Comparative assessment of SCoT and CBDP markers for investigation of genetic diversity existing in different aegilops species

Document Type : Research Paper

Authors

1 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

2 Assistant Professor, Department of Plant Breeding, Kermanshah Brunch, Islamic Azad University, Kermanshah, Iran

3 Department of Plant Breeding, Kermanshah Brunch, Islamic Azad University, Kermanshah, Iran

4 Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, University of Tehran, Tehran, Iran

Abstract

Objective
The main goals of this study were investigation of genetic diversity in 103 Aegilops accessions and comparison of the efficiency of start codon targeted (SCoT) and CAAT box-derived polymorphism (CBDP) markers.
 
          Materials and methods
In this study, the genetic diversity in 103 Aegilops accessions belonging to seven species including seven samples of Ae. caudata, 14 samples of Ae. crassa, 19 samples of Ae. cylindrica, 11 samples of Ae. neglecta, 20 samples of Ae. tauschii, 15 samples of Ae. triuncialis and 17 samples of Ae. umbellulata was evaluated using 15 SCoT and 15 CBDP primers.
 
Results
In total, 15 SCoT and 15 CBDP primers amplified 164 and 141 polymorphic bands, respectively. SCoT primers showed the highest values for all of the informativeness parameters than CBDP primers. However, both molecular markers indicated the same PIC values. The results of analysis of molecular variance (AMOVA) revealed that the highest proportion of genetic variance referred to within species. Among all species, Ae. cylindrica had the highest values of genetic parameters. Although cluster analysis based on each marker system classified all accessions into two main groups, the grouping pattern obtained from CBDP data indicated a clear phylogenetic relationship among Aegilops species compared to SCoT data. Besides, the results of clustering were confirmed by principal coordinates analysis (PCoA) analysis.
 
Conclusion
On the whole, both molecular markers revealed good capability in depicting of polymorphism among tested accessions. However, CBDP markers provided a vivid grouping pattern for evaluated samples. Hence, the use of this technique individually or in combination with other molecular markers is recommended for phylogenetic assessments. 

