Tissue-specific mRNA expression profile of BMP15 gene in goat

Document Type : Research Paper

Authors

1 Professor of Department of Animal Science, Faculty of Agriculture, College of Agriculture, Shahid Bahonar University of Kerman

2 Assistant Professor, Kerman Agriculture and Natural Resources and Education Center, AREEO, Kerman, Iran. Email: soflaei_m@yahoo.com

Abstract

Objective
Three fecundity genes have been identified, namely bone morphogenetic protein receptor type 1B (BMPR1B; or activin-like kinase 6, ALK6) known as FecB on chromosome 6, growth differentiation factor 9 (GDF9) known as FecG on chromosome 5, and bone morphogenetic protein 15 (BMP15) known as FecX on chromosome X. Bone morphogenetic protein (BMP) genes are members of the transforming growth factor-beta (TGF-β) super-family which are multifunctional cytokines with a 2-fold function and are expressed in a variety of cells. BMPs were originally identified based on their ability to produce ectopic bone formation when implanted within the soft tissue in vivo. They also play roles in embryonic development, homeostasis, repairing of various tissue patterning, cell differentiation, and apoptosis. The BMP15 gene regulates granulose cell proliferation and differentiation by promoting granulosa cell mitosis, suppressing follicle-stimulating hormone receptor expression, and stimulating the kit ligand expression; hence, this gene plays a pivotal role in female fertility in mammals. The aim of this study was to investigate tissue-specific mRNA expression profile of BMP15 gene in Raini cashmere goat using Real Time PCR.
 
Materials and Methods
Tissue samples (3 repetitions of each tissue) were taken from ovary, uterus, uterine horn, oviduct, skeletal muscle, subcutaneous fat, heart, liver, lung, kidney, spleen and adrenal   during slaughter at the slaughterhouse from two goats. RNA extraction from tissues and cDNA synthesis were performed. Relative gene expression of BMP15 was done applying Real Time PCR using SYBR Green method. GAPDH gene was used as housekeeping gene. Pfaffl method was used to analyze achieved data.
 
Results  
BMP15 gene expression in ovary, uterus, uterine horn, oviduct, skeletal muscle, subcutaneous fat, heart, liver, lung, kidney, spleen and adrenal tissues using Real Time PCR in studied goats demonstrated that BMP15 gene is expressed only in the ovary tissue and is not expressed in other studied tissues.
 
Conclusions
Based on the results of the present study and the results of other researchers, it can be concluded that BMP15 gene plays an important role in ovary and as a result in fertility, because in the present study it was found that BMP15 is expressed only in ovary tissue. Therefore, BMP15 is likely to be essential for female fertility, and the results of this study provide a basis for future research to describe the role of BMP15 gene as a candidate gene for better fertility and normal physiology in domestic animals, especially goats.

