The combined effects of salinity and heat on the yield of rosmarinic acid and expression of some genes involved in its biosynthesis in Mentha piperita L. at different time intervals

Document Type : Research Paper

Authors

1 Department of Arid Land and Desert Management. Faculty of Natural Resources and Desert Studies, Yazd University, Yazd

2 Department of environmental science, Faculty of Natural Resources, Yazd University, Yazd

3 3- Department of Arid Land and Desert Management. Faculty of Natural Resources and Desert Studies, Yazd University, Yazd.

4 Yazd university, Yazd, Iran.

5 5- Department of Soil Sciences, Faculty of Natural Resources, Yazd University, Yazd

Abstract

Objective
The growth and yield of plants in the arid and semiarid areas are limited by salinity stress. In these areas, salinity intensifies by high temperatures. Peppermint (Mentha piperita L.) is of great economic importance in the most of world areas due to their valuable essential oils. This study investigated the simultaneous effects of salinity and heat on rosmarinic acid production and expression of three effective genes (C4H, HPPR, and RAS) in rosmarinic biosynthesis in peppermint.
Materials and methods
The effect of two levels of salinity 60 and 120 mM of sodium chloride (irrigation water with salinity of 10 Mm as control) and heat stress at 35°C (25°C as control) at three interval times (24, 48 and 72 hours) after treatments were measured in a completely randomized design with three replications.
Results
The results showed that salinity stress decreased the relative level of rosmarinic acid. The process intensified over time so that it reached its lowest level at salinity of 60 mM at 72 hours. RAS genes expression was significantly reduced under salinity of 120 mM and 60 mM under 25˚C and 35 ˚C. Changes in the expression level of C4H in peppermint seedlings at the salinity levels of 60 mM and 120 mM at 25˚C showed a significant increase (4.1 and 3.6 times respectively) at the first 24 hours compared to control. The expression of HPPR gene in peppermint seedlings at 35 ° C increased 2.68, 1.79 and 2.55 times at 24, 48 and 72 hours, respectively, compared to the control.
Conclusions
Simultaneous salinity and heat stress are a limitation factor for growing Mentha piperita L. These factors had a negative effect on the biosynthesis of rosmarinic acid in this plant. Salinity and heat stress decreased the expression of some genes and increased the expression of others in peppermint.

