Identification and isolation of cinnamate 4-hydroxylase and chalcon syntase genes in the roots of scrophularia striata and study of their expression and some physiological traits under the influence of various abiotic elicitors

Document Type : Research Paper

Authors

1 ph-d student of plant breeeding, faculty of agriculture, ilam university

2 Associate Professor Agriculture and Plant Breeding Ilam University

Abstract

Objective
Scrophularia striata is a plant belonging to the Scrophulariaceae family. Due to the importance of phenylpropanoid compounds in this plant, identification and isolation of genes encoding cinnamate enzymes 4-hydroxylase (C4H) and chalcone synthetase (CHS) involved in the biosynthesis of these compounds and also, their expression under the influence of various abiotic elicitors was performed.
Materials and methods
Seeds of wild plants grown around the university were collected and were cultured in greenhouse conditions. Sampling was done before flowering stage. RNA extraction, cDNA synthesis, isolation and sequencing of CHS and C4H genes in the roots of this plant were successfully performed for the first time. The expression of these genes was performed under the influence of three salicylic acid (SA), jasmonic acid (JA) and gibberellic acid (GA) each at concentrations of 100 and 300 ppm using a completely randomized design at the transcript level by Real Time PCR method.
 
 
Results
The results showed that among the elicitors used, JA and SA with the concentration of 100 ppm had the greatest effect on increasing the expression of C4H and CHS genes so that in investigating the effect of the above-mentioned elicitors on the accumulation of phenylpropanoid compounds, this issue was partially confirmed. Also, in the study, increasing the concentration of JA and SA decreased the expression of C4H and CHS genes.
Conclusions
The general results of the use of elicitors on gene expression and accumulation of secondary metabolites showed that the external use of stimulants in appropriate concentrations, possibly through the induction of the plant immune system, can play a role in the biosynthesis and accumulation of valuable secondary metabolites such as phenylpropanoid compounds in the roots of Scrophularia striata and subsequently identifying and analyzing the expression of genes involved in the biosynthesis of such valuable compounds can be effective in complementary basic research for use in the pharmaceutical and food industries.

Keywords


احسنی محمدرضا؛ محمدآبادی محمدرضا؛ اسدی فوزی و همکاران (1398) بیان ژن لپتین در بافت چربی زیرپوستی گاوهای هلشتاین با استفاده از Real Time PCR. مجله بیوتکنولوژی کشاورزی 11، 150-135.
توحیدی نژاد فاطمه؛ محمدآبادی محمدرضا؛ اسمعیلی زاده کشکوئیه علی؛ نجمی نوری عذرا (1393) مقایسه سطوح مختلف بیان ژنRheb  در بافت های مختلف بز کرکی راینی. مجله بیوتکنولوژی کشاورزی 4، 50-35.
جعفری دره در امیر حسین؛ محمدآبادی محمدرضا؛ اسمعیلی زاده کشکوئیه علی؛ ریاحی مدوار علی (1395) بررسی بیان ژن CIB4  در بافت­های مختلف گوسفند کرمانی با استفاده از Real Time qPCR. مجله پژوهش در نشخوارکنندگان 4، 132-119.
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خانپور اردستانی نرگس (1392) بررسی اثر فنیل آلانین و متیل جاسمونات بر میزان اکتیوزید، فعالیت آنزیم و بیان ژن فنیل آلانین آمونیا لیاز (PAL) در کشت سلول .Scrophularia striata رساله دکتری، دانشگاه تربیت مدرس. 130 صفحه.
رهنما حسن؛ حسنلو طاهره؛ شمس محمد رضا (1390) انتقال ژن چالکون سنتاز (CHS)  به ریشه‌های مویین گیاه خار مریم (Silybum marianum) . پژوهشکده بیوتکنولوژی کشاورزی کرج، ایران. 78 ص.
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شرافتی چالشتری رضا؛ شرافتی چالشتری فرهاد؛ شرافتی چالشتری علی؛ اشرفی کورش (1388) بررسی اثر ضد میکروبی و تعیین میزان ترکیبات فنولی، فلاونوئیدی و فلاونولی عصاره اتانولی گل میمونی (Scrophularia striata). مجله دانشگاه علوم پزشکی شهرکرد 11، 32-37.
شوهانی بهناز؛ طاهری مقدم میهن (1388) بررسی اثر التیام بخشی عصاره هیدروالکلی گیاه تشنه داری (Scrophularia striata)  برروی زخم باز پوستی خرگوش. مجله علمی پژوهشی دانشگاه علوم پزشکی ایلام 17، 9-16.
غلامی علی اکبر (1396) تولید متابولیت های ثانویه از طریق مهندسی ژنتیک و کشت بافت. فصلنامه علمی ایمنی زیستی 10، 17-36.
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قبادی سارا؛ معروفی اسعد؛ مجدی محمد (1395) مطالعه بیان ژن‌های کلیدی در بیوسنتز مونوترپن‌ها در بافت‌های مختلف و در پاسخ به الیسیتورهای غیر زیستی در گیاه دارویی مرزه تابستانه (Satureja hortensis). مجله سلول و بافت 7، 292-275.
قهرمان احمد (1365) فلور رنگی ایران. انتشارات موسسه تحقیقات جنگل‌ها و مراتع، 845 ص.
کمالی پورآزاد مریم؛ شریفی محسن؛ زارع مایوان حسن؛ بهمنمنش مهرداد،؛ احمدیان چاشمی نجمه (1396) افزایش بیان ژن آنزیمp -کومارات 3- هیدروکسیلاز و ترکیبات فنیل پروپانوئیدی تحت تاثیر کیتوزان در کشت سلولی گل میمونی سازویی (Scrophularia striata). نشریه فرآیند و کارکرد گیاهی 6، 81-90.
محمدآبادی محمدرضا؛ کرد محبوبه؛ نظری محمود (1397) مطالعه بیان ژن لپتین در بافت‌های مختلف گوسفند کرمانی با استفاده ازReal Time PCR. مجله بیوتکنولوژی کشاورزی 10، 122-111.
نصری سیما؛ چراغی جواد؛ سلطان بیگی سهیلا (1392) بررسی اثر ضد دردی و ضد التهابی عصاره هیدروالکی ریشه و ساقه گیاه گل میمون سازوئی (Scrophularia Striata) در موش کوچک آزمایشگاهی. فصلنامه علمی - پژوهشی تحقیقات گیاهان دارویی و معطر ایران 29، 74-84.
هواسیان محمدرضا؛ پناهی جعفر؛ پاکزاد ایرج؛ داودیان عبداله؛ جلیلیان آناهیتا؛ زمانیان عضدی مونا (1391) بررسی اثر مهاری عصاره هیدروالکلی گیاه تشنه داری (Scrophularia striata) بر روی کاندیدا آلبیکنس در شرایط آزمایشگاهی. فصلنامه علمی و پژوهشی دانشکده پزشکی دانشگاه علوم پزشکی و خدماتی درمانی شهید بهشتی 36، 19-23.
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