Keywords


توحیدی نژاد فاطمه، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، نجمی نوری عذرا (1393) مقایسه سطوح مختلف بیان ژنRheb  در بافت های مختلف بز کرکی راینی. مجله بیوتکنولوژی کشاورزی 6(4)، 50-35.
جعفری دره در امیر حسین، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، ریاحی مدوار علی (1395) بررسی بیان ژن CIB4  در بافت­های مختلف گوسفند کرمانی با استفاده از Real Time qPCR. مجله پژوهش در نشخوارکنندگان 4(4)، 132-119.
حسنی محمدنبی، اسدی فوزی مسعود، اسمعیلی زاده کشکوئیه علی، محمدآبادی محمدرضا (1389) آنالیز ژنتیکی صفات رشد در بز کرکی راینی با استفاده از مدل حیوانی چند متغیره. مجله علوم دامی ایران 41، 329-323.
خدابخش زاده رسول، محمدآبادی محمدرضا (1396). چندشکلی نیمه دوم اگزون ۲ ژن GDF9گوسفند نژاد کرمانی به روش PCR-SSCP و توالی‌یابی. فصلنامه پژوهش های علوم دامی 27(3)، ۵۱ -۶۰.
خدابخش زاده رسول، محمدآبادی محمدرضا، اسماعیلی زاده علی و همکاران (1393). مطالعه جهش های موجود در نیمه اول اگزون ۲ ژن GDF۹گوسفند آمیخته نژاد رومانوف و نژاد کرمانی. فصلنامه پژوهشهای علوم دامی ایران، 6(4)، ۳۹5 -۴۰۳.
خدابخش زاده رسول، محمدآبادی محمدرضا، مرادی حسین و همکاران (1394). شناسایی جهش های نقطه ای G― Aدر موقعیت های ۴۷۷ و ۷۲۱ اگزون شماره دو ژن GDF9گوسفند نژاد کرمانی. ژنتیک نوین 10(2)، ۲۵۹ -۲۶۶.
خدابخش زاده رسول، محمدآبادی محمدرضا، مرادی حسین و همکاران (1394). شناسایی جهش های موجود در نیمه اول (منتهی به۵') اگزون ۲ ژن GDF۹ گوسفندان آمیخته حاصل از تلاقی نژادهای رومانوف و لری بختیاری. فصلنامه تحقیقات تولیدات دامی 4(4)، ۱۵ -۲۶.
عسکری ناهید، باقی زاده امین، محمدآبادی محمدرضا (1389). مطالعه تنوع ژنتیکی در چهار جمعیت بز کرکی راینی با استفاده از نشانگرهای ISSR. مجله ژنتیک نوین 5، 56-49.
عسکری ناهید، محمدآبادی محمدرضا، بیگی نصیری محمدتقی و همکاران (1378). مطالعه تنوع ژنتیکی بز کرکی راینی با استفاده از نشانگرهای ربزماهواره. مجله دانش کشاورزی 18، 161-155.
علینقی­زاده روح­الله، محمدآبادی محمدرضا، زکی­زاده سونیا (1389) چند شکلی اگزون 2 ژن BMP15 در بز قرمز جبال بارز. مجله بیوتکنولوژی کشاورزی 2(1)،80-69.
محمدآبادی محمدرضا (1390) ارتباط چندشکلی ژن IGFBP-3 با صفات کرک در بز کرکی راینی. مجله ژنتیک نوین 7، 120-115.
محمدآبادی محمدرضا (1398) بیان ژن کالپاستاتین در بز کرکی راینی با استفاده از Real Time PCR. مجله بیوتکنولوژی کشاورزی 11(4)،234-219.
محمدآبادی محمدرضا (1398). بیان ژن Dlk1 در بز کرکی راینی با استفاده از Real Time PCR . مجله بیوتکنولوژی کشاورزی 11(3)، 205-191.
محمدآبادی محمدرضا‎ (1399). بیان ژن ESR1 در بز کرکی راینی با استفاده از Real Time PCR‎. مجله بیوتکنولوژی کشاورزی 12 (1)، 177-192‎.
محمدی نژاد فاطمه، محمدآبادی محمدرضا، برازنده ارسلان (1396). برآورد فراسنجه های ژنتیکی زنده مانی در بزغاله های کرکی راینی با استفاده از دو مدل خطی و ویبال‎. علوم دامی ایران (علوم کشاورزی ایران) 48 (2)، 297-304.
محمودی مریم ، آیت اللهی مهرجردی احمد ، محمدآبادی محمدرضا (1398). تنوع ژنتیکی ژن کاپاکازئین درجمعیت بزهای کرکی راینی، سانن و وحشی با تکنیکPCR-RFLP . ژنتیک نوین 14(1)، ۷۳-۷۷.
هادی زاده مرتضی، محمدآبادی محمدرضا، نیازی علی و همکاران (1392). استفاده از ابزارهای بیوانفورماتیکی در مطالعه اگزون شماره ۲ ژن GDF۹ در بزهای تالی و بیتال. ژنتیک نوین 8(3)، 288-283.
هادی زاده مرتضی، نیازی علی، محمدآبادی محمدرضا و همکاران (1393). بررسی بیوانفورماتیکی اگزون شماره دو ژن BMP15 در بزهای تالی و بیتال. ژنتیک نوین 9(1)، 120-117.
 
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