Optimization of tissue culture and transformation of construct contain GDP-mannose-3´, 5´-epimerase (GME) gene from actinidia deliciosa L. into Lactuca sativa L

Document Type : Research Paper

Authors

Department of Production Engineering and Plant Genetics, Faculty of Science and Agricultural Engineering, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran

Abstract

Objective
Lettuce is considered as a model plant for biotechnology because of its compatibility with stable genetic transformation and tissue culture. GDP-mannose-3’,5’-epimerase (GME) is one of the key genes in ascorbic acid biosynthesis pathway in plants. The present study aims to transfer GME gene from Actinidia deliciosa L. into Lactuca sativa L.
 
Materials and Methods
To investigate callus induction rate using the effects of explant (cotyledon and true leaves) and 16 plant growth regulator combination including concentrations of 0.02, 0.04, 0.05, and 0.1 mg/l NAA and 0.1, 0.2, 0.4, and 0.6 mg/l BAP, and also direct regeneration rate using the effects of explant (cotyledon and true leaves) and 6 plant growth regulator combination including concentrations of 0.02 and 0.05 mg/l NAA and 0.2, 0.4, and 0.6 mg/l BAP, a factorial experiment based on completely randomized design with three replications was conducted. In order to transform GME into Lactuca sativa L. using L. sativa L. cv. Setareh and Agrobacterium tumefaciens strain (C58) on two types of explants (cotyledon and true leaves), a factorial experiment with three replications and 2 min and 8 min inoculation was done.
 
Results
The results revealed that the highest percentage of callus induction and indirect regeneration (100%) were observed on leaf and cotyledon explants and MS medium containing 0.1 mg/l BAP and 0.04 mg/l NAA. The results also confirmed the presence of pBI121+GME in transgenic plants.
 
Conclusions
The explant true leaves and 2 min inoculation (with 18 percent transformation ratio) were more suitable for transformation.

Keywords


اکبری لیلا‌‌‍‍‍‍؛ چقامیرزا کیانوش؛ فرشادفر عزت اله (1395) بررسی تحمل به خشکی ژنوتیپ‌های گندم دوروم در شرایط درون شیشه‌ای. مجله پژوهش‌های گیاهی (مجله زیست‌شناسی ایران) 29، 285-273.
محب الدینی مهدی؛ جلالی جواران مختار؛ علیزاده هوشنگ؛ مهبودی فریدون؛ خسروی حسین (1389) بهینه‌سازی روش کشت بافت و سیستم انتقال ژن در گیاه کاهو (Lactuca sativa L.). نشریه علوم باغبانی (علوم و صنایع کشاورزی) 24، 202-195.
نجار خدابخش آزاده؛ چاپارزاده نادر (1394) نقش آسکوربیک اسید در تقلیل اثرات اکسیداتیو شوری روی گیاه شاهی. مجله پژوهش‌های گیاهی 28، 175-185.
هنری حسین؛ علیزاده هوشنگ؛ شاه نجات بوشهری علی اکبر؛ پیغمبری سید علی؛ جلالی جواران مختار؛ براهمیپور روح اله (1391) عوامل موثر در انتقال ژن گزارشگر uidA با استفاده از اگروباکتری به گیاه کاهو (Lactuca sativa L.). مجله علوم باغبانی ایران 43، 91-101.
هنری حسین؛ علیزاده هوشنگ؛ شاه نجات بوشهری علی اکبر؛ پیغمبری سید علی؛ جلالی جواران مختار (1387) باززایی درون شیشه‌ای ارقام کاهوی (Lactuca sativa L.) ایرانی. مجله علوم گیاهان زراعی ایران 39، 173-180.
 
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