable industrial and pharmaceutical important biopolymers, such as Alternan, by expressing glycosyltransferase enzymes by utilizing extracellular sucrose. In this study, due to the importance of such versatile biopolymers in the industry and medicine, a gene encoding an Altranansucrase (Asr) was introduced to sugar beet plants.
 
Materials and methods
A gene encoding the Asr gene was isolated from Leuconostoc mesenteroides bacterium and introduced to sugar beet plants by using Agrobacterium-mediated transformation. Molecular techniques were used to analyse transgenic plants and sugar content of sugar beet lines.
 
Results
Out of 131 transformed explants, only three transgenic plants were produced, showing a transformation efficiency of 2.3%. The Asr gene integration in transgenic plants genome and expression were confirmed by specific PCR and semi-quantitative RT-PCR analysis, respectively. Sugar analysis of sugar beet transgenic plants showed that control plants with a 19.6% brix value (Sucrose) had more sucrose than transgenic plants with an average brix value of 14.4%. Brix in transgenic plants was lower than that of control plants. The amount of sugar (sucrose) in the transgenic asr-expressing plants reduced by 36.1% relative to the untransformed control plants.
 
Conclusions
The results of this study showed that Altrenansucrase can convert substantial amount of sugar beet sucrose into Alternan biopolymer, producing 36.6 mg/g FW alternan biopolymer with pharmaceutical and industrial applications.

Keywords


خادمی میترا، نظریان فیروزآبادی فرهاد (1392) تاثیر دو نوع هورمون بر تولید برگ های حاوی پایه جوانه در روش باززایی مستقیم گیاه چغندرقند. فن­آوری زیستی در کشاورزی 4(1)، 52-47.
شرفی، رضا (1392) همسانه­سازی ژن کد­کننده­ی آنزیم موتان سوکراز از باکتری Streptococcus doweni. Mfe 28 برای بیان در گیاه چغندرقند. پایاننامه کارشناسی ارشد، دانشگاه لرستان.
موسوی الهام، نظریان فیروزآبادی فرهاد، اسماعیلی احمد (1395) بررسی طیف آنتی بیوگرام و ژن های مرتبط با بیماری زایی دو سویه انتروکوکوس فاسیوم جداسازی شده از شیره درخت بلوط .(Quercus brantii var. persica)مجله دانشگاه علوم پزشکی مازندران 26(146)، 46-31.
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