Detection of fire blight resistant genes in apple trees (Malus domestica) planted in Isfahan province using SCAR and SSR markers

Document Type : Research Paper

Authors

1 Horticulture, Azad university,Shiraz,Iran

2 plant pathology Department, Islamic Azad university of Shiraz, Shiraz, Iran

Abstract

Objective
Plant diseases can be a limiting factor in planting in an area. One of the new methods of producing disease resistant plants is the use of molecular markers. Molecular markers are able to detect and analyze important genes of resistance. Therefore, due to the severity of fire blight, the apple tree germplasm evaluation is necessary, so this research was carried out with the aim of determining resistance genes in some apple genotypes in Isfahan province using SCAR and SSR markers.
 
Materials and methods
Fresh and young leaves of 70 samples of apple trees in Isfahan were collected in early April and DNA was extracted by CTAB method. Then polymerase chain reaction was performed and DNA fragment was detected and DNAs lengths were measured.
 
Results
Results indicated that in all populations, none of the primers have a rare band or a typical band replication in 25 or less than 25% population, and 50 or less than 50% population. Semirom- Hana population had the greatest genetic diversity (Nei diversity index, Shannon, effective and different number of alleles) which shows greater genetic diversity in this population rather than other populations.Molecular analysis indicated that diversity among populations is not statistically significant and 92% of the diversity is related to diversity within the populations. The longest distance was between Semirom-Hana and Semirom-Padena populations, and in this disease, the distance between the farthest and closest populations is too low and this result was confirmed by analysis of molecular variance.
 
Conclusion
The six primers, which were used in this study, have been well produced and display the distinctions in population. Moreover, results indicate that inter-population diversity was very low but by the cross of the farthest and closest populations, then the cross of progeny
can pyramidize the target genes and produce a stronger and more diverse recombination.

Keywords


آگریوس جرج ان  (1389)بیماری شناسی گیاهی. ترجمه ایزدپناه ک.؛ اشکان م؛ بنی هاشمی ض؛ رحیمیان ح؛ میناسیان و ویرایش پنجم، چاپ اول، تهران، انتشارات آییژ.
امیدوار رضا؛ شمس‌بخش مسعود؛ رحیمیان حشمت ا... (1384) تعیین خصوصیات و تفکیک استرین‌های ایرانی باکتری Ervinia amylovora  با استفاده از روش‌های بیوشیمیایی و مولکولی. چهارمین همایش بیوتکنولوژی ایران، ایران، کرمان
دولتی بانه حمید؛ مجدی وحید (1388) ژنتیک مقاومت در مقابل بیماری های سیب. مجموعه گزارش مرکز تحقیقات کشاورزی و منابع طبیعی استان آذربایجان غربی، 23صفحه
بهادر یاسر؛ محمدآبادی محمدرضا؛ خضری امین؛ اسدی مهدیه؛ مدحتی لیلا (1395) مطالعه تنوع ژنتیکی جمعیت‌های زنبور عسل استان کرمان با استفاده از نشانگرهای .ISSR پژوهش‌های تولیدات دامی، سال هفتم، شماره 13، 186-192       .
سایت اداره کل هواشناسی استان اصفهان WWW.esfahanmet.ir
سلمانیان زهرا؛ نماینده آنیتا؛ هنرور مهرزاد )1393 (کاربرد نشانگر‌های مولکولی SSR و SCAR جهت بررسی ژنوتیپ‌های سیب مقاوم به آتشک. همایش ملی الکترونیکی دستاورد‌های نوین در علوم مهندسی و پایه، مرداد 1393، ایران، تهران، مرکز پژوهش‌های زمین کاو.
عسکری ناهید؛ باقی زاده امین؛ محمدآبادی محمدرضا(1389)  بررسی ژنتیکی در چهار جمعیت بز کرکی رائینی با استفاده از لوکوس‌های بین ریزماهواره‌ای (ISSR). ژنتیک نوین، دوره پنجم، شماره 2، 49-56.
ملکی بالا‌جو امید؛ کشاورزی منصوره؛ رضایی دانش یونس؛ دامیار سیما؛ جعفری مراد (1390) واکنش تعدادی از ژنوتیپ‌های کلکسیون سیب بومی ایران به E. amylovora.. مجله به‌نژادی نهال و بذر، 1-27: 23-36.
نقوی محمدرضا. قره یاضی بهزاد. حسینی سالکده قاسم. (1384)  نشانگرهای مولکولی. چاپ اول، تهران، انتشارات دانشگاه تهران.
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