Keywords


عرب پور رق آبادی زهرا، محمدآبادی محمدرضا، خضری امین (1400) الگوی بیانی ژن p32 در بافت‌های ران، دست، راسته و چربی پشت بره کرمانی. مجله بیوتکنولوژی کشاورزی، 13(4)، 183-200.
محمدآبادی محمدرضا (1399) بیان ژن ESR1 در بز کرکی راینی با استفاده از real time PCR‎. مجله بیوتکنولوژی کشاورزی 12(1)، 192-177.
محمدآبادی محمدرضا (1399) پروفایل بیانیmRNA مختص بافت ژن ESR2 در بز. مجله بیوتکنولوژی کشاورزی 12(4)، 184-169.
محمدآبادی محمدرضا، سفلایی محمد (1399). پروفایل بیانی mRNA مختص بافت ژن BMP15 در بز. مجله بیوتکنولوژی کشاورزی 12(3)، 208-191.
محمدآبادی محمدرضا، کرد محبوبه، نظری محمود (1397) مطالعه بیان ژن لپتین در بافت‌های مختلف گوسفند کرمانی با استفاده از real time PCR. مجله بیوتکنولوژی کشاورزی 10(3)، 122-111.
References
Adham A.N (2015) Comparative extraction methods, phytochemical constituents, fluorescence analysis and HPLC validation of rosmarinic acid content in Mentha piperita, Mentha longifolia and Osimum basilicum. J Pharmacogn Phytochem 3, 130-139.
Afkar S, Zand R (2020) Genetic Relationships of Some Mint Species Using Seed Storage Protein Pattern. J Genet Resour 6(1), 12-19.
Alhaithloul HA, Soliman MH, Ameta KL, et al. (2019) Changes in ecophysiology, osmolytes, and secondary metabolites of the medicinal plants of Mentha piperita and Catharanthus roseus subjected to drought and heat stress. Biomolecules 10, 43-64.
Amini Hajiabadi A, Mosleh Arani A, Ghasemi S, et al. (2021). Mining the rhizosphere of halophytic rangeland plants for halotolerant bacteria to improve growth and yield of salinity-stressed wheat. Plant Physiol and Biochem, 163, 139-153.
André CM, Schafleitner R, Legay S et al. (2009) Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry 70, 1107-1116.
Arabpour Z, Mohammadabadi M, Khezri A (2021) The expression pattern of p32 gene in femur, humeral muscle, back muscle and back fat tissues of Kermani lambs. Agric Biotechnol J 13 (4), 183-200 (In Persian).
Cheng S, Yan J, Meng X et al. (2018) Characterization and expression patterns of a cinnamate-4-hydroxylase gene involved in lignin biosynthesis and in response to various stresses and hormonal treatments in Ginkgo biloba. Acta Physiol Plant. 40, 1-12.
Fletcher RS, Slimmon T, McAuley CY, Kott LS (2005) Heat stress reduces the accumulation of rosmarinic acid and the total antioxidant capacity in spearmint (Mentha spicata L). J of Sci and Food Agr 85, 2429-2436.
Gowton CM, Reut M, Carrillo J (2020) Peppermint essential oil inhibits Drosophila suzukii emergence but reduces Pachycrepoideus vindemmiae parasitism rates. Sci Rep 10, 1-10.
Gull A, Lone AA, Wani NUI (2019) Biotic and Abiotic Stresses in Plants, Abiotic and Biotic Stress in Plants, Alexandre Bosco de Oliveira, IntechOpen London UK, pp 1-174.
Khaleghnezhad V, Yousefi AR, Tavakoli A, Farajmand B (2019) Interactive effects of abscisic acid and temperature on rosmarinic acid, total phenolic compounds, anthocyanin, carotenoid and flavonoid content of dragonhead (Dracocephalum moldavica L.). Sci Hortic 250, 302-309.
Li H, Fu Y, Sun H et al. (2017) Transcriptomic analyses reveal biosynthetic genes related to rosmarinic acid in Dracocephalum tanguticum. Sci Rep 7, 1-10.
Lu X, Hao L, Wang F, Huang C (2014) Molecular cloning, characterization and expression analysis of a gene encoding hydroxyphenylpyruvate reductase involved in rosmarinic acid biosynthesis pathway from Perilla frutescens, Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Springer pp. 1807-1820.
Masoudzadeh SH, Mohammadabadi M, Khezri A, et al. (2020) Effects of diets with different levels of fennel (Foeniculum vulgare) seed powder on DLK1 gene expression in brain, adipose tissue, femur muscle and rumen of Kermani lambs. Small Rumin Res 193, e106276.
Mobasseri M, Ostadrahimi A, Tajaddini A et al. (2020) Effects of saffron supplementation on glycemia and inflammation in patients with type 2 diabetes mellitus: A randomized double-blind, placebo-controlled clinical trial study. Diabetes & Metabolic Syndrome, Clinical Research & Reviews. 14(4), 527-534.
