Effect of different hormonal combination on In vitro direct shoot regeneration of basil (Ocimum basilicum)

Document Type : Research Paper

Authors

Abstract

Basil (Ocimum basilicum ) is an annual, herbaceous plants belonging to the family of lamiaceae. Basil is used to as a herb, a spice as well as fresh vegetables. The present study aimed to investigate the effects of different concentrations of growth regulators such as auxin and cytokinin to identify the best hormonal concentration to obtain the highest yield of in vitro produced plantlets using nodal explants. Experiment was performed in factorial completely randomized design, using the basic MS. In this experiment the effects of different concentrations of BAP(0, 2/5, 5 and 7/5 mg/l) and indol acetic acid (0, 0/1 and 0/5 mg/l) was studied alone or together. Regenetaion percent, number of regeneration, vitrification and rooting evaluated. The results showed that the highest percentage and number of regeneration obtained in the medium included 2/5 mg/l BAP concentration and 0 mg/l IAA, respectively. Vitrification was increased with increasing BAP concentration, maximum rooting was observed in the medium containing 0/5 mg/l IAA.
 

Keywords


Basil, Nodal, Regeneration, Vitrification, In vitro.

 

Asghari F, Hossieni B, Hassani A, Shirzad H (2012). Effect of explants source and different hormonal combinations on direct regeneration of basil plants (Ocimum basilicum L.). Australian Journal of Agricultural Engineering 3: 12-17.
Bagheri A, Saffari M (1997) In vitro culture of higher plants. Ferdowsi university of mashhad press. 406 pages (In Farsi).
Banu LA, Bari MA (2007). Protocol establishment for multiplication and regeneration of Ocimum Sanctum Linn. An important medicinal plant with higher religious value in bangladesh. Journal of Plant Science 2: 530-537.
Barcelo CJ, Nicolas RG, Sabater GB and Sanchez TR )1988). Fisiologia Vegetal. Piramide, Madri. Boufleuher LM, Schuelter AR, da Luz CL, Antes VA, et al. (2008). In vitro propagation of Cubiu (Solanum sessiliflorum Dunal) with different auxin and cytokinin types and concentrarions. Asian Journal of Plant Science 12: 639-646.
Begum F, Amin MN, Azad M (2002). In vitro rapid clonal propagation of Ocimum basilicum L. Plant Tissue Culture 12: 27-35.
Bicca Dode L, Bobrowski VL, Braga EJB, Seixas FK, Schuch MW (2003). In vitro propagation of Ocimum basilicum L. (Lamiaceae). Acta Scientiarum 2: 435-437.
Bayliss M (1985). Control of cell division in cultured cells. In: Bryant JA and Francis D (eds) The cell division cycle in plants. Cambridge: Cambridge University Press, pp 157-178.
Del-Poza Jc, Lopez-Matas MA, Ramirez-Parra E, Gutierrez C (2005). Hormonal control of the plant cell cycle. Physiologia Plantarum 123: 173-183.
Genkov T, Ivanova I (1995). Effect of cytokinin-active phenylurea derivatives on shoot multiplication, peroxidase and superoxide dimutase activities of in vitro cultured of carnation. Journal of Plant Physiology 21: 73-83.
Gopi C, Ponmurugan P (2006). Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L. Journal of Biotechnology 126: 260-264.
Grattapaglia D, Machado MA (1998). Micropropagation. In: Cultura de Tecidos e Transformacao Genetica de Plantas(Torres AC, Caldas LS and Buso JA, eds.). Embrapa-cnph, Brasilia 183-247.
Hassani A, Omidbeygi (2003). Effect of drought stress on some morphological and metabolical charectristics of basil. Journal of Agricultural Science 12: 47-59 (In Farsi).
Kadota M, Niimi Y (2003). Effects of cytokinin types and their concentration on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tissue and Organ Culture 72: 261-265.
Keverse C, Prat R, Gaspar Th (1987). Vitrification of carnation in vitro: Changes in cell wall mechanical properties, cellulose and lignin content. Plant Growth Regulation 5: 59-66.
Leshem B (1983). Growth of carnation meristems in vitro: anatomical structure of abnormal plantlets and the effect of agar concentration in the medium on their formation. Annual Botanical 52: 413–415.
Makri O, Kintzios S (1999). Protein changes associated with in vitro plant regeneration of Ocimum basilicum L. Poster P11, ISHS Working Group Quality Management in Micropropagation ‘Methods and Markers for Quality Assurance in Micropropagation.’ University College, Cork, Ireland.
Makri O, Kintzios S (2008). Ocimum sp. (Basil): Botany, Cultivation, Pharmaceutical Properties, and Biotechnology. Journal of Herbs, Spices & Medicinal Plants 13: 123-150.
Meira Z, Meir G, Halevy AH (1983). Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tissue and Organ Culture 2: 55-65.
Mendoza MG, Kaeppler HF (2002). Auxin and suger effects on callus induction and plant regeneration Frequencies from mature embryos of wheat (Triticum aestivum L.). In vitro cell Development and Biology of Plant 38: 39-45.
Pattnaik S, Chand PK (1996). In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil). Plant Cell Report 15: 846-850.
Pattnaik SK, Sahoo Y, Chand PK (1995). Clonal propagation of four medicinal plants, Ocimum americanum L. (hoary basil), O. basilicum L. (sweet basil), O. gratissimum L. (shrubby basil) and O. sanctum L. (sacred basil) through in vitro culture of axillary vegetative buds. In vitro Cellular and Developmental Biology 31: 79-81.
Preece JE (1995) Can nutrient salts partially substitute for plant growth regulators? Plant Tissue Culture Biotechnology 1: 26-37.
Sahoo Y, Pattnaik SK, Chand PK (1997). In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (sweet basil) by axillary shoot proliferation. In vitro Plant Breeding 33: 293-296.
Siddique I, Anis M (2008). An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiologiae Plantarum 30: 493-499.
Singh NK, Sehgal CB (1999). Micropropagation of ‘Holy Basil’ (Ocimum sanctum Linn.) from young inflorescences of mature plants. Plant Growth Regulation 29: 161-166.
Wang TL, Everett NP, Gould AR, Street HP (1981). Studies on the control of the cell cycle in cultured plant cells. The effects of cytokinin. Protoplasma 106: 23-35.