Evaluation of Genetic Diversity of Suaeda aegyptiaca Genotypes in Khuzestan Province Using SCoT Molecular Markers

Document Type : Research Paper

Authors

1 Graduate student of Plant Breeding and Genetic, University of Yasouj, Yasouj, Iran.

2 Yasouj University

3 Assistance Professor, Faculty of Agriculture, University of Shahid Chamran, Ahvaz, Iran

4 Associate Professor, Faculty of Agriculture, University of Yasouj, Yasouj, Iran

10.22103/jab.2024.22888.1549

Abstract

Objective
Suaeda aegyptiaca (Hasselq.) Zohary is a halophyte plant with many nutritional and medicinal uses. There is no information on study and estimation of the genetic diversity of S. aegyptiaca plants in Khuzestan province. So, this study was designed and implemented with the aim of investigating the genetic diversity and genetic relationships among S. aegyptiaca genotypes in Khuzestan province.
Materials and methods
In order to study of genetic diversity and genetic relationships among S. aegyptiaca genotypes, 26 genotypes from two regions of Khuzestan were selected as group one and two based on their DNA quantity and quality from the 71 collected samples. 12 SCoT primers were used to investigate the diversity and genetic relationships among and within populations. Genomic DNA was extracted from leaf tissue of genotypes and DNA amplification was done using 12 SCoT primers, the obtained bands were scored as zero (band absence) and 1 (band presence). Total number of bands, number of polymorphic bands, and percentage of polymorphic bands, band information index, resolution power, polymorphic information content, marker index and Shannon index were calculated.
Results
A total of 103 fragments were amplified, which showed a high percentage of polymorphism. SCoT14 had the highest polymorphic information (0.43) and marker index (4.36). The highest resolution power (Rp) belongs to SCoT13 (13.38). Cluster analysis using UPGMA method classified the genotypes into five groups. Karoun (KAR2) and Gheyzaniyeh (GH1) genotypes, with the highest genetic distance, were suitable for usage in breeding programs. Analysis of molecular variance (AMOVA) showed that genetic diversity within groups was higher than between groups.
Conclusions
Some of the studied markers showed a high ability to distinguish genotypes, also the results indicated a high genetic diversity among the genotypes in terms of the studied SCoT markers. These results can be used in the breeding programs of S. aegyptiaca and related species.

Keywords

References

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Agricultural Biotechnology Journal
Volume 16, Issue 4
January 2025
Pages 99-120

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