Keywords


References
Ahmadi J, Pour-Aboughadareh A, Fabriki-Ourang S et al. (2018a) Screening wild progenitors of wheat for salinity stress at early stages of plant growth: insight into potential sources of variability for salinity adaptation in wheat. Crop Pasture Sci 69, 649–58.
Ahmadi J, Pour-Aboughadareh A, Ourang SF et al. (2018b) Wild relatives of wheat: Aegilops–Triticum accessions disclose differential antioxidative and physiological responses to water stress. Acta Physiol Plant 40, 90e.
Altintas S, Toklu F, Kafkas S et al. (2008) Estimating genetic diversity in durum and bread wheat cultivars from Turkey using AFLP and SAMPL markers. Plant Breed 127, 9–14.
Anderson JA, Churchill GA, Autrique JE et al. (1993) Optimizing parental selection for genetic linkage maps. Genome 36, 181–186.
Badaeva E, Amosova A, Samatadze T et al. (2004) Genome differentiation in Aegilops. 4. Evolution of the U-genome cluster. Plant Syst Evol 246, 45–76.
Badfar-Chaleshtori S, Shiran B, Kohgard M et al. (2012) Assessment of genetic diversity and structure of Imperial Crown (Fritillaria imperialis L.) populations in the Zagros region of Iran using AFLP, ISSR and RAPD markers and implications for its conservation. Biochem Syst Ecol  42, 35-48.
Collard BCY, Mackill DJ (2009) Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep 27, 86–93.
Doyle JJ, Doyle KJ (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11–15.
Etminan A, Mehrabi AA, Shooshtari L, Moradkhani H (2018a) Applicability of CBDP markers to study of genetic diversity among some of the cultivated wheat accessions and their ancestral species. Mod. Genet 52, 79–89. (In Persian).
Etminan A, Pour-Aboughadareh A, Mohammadi R et al. (2016) Applicability of start codon targeted (SCoT) and inter-simple sequence repeat (ISSR) markers for genetic diversity analysis in durum wheat genotypes. Biotechol Biotechnol Equi 30, 1075–1081.
Etminan A, Pour-Aboughadareh A, Mohammadi R et al. (2018b) Applicability of CAAT box-derived polymorphism (CBDP) markers for analysis of genetic diversity in durum wheat. Cereal Res Commun 46, 1–9.
Etminan A, Pour-Aboughadareh A, Noori A et al. (2018c) Genetic relationships and diversity among wild Salvia accessions revealed by ISSR and SCoT markers. Biotech Biotechnol Equi 32, 610–617.
Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci Am 244, 98–109.
Hajjar R, Hodgking T (2007) The use of wild relatives in crop improvement: A survey of developments over the last 20 years. Euphytica 156, 1–13.
Hamidi H, Talebi R, Keshavarz F (2014) Comparative efficiency of functional genebased markers, start codon targeted polymorphism (SCoT) and conserved DNA-derived Polymorphism (CDDP) with ISSR markers for diagnostic fingerprinting in wheat (Triticum aestivum L.). Cereal Res Commun 42, 558–567.
Heidari P, Etminan A, Azizinezhad R, Khosroshahli M (2017) Genomic variation studies in durum wheat (Triticum turgidum ssp. durum) using CBDP, SCoT and ISSR markers. Indian J Genet Pl Br 77, 379–386.
Kharestani H, Nasrolah Nejad Qomi AA, Mehrabi AA (2013) Genetic diversity assessment of Einkorn wheat by using microsatellite markers. Journal of Crop Production 6, 1–16 (In Persian).
Kilian B, Ozkan H, Deusch O et al. (2007) Independent wheat B and G genome origins in outcrossing Aegilops progenitor haplotypes. Mol Biol Evol 24, 217–227.
Kumar M, Mishra GP, Singh R et al. (2009) Correspondence of ISSR and RAPD markers for comparative analysis of genetic diversity among different apricot genotypes from cold arid deserts of trans- Himalayas. Physiol Mol Biol Plants 15, 225–236.
Maxted N, Kell S (2009) CWR in crop improvement: To what extent are they used? Crop Wild Relative Newsletter 7, 7–8.
Mohammadi SA, Prasanna BM (2003) Analysis of genetic diversity in crop plants—salient statistical tools and considerations. Crop Sci 43, 1235–1248.
Moradkhani H, Mehrabi AA, Etminan A, Pour-Aboughadareh A (2015) Molecular diversity and phylogeny of Triticum–Aegilops species possessing D genome revealed by SSR and ISSR markers. Plant Breeding and Seed Science 71, 82–95.
Moradkhani H, Pour-Aboughadareh AR, Mehrabi AA, Etminan A (2012) Evaluation of genetic relationships of Triticum-Aegilops species possessing D genome in different ploidy levels using microsatellites. Int J Agri Crop Sci 23, 1746–1751.
Mousavifard SS, Saeidi H, Rahiminejad MR, Shamsadini M (2015) Molecular analysis of diversity of diploid Triticum species in Iran using ISSR markers. Genet Resour Crop Evol 62, 387–394.
Naghavi MR, Hajikram M, Taleei AR, Aghaei MJ (2010) Microsatellite analysis of genetic diversity and population genetic structure of Aegilops tauschii Coss. in northern Iran. Genet Resour Crop Ev 57, 423–430.
Naghavi MR, Maleki M, Alizadeh H et al. (2009) An assessment of genetic diversity in wild diploid wheat Triticum boeoticum from west of Iran using RAPD, AFLP and SSR markers. J Agr Sci Tech 11, 585–598.
Nevo E (1998) Genetic diversity in wild cereals: regional and local studies and their bearing on conservation ex situ and in situ. Genet Resour Crop Ev 45, 355–370.
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol 6, 288–295.
Peng X, JiQ, Fan S et al. (2015) Genetic diversity in populations of the endangered medicinal plant Tetrastigma hemsleyanum revealed by ISSR and SRAP markers: implications for conservation. Genet Resour Crop Ev 62, 1069–1078
Poczai P, Varga I, Laos M et al. (2013) Advances in plant gene-targeted and functional markers: a review. Plant Methods 9, 6.
Pour-Aboughadareh A, Ahmadi J, Mehrabi AA et al. (2017a) Physiological responses to drought stress in wild relatives of wheat: implications for wheat improvement. Acta Physiol Plant 39, 49
Pour-Aboughadareh A, Ahmadi J, Mehrabi AA et al. (2018b) Insight into the genetic variability analysis and relationships among some Aegilops and Triticum species, as genome progenitors of bread wheat, using SCoT markers. Plant Biosys 152, 694–703.
Powell W, Morgante M, Andre C et al. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2, 225–238.
Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98, 107–112.
Qaderi A, Omidi M, Pour-Aboughadareh A et al. (2019) Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes. Ind Crop Prod 130, 237–247.
 Saidi A, Jabalameli Z, Ghalamboran M (2018) Evaluation of genetic diversity of carnation cultivars using CDDP and DAMD markers and morphological traits. The Nucleus 61, 129–135
 Schneider A, Molnar I, Molnar-Lang M (2008) Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163, 1–19.
 Singh AK, Rana MK, Singh S et al. (2014) CAAT box-derived polymorphism (CBDP): a novel promoter-targeted molecular marker for plants. J Plant Biochem Biot 23, 175–183.
 Tamura K, Peterson D, Peterson N et al. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731–2739.
 Tiwari G, Singg R, Singh N et al. (2016) Study of arbitrarily amplified (RAPD and ISSR) and gene targeted (SCoT and CBDP) markers for genetic diversity and population structure in Kalmegh [Andrographis paniculata (Burm.f.) Nees]. Ind Crops Prod 86, 1–11.
 Warschefsky E, Penmetsa RV, Cook DR, von Wettberg EJB (2014) Back to the wilds: tapping evolutionary adaptations for resilient crops through systematic hybridization with crop wild relatives. Am J Bot 101, 1791–1800.
 Weide A, Rieh S, Zeidi M, Conard NJ (2013) Using new morphological criteria to identify domesticated emmer wheat at the aceramic Neolithic site of Chogha Golan (Iran). J Archaeol Sci 57, 109–118.