Unexpected Transcription of Human t-PA cDNA in transgenic tobacco plants

Document Type : Research Paper

Authors

Abstract

Recombinant tissue-type plasminogen activator (rt-PA) is used as a fibrin-specific thrombolytic agent for the treatment of various thromboembolic diseases. In this study, we tried to show the expression of transferred cDNA of human t-PA gene in transgenic tobacco plants produced previously by this group. RT-PCR with specific primers for t-PA, were used to assay the transgenic plants. RT-PCR products were observed on agarose gel electrophoresis after ethidium bromide staining. The results showed that the t-PA gene is stably expressed in second generation transgenic plant at the level of mRNA, while no expression was seen in non transgenic plants. These results also indicate an unfamiliar 650 bp transcript from t-PA transgene in plants. The results of homology search by CDD at NCBI database demonstrate that in addition to complete cDNA, an incomplete transcript so observed. This truncated transcript is a part of cDNA of t-PA that has lost the kringles domain while tyrosine-like serine protease is remained. This may results from alternative RNA splicing. The serine protease domain can carry out the role of t-PA alone. So many scientists are trying to clone this fragment only as a drug. Interestingly the plant produces this fragment naturally. This result may be because of alternative splicing of RNA.

Keywords

Full Text

 Domain, Kringle, recombinant t-PA and transgenic tobacco.

 

References

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Agricultural Biotechnology Journal
Volume 3, Issue 1 - Serial Number 1
vol 3.No1 spring and summer 1390
June 2011
Pages 31-41

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