Moghaddam GA, Rezayatmand Z, Esfahani MN, Khozaei M (2019) Genetic defense analysis of tomatoes in response to early blight disease, Alternaria alternata. Plant Physiol Biochem 142, 500-509.
Mohammadabadi M (2021) Tissue-specific mRNA expression profile of ESR2 gene in goat. Agric Biotechnol J 12 (4), 167-181 (In Persian).
Mohammadabadi M, Masoudzadeh SH, Khezri A, et al. (2021) Fennel (Foeniculum vulgare) seed powder increases Delta-Like Non-Canonical Notch Ligand 1 gene expression in testis, liver, and humeral muscle tissues of growing lambs. Heliyon 7 (12), e08542.
Mohammadabadi M, Soflaei M (2020) Tissue-specific mRNA expression profile of BMP15 gene in goat. Agric Biotechnol J 12, 191-208 (In Persian).
Mohammadabadi MR (2020) Expression of ESR1 gene in Raini Cashmere goat using Real Time PCR. Agric Biotechnol J 12 (1), 177-192 (In Persian).
Mohammadabadi MR, Kord M, Nazari M (2018) Studying expression of leptin gene in different tissues of Kermani Sheep using Real Time PCR. Agric Biotechnol J 10, 111-122 (In Persian).
Mohammadabadi MR, Tohidinejad F (2017) Charachteristics determination of Rheb gene and protein in Raini Cashmere goat. Iran J Appl Anim Sci 7, 289-295.
Mosleh Arani A, Bakhshi Khaniki G, Nemati N, et al (2011) Investigation on the effect of salinity stress on seed germination of Salsola abarghuensis, Salsola arbuscula and Salsola yazdiana. Iran J Rangelands Forests Plant Breeding Gen Res. 18: 2.267-279.
Mosleh Arany A., De Jong T. J., Kim H. K., et al (2008). Glucosinolates and other metabolites in the leaves of Arabidopsis thaliana from natural populations and their effects on a generalist and a specialist herbivore. Chemoecology, 18, 65-71.
Mosleh Arani A, Naderi M, Goldansaz SM. (2015). Effect of Harvesting Time on Essential Oil Content and Composition of Thymbra spicata. J medicinal plants by- products. 4(1): 51-55.
Mosleh Arani A, Rafiei A, Tabandeh A, et al. (2018). Morphological and physiological responses of root and leave in Gleditschia caspica to salinity stress. Iran J Plant Biol. 9: 4.1-12.
Petersen M (1997) Cytochrome P450 dependent hydroxylation in the biosynthesis of rosmarinic acid in Coleus sp. Phytochemistry 45:1165–1172.
Rahman LU (2020) Ethnomedicinal, phytochemical and pharmacological updates on Peppermint (Mentha× piperita L.)- A review. Phytotherapy Res 34(9), 2088-2139.
Rastegari AA, Nath Yadav A, Yadav N, Tataei Sarshari N (2019) Bioengineering of secondary metabolites, in New and Future Developments in Microbial Biotechnology and Bioengineering., Elsevier 55-68.
Rivero RM, Mestre T C, Mittler R et al. (2014) The combined effect of salinity and heat reveals a specific physiological, biochemical and molecular response in tomato plants. Plant, Cell and Env 37, 1059–1073.
Sadeghi M, Dehghan S, Fischer R et al. (2013) Isolation and characterization of isochorismate synthase and cinnamate 4-hydroxylase during salinity stress, wounding, and salicylic acid treatment in Carthamus tinctorius. Plant Signal Behav 8, e27335.
Saijo Y, Loo EPi (2020) Plant immunity in signal integration between biotic and abiotic stress responses. New Phytol 225, 87-104.
Shahsavari M, Mohammadabadi M, Khezri A, et al. (2021) Correlation between insulin-like growth factor 1 gene expression and fennel (Foeniculum vulgare) seed powder consumption in muscle of sheep. Anim Biotechnol 33, 1-11.
Tehrani MM, Nasr-Esfahani M, Mousavi A et al. (2020) Regulation of related genes promoting resistant in Iris against root rot disease, Fusarium oxysporum. sp gladioli Genomics 112, 3013-3020.
Todorova D, Katerova Z, Dimitrova R et al. (2020) Exogenous spermine application increases quantity of rosmarinic acid and carnosic acid in salt-treated salvia dfficinalis L. plants in pot experiments, Comptes Rendus de l 'Academie Bulg. Des Sci 73.
Vafadar F, Amooaghaie R, Ehsanzadeh P et al. (2020) Melatonin and calcium modulate the production of rosmarinic acid, luteolin, and apigenin in Dracocephalum kotschyi under salinity stress. Phytochemistry 177, 112422.
Wyse AT, Netto CA (2011) Behavioral and neurochemical effects of proline. Metab. Brain Dis 26, e159.